Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Isolation and characterization of a novel antifreeze protein from carrot (Daucus carota)
AU - Smallwood, M
AU - Worrall, D
AU - Byass, L
AU - Elias, L
AU - Ashford, D
AU - Doucet, C J
AU - Holt, C
AU - Telford, J
AU - Lillford, P
AU - Bowles, D J
PY - 1999/6/1
Y1 - 1999/6/1
N2 - A modified assay for inhibition of ice recrystallization which allows unequivocal identification of activity in plant extracts is described. Using this assay a novel, cold-induced, 36 kDa antifreeze protein has been isolated from the tap root of cold-acclimated carrot (Daucus carota) plants, This protein inhibits the recrystallization of ice and exhibits thermal-hysteresis activity. The polypeptide behaves as monomer in solution and is N- glycosylated, The corresponding gene is unique in the carrot genome and induced by cold, The antifreeze protein appears to be localized within the apoplast.
AB - A modified assay for inhibition of ice recrystallization which allows unequivocal identification of activity in plant extracts is described. Using this assay a novel, cold-induced, 36 kDa antifreeze protein has been isolated from the tap root of cold-acclimated carrot (Daucus carota) plants, This protein inhibits the recrystallization of ice and exhibits thermal-hysteresis activity. The polypeptide behaves as monomer in solution and is N- glycosylated, The corresponding gene is unique in the carrot genome and induced by cold, The antifreeze protein appears to be localized within the apoplast.
KW - apoplast
KW - cold-on-regulated gene
KW - ice recrystallization inhibition
KW - polygalacturonase inhibitor protein
KW - thermal hysteresis
KW - THERMAL HYSTERESIS PROTEIN
KW - N-LINKED OLIGOSACCHARIDES
KW - ICE RECRYSTALLIZATION
KW - WINTER RYE
KW - PLANT
KW - SEQUENCE
M3 - Journal article
VL - 340
SP - 385
EP - 391
JO - Biochemical Journal
JF - Biochemical Journal
SN - 0264-6021
IS - 2
ER -