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Isolation of a mRNA encoding a nucleoside diphosphate kinase from tomato that is up-regulated by wounding

Research output: Contribution to journalJournal article


<mark>Journal publication date</mark>07/1994
<mark>Journal</mark>Plant Molecular Biology
Issue number4
Number of pages4
Pages (from-to)739-742
<mark>Original language</mark>English


A cDNA clone (TAB2) encoding a nucleoside diphosphate (NDP) kinase has been isolated from a tomato (Lycopersicon esculentum Mill. cv. Ailsa Craig) cDNA library. The clone is 590 bp long and exhibits a high degree of sequence identity with spinach NDP kinases I and II, Pisum sativum NDP kinase I, Arabidopsis thaliana NDP kinase, Drosophila melanogaster NDP kinase, Dictyostelium discoideum NDP kinase and human Nm 23-H1 and Nm23-H2. Northern analysis has revealed that the mRNA encoded by TAB2 is up-regulated in both leaf and stem tissue in response to wounding. The increase is apparent within 1 h of wounding and is not further elevated by application of ethylene. Southern blot analysis indicates that TAB2 is a member of a small gene family.