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Isoprene synthesis protects transgenic tobacco plants from oxidative stress

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Published

Standard

Isoprene synthesis protects transgenic tobacco plants from oxidative stress. / Vickers, Claudia E.; Possell, Malcolm; Cojocariu, Christian et al.
In: Plant, Cell and Environment, Vol. 32, No. 5, 2009, p. 520-531.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Vickers, CE, Possell, M, Cojocariu, C, Velikova, VB, Laothawornkitkul, J, Ryan, A, Mullineaux, PM & Hewitt, CN 2009, 'Isoprene synthesis protects transgenic tobacco plants from oxidative stress', Plant, Cell and Environment, vol. 32, no. 5, pp. 520-531. https://doi.org/10.1111/j.1365-3040.2009.01946.x

APA

Vickers, C. E., Possell, M., Cojocariu, C., Velikova, V. B., Laothawornkitkul, J., Ryan, A., Mullineaux, P. M., & Hewitt, C. N. (2009). Isoprene synthesis protects transgenic tobacco plants from oxidative stress. Plant, Cell and Environment, 32(5), 520-531. https://doi.org/10.1111/j.1365-3040.2009.01946.x

Vancouver

Vickers CE, Possell M, Cojocariu C, Velikova VB, Laothawornkitkul J, Ryan A et al. Isoprene synthesis protects transgenic tobacco plants from oxidative stress. Plant, Cell and Environment. 2009;32(5):520-531. doi: 10.1111/j.1365-3040.2009.01946.x

Author

Vickers, Claudia E. ; Possell, Malcolm ; Cojocariu, Christian et al. / Isoprene synthesis protects transgenic tobacco plants from oxidative stress. In: Plant, Cell and Environment. 2009 ; Vol. 32, No. 5. pp. 520-531.

Bibtex

@article{7ab54218b6bd4b1cb47ea6cbef788716,
title = "Isoprene synthesis protects transgenic tobacco plants from oxidative stress",
abstract = "Isoprene emission represents a significant loss of carbon to those plant species that synthesize this highly volatile and reactive compound. As a tool for studying the role of isoprene in plant physiology and biochemistry, we developed transgenic tobacco plants capable of emitting isoprene in a similar manner to and at rates comparable to a naturally emitting species. Thermotolerance of photosynthesis against transient high-temperature episodes could only be observed in lines emitting high levels of isoprene; the effect was very mild and could only be identified over repetitive stress events. However, isoprene-emitting plants were highly resistant to ozone-induced oxidative damage compared with their non-emitting azygous controls. In ozone-treated plants, accumulation of toxic reactive oxygen species (ROS) was inhibited, and antioxidant levels were higher. Isoprene-emitting plants showed remarkably decreased foliar damage and higher rates of photosynthesis compared to non-emitting plants immediately following oxidative stress events. An inhibition of hydrogen peroxide accumulation in isoprene-emitting plants may stall the programmed cell death response which would otherwise lead to foliar necrosis. These results demonstrate that endogenously produced isoprene provides protection from oxidative damage.",
keywords = "isoprene synthase, oxidative damage, ozone, reactive oxygen species, thermotolerance, REACTION-MASS-SPECTROMETRY, VOLATILE ORGANIC-COMPOUNDS, PTR-MS, INCREASES THERMOTOLERANCE, NEGATIVE CONSEQUENCES, LIPID-PEROXIDATION, COMPOUND EMISSIONS, HYDROGEN-PEROXIDE, LEAVES, OZONE",
author = "Vickers, {Claudia E.} and Malcolm Possell and Christian Cojocariu and Velikova, {Violeta B.} and Jullada Laothawornkitkul and Annette Ryan and Mullineaux, {Philip M.} and Hewitt, {C. N.}",
year = "2009",
doi = "10.1111/j.1365-3040.2009.01946.x",
language = "English",
volume = "32",
pages = "520--531",
journal = "Plant, Cell and Environment",
issn = "0140-7791",
publisher = "Wiley",
number = "5",

}

RIS

TY - JOUR

T1 - Isoprene synthesis protects transgenic tobacco plants from oxidative stress

AU - Vickers, Claudia E.

AU - Possell, Malcolm

AU - Cojocariu, Christian

AU - Velikova, Violeta B.

AU - Laothawornkitkul, Jullada

AU - Ryan, Annette

AU - Mullineaux, Philip M.

AU - Hewitt, C. N.

PY - 2009

Y1 - 2009

N2 - Isoprene emission represents a significant loss of carbon to those plant species that synthesize this highly volatile and reactive compound. As a tool for studying the role of isoprene in plant physiology and biochemistry, we developed transgenic tobacco plants capable of emitting isoprene in a similar manner to and at rates comparable to a naturally emitting species. Thermotolerance of photosynthesis against transient high-temperature episodes could only be observed in lines emitting high levels of isoprene; the effect was very mild and could only be identified over repetitive stress events. However, isoprene-emitting plants were highly resistant to ozone-induced oxidative damage compared with their non-emitting azygous controls. In ozone-treated plants, accumulation of toxic reactive oxygen species (ROS) was inhibited, and antioxidant levels were higher. Isoprene-emitting plants showed remarkably decreased foliar damage and higher rates of photosynthesis compared to non-emitting plants immediately following oxidative stress events. An inhibition of hydrogen peroxide accumulation in isoprene-emitting plants may stall the programmed cell death response which would otherwise lead to foliar necrosis. These results demonstrate that endogenously produced isoprene provides protection from oxidative damage.

AB - Isoprene emission represents a significant loss of carbon to those plant species that synthesize this highly volatile and reactive compound. As a tool for studying the role of isoprene in plant physiology and biochemistry, we developed transgenic tobacco plants capable of emitting isoprene in a similar manner to and at rates comparable to a naturally emitting species. Thermotolerance of photosynthesis against transient high-temperature episodes could only be observed in lines emitting high levels of isoprene; the effect was very mild and could only be identified over repetitive stress events. However, isoprene-emitting plants were highly resistant to ozone-induced oxidative damage compared with their non-emitting azygous controls. In ozone-treated plants, accumulation of toxic reactive oxygen species (ROS) was inhibited, and antioxidant levels were higher. Isoprene-emitting plants showed remarkably decreased foliar damage and higher rates of photosynthesis compared to non-emitting plants immediately following oxidative stress events. An inhibition of hydrogen peroxide accumulation in isoprene-emitting plants may stall the programmed cell death response which would otherwise lead to foliar necrosis. These results demonstrate that endogenously produced isoprene provides protection from oxidative damage.

KW - isoprene synthase

KW - oxidative damage

KW - ozone

KW - reactive oxygen species

KW - thermotolerance

KW - REACTION-MASS-SPECTROMETRY

KW - VOLATILE ORGANIC-COMPOUNDS

KW - PTR-MS

KW - INCREASES THERMOTOLERANCE

KW - NEGATIVE CONSEQUENCES

KW - LIPID-PEROXIDATION

KW - COMPOUND EMISSIONS

KW - HYDROGEN-PEROXIDE

KW - LEAVES

KW - OZONE

U2 - 10.1111/j.1365-3040.2009.01946.x

DO - 10.1111/j.1365-3040.2009.01946.x

M3 - Journal article

VL - 32

SP - 520

EP - 531

JO - Plant, Cell and Environment

JF - Plant, Cell and Environment

SN - 0140-7791

IS - 5

ER -