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Leishmania mexicana: induction of metacyclogenesis by cultivation of promastigotes at acidic pH

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Leishmania mexicana: induction of metacyclogenesis by cultivation of promastigotes at acidic pH. / Bates, P A; Tetley, L.
In: Experimental Parasitology, Vol. 76, No. 4, 06.1993, p. 412-423.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Bates, PA & Tetley, L 1993, 'Leishmania mexicana: induction of metacyclogenesis by cultivation of promastigotes at acidic pH', Experimental Parasitology, vol. 76, no. 4, pp. 412-423. https://doi.org/10.1006/expr.1993.1050

APA

Bates, P. A., & Tetley, L. (1993). Leishmania mexicana: induction of metacyclogenesis by cultivation of promastigotes at acidic pH. Experimental Parasitology, 76(4), 412-423. https://doi.org/10.1006/expr.1993.1050

Vancouver

Bates PA, Tetley L. Leishmania mexicana: induction of metacyclogenesis by cultivation of promastigotes at acidic pH. Experimental Parasitology. 1993 Jun;76(4):412-423. doi: 10.1006/expr.1993.1050

Author

Bates, P A ; Tetley, L. / Leishmania mexicana : induction of metacyclogenesis by cultivation of promastigotes at acidic pH. In: Experimental Parasitology. 1993 ; Vol. 76, No. 4. pp. 412-423.

Bibtex

@article{fcec98cdd50f4f41ad3de5800fdfc048,
title = "Leishmania mexicana: induction of metacyclogenesis by cultivation of promastigotes at acidic pH",
abstract = "Cultivation of recently transformed Leishmania mexicana promastigotes at pH 5.5 in Schneider's Drosophila medium supplemented with 20% fetal calf serum produced a homogeneous stationary phase population morphologically similar to metacyclic forms. The cultured forms developed functional characteristics consistent with being metacyclic: they were resistant to complement-mediated lysis, possessed a discernable surface membrane coat in transmission electron micrographs, and were highly infective to peritoneal macrophages in vitro. In contrast, growth of promastigotes at pH 7.0 produced morphologically mixed populations of stationary phase promastigotes, but including a subpopulation with metacyclic-like morphology. These results provide a method for culturing pure populations of L. mexicana metacyclics and provide evidence that metacyclics are biochemically preadapted for survival at acidic pH as amastigotes in macrophage phagolysosomes.",
author = "Bates, {P A} and L Tetley",
year = "1993",
month = jun,
doi = "10.1006/expr.1993.1050",
language = "English",
volume = "76",
pages = "412--423",
journal = "Experimental Parasitology",
issn = "0014-4894",
publisher = "Academic Press Inc.",
number = "4",

}

RIS

TY - JOUR

T1 - Leishmania mexicana

T2 - induction of metacyclogenesis by cultivation of promastigotes at acidic pH

AU - Bates, P A

AU - Tetley, L

PY - 1993/6

Y1 - 1993/6

N2 - Cultivation of recently transformed Leishmania mexicana promastigotes at pH 5.5 in Schneider's Drosophila medium supplemented with 20% fetal calf serum produced a homogeneous stationary phase population morphologically similar to metacyclic forms. The cultured forms developed functional characteristics consistent with being metacyclic: they were resistant to complement-mediated lysis, possessed a discernable surface membrane coat in transmission electron micrographs, and were highly infective to peritoneal macrophages in vitro. In contrast, growth of promastigotes at pH 7.0 produced morphologically mixed populations of stationary phase promastigotes, but including a subpopulation with metacyclic-like morphology. These results provide a method for culturing pure populations of L. mexicana metacyclics and provide evidence that metacyclics are biochemically preadapted for survival at acidic pH as amastigotes in macrophage phagolysosomes.

AB - Cultivation of recently transformed Leishmania mexicana promastigotes at pH 5.5 in Schneider's Drosophila medium supplemented with 20% fetal calf serum produced a homogeneous stationary phase population morphologically similar to metacyclic forms. The cultured forms developed functional characteristics consistent with being metacyclic: they were resistant to complement-mediated lysis, possessed a discernable surface membrane coat in transmission electron micrographs, and were highly infective to peritoneal macrophages in vitro. In contrast, growth of promastigotes at pH 7.0 produced morphologically mixed populations of stationary phase promastigotes, but including a subpopulation with metacyclic-like morphology. These results provide a method for culturing pure populations of L. mexicana metacyclics and provide evidence that metacyclics are biochemically preadapted for survival at acidic pH as amastigotes in macrophage phagolysosomes.

U2 - 10.1006/expr.1993.1050

DO - 10.1006/expr.1993.1050

M3 - Journal article

C2 - 8513879

VL - 76

SP - 412

EP - 423

JO - Experimental Parasitology

JF - Experimental Parasitology

SN - 0014-4894

IS - 4

ER -