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LFASS: Label-Free Automated Survival Scoring for High-Throughput Nematode Assays

Research output: Contribution to journalJournal article

Published
  • Alex Benedetto
  • Timothee Bambade
  • Catherine Au
  • Jennifer Tullet
  • Hairuo Dang
  • Kalina Cetnar
  • Filipe Cabreiro
  • David Gems
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<mark>Journal publication date</mark>28/09/2017
<mark>Journal</mark>Biorxiv
Number of pages23
Publication StatusPublished
<mark>Original language</mark>English

Abstract

Caenorhabditis elegans is an excellent model for high-throughput experimental approaches, but lacks a robust, versatile and automated means to pinpoint time of death. Here we describe an automated, label-free, high-throughput method using death-associated fluorescence to monitor nematode survival, which we apply to stress and infection resistance assays. We demonstrate its use to define correlations between age, longevity and stress-resistance, and reveal an autophagy-dependent increase in acute stress resistance in early adulthood.