Final published version
Licence: CC BY-NC-ND: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
Research output: Contribution to Journal/Magazine › Journal article
Research output: Contribution to Journal/Magazine › Journal article
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TY - JOUR
T1 - LFASS: Label-Free Automated Survival Scoring for High-Throughput Nematode Assays
AU - Benedetto, Alex
AU - Bambade, Timothee
AU - Au, Catherine
AU - Tullet, Jennifer
AU - Dang, Hairuo
AU - Cetnar, Kalina
AU - Cabreiro, Filipe
AU - Gems, David
PY - 2017/9/28
Y1 - 2017/9/28
N2 - Caenorhabditis elegans is an excellent model for high-throughput experimental approaches, but lacks a robust, versatile and automated means to pinpoint time of death. Here we describe an automated, label-free, high-throughput method using death-associated fluorescence to monitor nematode survival, which we apply to stress and infection resistance assays. We demonstrate its use to define correlations between age, longevity and stress-resistance, and reveal an autophagy-dependent increase in acute stress resistance in early adulthood.
AB - Caenorhabditis elegans is an excellent model for high-throughput experimental approaches, but lacks a robust, versatile and automated means to pinpoint time of death. Here we describe an automated, label-free, high-throughput method using death-associated fluorescence to monitor nematode survival, which we apply to stress and infection resistance assays. We demonstrate its use to define correlations between age, longevity and stress-resistance, and reveal an autophagy-dependent increase in acute stress resistance in early adulthood.
KW - Nematodes
KW - high-throughput
KW - autophagy
KW - oxidative stress
KW - elegans
KW - automated analysis
KW - survival
KW - heat-shock
KW - infection
KW - ageing
M3 - Journal article
JO - Biorxiv
JF - Biorxiv
ER -