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    Rights statement: © 2015 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

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Life history correlates of faecal bacterial species richness in a wild population of the blue tit Cyanistes caeruleus

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Life history correlates of faecal bacterial species richness in a wild population of the blue tit Cyanistes caeruleus. / Benskin, Clare; Rhodes, Glenn; Pickup, Roger; Mainwaring, Mark; Wilson, Ken; Hartley, Ian.

In: Ecology and Evolution, Vol. 5, No. 4, 02.2015, p. 821-835.

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@article{137a3cf895224eda9b1bef70415743fe,
title = "Life history correlates of faecal bacterial species richness in a wild population of the blue tit Cyanistes caeruleus",
abstract = "Very little is known about the normal gastrointestinal flora of wild birds, or how it might affect or reflect the host's life-history traits. The aim of this study was to survey the species richness of bacteria in the feces of a wild population of blue tits Cyanistes caeruleus and to explore the relationships between bacterial species richness and various life-history traits, such as age, sex, and reproductive success. Using PCR-TGGE, 55 operational taxonomic units (OTUs) were identified in blue tit feces. DNA sequencing revealed that the 16S rRNA gene was amplified from a diverse range of bacteria, including those that shared closest homology with Bacillus licheniformis, Campylobacter lari, Pseudomonas spp., and Salmonella spp. For adults, there was a significant negative relationship between bacterial species richness and the likelihood of being detected alive the following breeding season; bacterial richness was consistent across years but declined through the breeding season; and breeding pairs had significantly more similar bacterial richness than expected by chance alone. Reduced adult survival was correlated with the presence of an OTU most closely resembling C. lari; enhanced adult survival was associated with an OTU most similar to Arthrobacter spp. For nestlings, there was no significant change in bacterial species richness between the first and second week after hatching, and nestlings sharing the same nest had significantly more similar bacterial richness. Collectively, these results provide compelling evidence that bacterial species richness was associated with several aspects of the life history of their hosts.",
keywords = "16S rRNA, bacterial species richness, fecal microbial community analysis, life-history traits, operational taxonomic unit, PCR-TGGE, RED-WINGED BLACKBIRDS, 16S RIBOSOMAL-RNA, BROILER-CHICKENS, PASSERINE BIRD, GASTROINTESTINAL MICROFLORA, CAMPYLOBACTER-JEJUNI, MICROBIAL DIVERSITY, BODY CONDITION, COMMUNITIES, SOIL",
author = "Clare Benskin and Glenn Rhodes and Roger Pickup and Mark Mainwaring and Ken Wilson and Ian Hartley",
note = "{\textcopyright} 2015 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited. ",
year = "2015",
month = feb
doi = "10.1002/ece3.1384",
language = "English",
volume = "5",
pages = "821--835",
journal = "Ecology and Evolution",
issn = "2045-7758",
publisher = "John Wiley and Sons Ltd",
number = "4",

}

RIS

TY - JOUR

T1 - Life history correlates of faecal bacterial species richness in a wild population of the blue tit Cyanistes caeruleus

AU - Benskin, Clare

AU - Rhodes, Glenn

AU - Pickup, Roger

AU - Mainwaring, Mark

AU - Wilson, Ken

AU - Hartley, Ian

N1 - © 2015 The Authors. Ecology and Evolution published by John Wiley & Sons Ltd. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.

PY - 2015/2

Y1 - 2015/2

N2 - Very little is known about the normal gastrointestinal flora of wild birds, or how it might affect or reflect the host's life-history traits. The aim of this study was to survey the species richness of bacteria in the feces of a wild population of blue tits Cyanistes caeruleus and to explore the relationships between bacterial species richness and various life-history traits, such as age, sex, and reproductive success. Using PCR-TGGE, 55 operational taxonomic units (OTUs) were identified in blue tit feces. DNA sequencing revealed that the 16S rRNA gene was amplified from a diverse range of bacteria, including those that shared closest homology with Bacillus licheniformis, Campylobacter lari, Pseudomonas spp., and Salmonella spp. For adults, there was a significant negative relationship between bacterial species richness and the likelihood of being detected alive the following breeding season; bacterial richness was consistent across years but declined through the breeding season; and breeding pairs had significantly more similar bacterial richness than expected by chance alone. Reduced adult survival was correlated with the presence of an OTU most closely resembling C. lari; enhanced adult survival was associated with an OTU most similar to Arthrobacter spp. For nestlings, there was no significant change in bacterial species richness between the first and second week after hatching, and nestlings sharing the same nest had significantly more similar bacterial richness. Collectively, these results provide compelling evidence that bacterial species richness was associated with several aspects of the life history of their hosts.

AB - Very little is known about the normal gastrointestinal flora of wild birds, or how it might affect or reflect the host's life-history traits. The aim of this study was to survey the species richness of bacteria in the feces of a wild population of blue tits Cyanistes caeruleus and to explore the relationships between bacterial species richness and various life-history traits, such as age, sex, and reproductive success. Using PCR-TGGE, 55 operational taxonomic units (OTUs) were identified in blue tit feces. DNA sequencing revealed that the 16S rRNA gene was amplified from a diverse range of bacteria, including those that shared closest homology with Bacillus licheniformis, Campylobacter lari, Pseudomonas spp., and Salmonella spp. For adults, there was a significant negative relationship between bacterial species richness and the likelihood of being detected alive the following breeding season; bacterial richness was consistent across years but declined through the breeding season; and breeding pairs had significantly more similar bacterial richness than expected by chance alone. Reduced adult survival was correlated with the presence of an OTU most closely resembling C. lari; enhanced adult survival was associated with an OTU most similar to Arthrobacter spp. For nestlings, there was no significant change in bacterial species richness between the first and second week after hatching, and nestlings sharing the same nest had significantly more similar bacterial richness. Collectively, these results provide compelling evidence that bacterial species richness was associated with several aspects of the life history of their hosts.

KW - 16S rRNA

KW - bacterial species richness

KW - fecal microbial community analysis

KW - life-history traits

KW - operational taxonomic unit

KW - PCR-TGGE

KW - RED-WINGED BLACKBIRDS

KW - 16S RIBOSOMAL-RNA

KW - BROILER-CHICKENS

KW - PASSERINE BIRD

KW - GASTROINTESTINAL MICROFLORA

KW - CAMPYLOBACTER-JEJUNI

KW - MICROBIAL DIVERSITY

KW - BODY CONDITION

KW - COMMUNITIES

KW - SOIL

U2 - 10.1002/ece3.1384

DO - 10.1002/ece3.1384

M3 - Journal article

VL - 5

SP - 821

EP - 835

JO - Ecology and Evolution

JF - Ecology and Evolution

SN - 2045-7758

IS - 4

ER -