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    Rights statement: This is the author’s version of a work that was accepted for publication in Rhizosphere. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Rhizosphere, 3, 1, 2017 DOI: 10.1016/j.rhisph.2016.11.004

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Linking the depletion of rhizosphere phosphorus to the heterologous expression of a fungal phytase in Nicotiana tabacum as revealed by enzyme-labile P and solution 31P NMR spectroscopy

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Linking the depletion of rhizosphere phosphorus to the heterologous expression of a fungal phytase in Nicotiana tabacum as revealed by enzyme-labile P and solution 31P NMR spectroscopy. / Giles, Courtney D.; George, Timothy S.; Brown, Lawrie K. et al.
In: Rhizosphere, Vol. 3, No. 1, 06.2017, p. 82-91.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Giles, CD, George, TS, Brown, LK, Mezeli, M, Shand, CA, Richardson, AE, Mackay, R, Wendler, R, Darch, T, Menezes-Blackburn, D, Cooper, P, Stutter, MI, Lumsdon, DG, Blackwell, MSA, Wearing, C, Zhang, H & Haygarth, PM 2017, 'Linking the depletion of rhizosphere phosphorus to the heterologous expression of a fungal phytase in Nicotiana tabacum as revealed by enzyme-labile P and solution 31P NMR spectroscopy', Rhizosphere, vol. 3, no. 1, pp. 82-91. https://doi.org/10.1016/j.rhisph.2016.11.004

APA

Giles, C. D., George, T. S., Brown, L. K., Mezeli, M., Shand, C. A., Richardson, A. E., Mackay, R., Wendler, R., Darch, T., Menezes-Blackburn, D., Cooper, P., Stutter, M. I., Lumsdon, D. G., Blackwell, M. S. A., Wearing, C., Zhang, H., & Haygarth, P. M. (2017). Linking the depletion of rhizosphere phosphorus to the heterologous expression of a fungal phytase in Nicotiana tabacum as revealed by enzyme-labile P and solution 31P NMR spectroscopy. Rhizosphere, 3(1), 82-91. https://doi.org/10.1016/j.rhisph.2016.11.004

Vancouver

Giles CD, George TS, Brown LK, Mezeli M, Shand CA, Richardson AE et al. Linking the depletion of rhizosphere phosphorus to the heterologous expression of a fungal phytase in Nicotiana tabacum as revealed by enzyme-labile P and solution 31P NMR spectroscopy. Rhizosphere. 2017 Jun;3(1):82-91. Epub 2016 Dec 8. doi: 10.1016/j.rhisph.2016.11.004

Author

Giles, Courtney D. ; George, Timothy S. ; Brown, Lawrie K. et al. / Linking the depletion of rhizosphere phosphorus to the heterologous expression of a fungal phytase in Nicotiana tabacum as revealed by enzyme-labile P and solution 31P NMR spectroscopy. In: Rhizosphere. 2017 ; Vol. 3, No. 1. pp. 82-91.

Bibtex

@article{14a250540f3e454da1b0b02d7ea208a2,
title = "Linking the depletion of rhizosphere phosphorus to the heterologous expression of a fungal phytase in Nicotiana tabacum as revealed by enzyme-labile P and solution 31P NMR spectroscopy",
abstract = "Root exudation of phytase could improve the ability of plants to access organic forms of soil phosphorus (P), thereby minimizing fertilizer requirements and improving P use efficiency in agroecosystems. After 75 days growth in a high available P soil, shoot biomass and P accumulation, soil pH, and rhizosphere P depletion were investigated in Nicotiana tabacum wild-type and transgenic plant-lines expressing and exuding Aspergillus niger phytase (ex::phyA), or a null-vector control. Solution 31P NMR analysis revealed a 7% to 11% increase in orthophosphate and a comparable depletion of undefined monoester P compounds (-13 to -18%) in the rhizosphere of tobacco plants relative to the unplanted soil control. Wild-type plants had the greatest impact on the composition of rhizosphere P based on the depletion of other monoester P, polyphosphate, and phosphonate species. The depletion of phytase-labile P by ex::phyA plants was associated with decreased proportions of other monoester P, rather than myo-InsP6 as expected. Rhizosphere pH increased from 6.0 to 6.5–6.7 in transgenic plant soils, beyond the pH optimum for A. niger phyA activity (pH=5), and may explain the limited specificity of ex::phyA plants for phytate in this soil. The efficacy of single exudation traits (e.g., phytase) therefore appear to be limited in P-replete soil conditions and may be improved where soil pH matches the functional requirements of the enzyme or trait of interest.",
keywords = "Phosphorus, Phytate, Phytase, Tobacco, 31P NMR spectroscopy, pH",
author = "Giles, {Courtney D.} and George, {Timothy S.} and Brown, {Lawrie K.} and Malika Mezeli and Shand, {Charles A.} and Richardson, {Alan E.} and Regina Mackay and Renate Wendler and Tegan Darch and Daniel Menezes-Blackburn and Pat Cooper and Stutter, {Marc I.} and Lumsdon, {David G.} and Blackwell, {Martin S.A.} and Catherine Wearing and Hao Zhang and Haygarth, {Philip M.}",
note = "This is the author{\textquoteright}s version of a work that was accepted for publication in Rhizosphere. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Rhizosphere, 3, 1, 2017 DOI: 10.1016/j.rhisph.2016.11.004",
year = "2017",
month = jun,
doi = "10.1016/j.rhisph.2016.11.004",
language = "English",
volume = "3",
pages = "82--91",
journal = "Rhizosphere",
issn = "2452-2198",
publisher = "Elsevier BV",
number = "1",

}

RIS

TY - JOUR

T1 - Linking the depletion of rhizosphere phosphorus to the heterologous expression of a fungal phytase in Nicotiana tabacum as revealed by enzyme-labile P and solution 31P NMR spectroscopy

AU - Giles, Courtney D.

AU - George, Timothy S.

AU - Brown, Lawrie K.

AU - Mezeli, Malika

AU - Shand, Charles A.

AU - Richardson, Alan E.

AU - Mackay, Regina

AU - Wendler, Renate

AU - Darch, Tegan

AU - Menezes-Blackburn, Daniel

AU - Cooper, Pat

AU - Stutter, Marc I.

AU - Lumsdon, David G.

AU - Blackwell, Martin S.A.

AU - Wearing, Catherine

AU - Zhang, Hao

AU - Haygarth, Philip M.

N1 - This is the author’s version of a work that was accepted for publication in Rhizosphere. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Rhizosphere, 3, 1, 2017 DOI: 10.1016/j.rhisph.2016.11.004

PY - 2017/6

Y1 - 2017/6

N2 - Root exudation of phytase could improve the ability of plants to access organic forms of soil phosphorus (P), thereby minimizing fertilizer requirements and improving P use efficiency in agroecosystems. After 75 days growth in a high available P soil, shoot biomass and P accumulation, soil pH, and rhizosphere P depletion were investigated in Nicotiana tabacum wild-type and transgenic plant-lines expressing and exuding Aspergillus niger phytase (ex::phyA), or a null-vector control. Solution 31P NMR analysis revealed a 7% to 11% increase in orthophosphate and a comparable depletion of undefined monoester P compounds (-13 to -18%) in the rhizosphere of tobacco plants relative to the unplanted soil control. Wild-type plants had the greatest impact on the composition of rhizosphere P based on the depletion of other monoester P, polyphosphate, and phosphonate species. The depletion of phytase-labile P by ex::phyA plants was associated with decreased proportions of other monoester P, rather than myo-InsP6 as expected. Rhizosphere pH increased from 6.0 to 6.5–6.7 in transgenic plant soils, beyond the pH optimum for A. niger phyA activity (pH=5), and may explain the limited specificity of ex::phyA plants for phytate in this soil. The efficacy of single exudation traits (e.g., phytase) therefore appear to be limited in P-replete soil conditions and may be improved where soil pH matches the functional requirements of the enzyme or trait of interest.

AB - Root exudation of phytase could improve the ability of plants to access organic forms of soil phosphorus (P), thereby minimizing fertilizer requirements and improving P use efficiency in agroecosystems. After 75 days growth in a high available P soil, shoot biomass and P accumulation, soil pH, and rhizosphere P depletion were investigated in Nicotiana tabacum wild-type and transgenic plant-lines expressing and exuding Aspergillus niger phytase (ex::phyA), or a null-vector control. Solution 31P NMR analysis revealed a 7% to 11% increase in orthophosphate and a comparable depletion of undefined monoester P compounds (-13 to -18%) in the rhizosphere of tobacco plants relative to the unplanted soil control. Wild-type plants had the greatest impact on the composition of rhizosphere P based on the depletion of other monoester P, polyphosphate, and phosphonate species. The depletion of phytase-labile P by ex::phyA plants was associated with decreased proportions of other monoester P, rather than myo-InsP6 as expected. Rhizosphere pH increased from 6.0 to 6.5–6.7 in transgenic plant soils, beyond the pH optimum for A. niger phyA activity (pH=5), and may explain the limited specificity of ex::phyA plants for phytate in this soil. The efficacy of single exudation traits (e.g., phytase) therefore appear to be limited in P-replete soil conditions and may be improved where soil pH matches the functional requirements of the enzyme or trait of interest.

KW - Phosphorus

KW - Phytate

KW - Phytase

KW - Tobacco

KW - 31P NMR spectroscopy

KW - pH

U2 - 10.1016/j.rhisph.2016.11.004

DO - 10.1016/j.rhisph.2016.11.004

M3 - Journal article

VL - 3

SP - 82

EP - 91

JO - Rhizosphere

JF - Rhizosphere

SN - 2452-2198

IS - 1

ER -