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Locomotor and geotactic behavior of Drosophila melanogaster over-expressing neprilysin 2

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Locomotor and geotactic behavior of Drosophila melanogaster over-expressing neprilysin 2. / Bland, Nicholas D.; Robinson, Philip; Thomas, Josie E.; Shirras, Alan D.; Turner, Anthony J.; Isaac, R. Elwyn.

In: Peptides, Vol. 30, No. 3, 03.2009, p. 571-574.

Research output: Contribution to journalJournal article

Harvard

Bland, ND, Robinson, P, Thomas, JE, Shirras, AD, Turner, AJ & Isaac, RE 2009, 'Locomotor and geotactic behavior of Drosophila melanogaster over-expressing neprilysin 2', Peptides, vol. 30, no. 3, pp. 571-574. https://doi.org/10.1016/j.peptides.2008.10.020

APA

Bland, N. D., Robinson, P., Thomas, J. E., Shirras, A. D., Turner, A. J., & Isaac, R. E. (2009). Locomotor and geotactic behavior of Drosophila melanogaster over-expressing neprilysin 2. Peptides, 30(3), 571-574. https://doi.org/10.1016/j.peptides.2008.10.020

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Author

Bland, Nicholas D. ; Robinson, Philip ; Thomas, Josie E. ; Shirras, Alan D. ; Turner, Anthony J. ; Isaac, R. Elwyn. / Locomotor and geotactic behavior of Drosophila melanogaster over-expressing neprilysin 2. In: Peptides. 2009 ; Vol. 30, No. 3. pp. 571-574.

Bibtex

@article{b7a2849f2da649e8b853482a95b74976,
title = "Locomotor and geotactic behavior of Drosophila melanogaster over-expressing neprilysin 2",
abstract = "The neprilysin (M13) family of zinc-metallopeptidases has been implicated in a variety of physiological processes, but principally the control of neuropeptide levels in a range of animal species. The overexpression of the amyloid-degrading enzyme, neprilysin, as a therapeutic strategy for Alzheimer's disease is a concept that is gaining in popularity. Here we utilize the GAL4/UAS system to over-express the Drosophila melanogaster Nep2 gene, a close homologue of neprilysin, in flies yielding an increase in NEP2 protein that is detectable by both immunoblotting and enzyme activity. This increase in NEP2 caused a behavioral phenotype manifested in abnormal climbing behavior. Wild type flies climb in a linear, vertical path, but NEP2 over-expressing (Nep2(OEX)) flies tend to climb in a spiral pattern and display an increase in grooming behavior during frequent stationary periods. Nep2(OEX) flies also perform poorly in a geotaxis maze, taking ten times as long to complete the course compared to wild type Drosophila. We hypothesize that the poor performance of the Nep2(OEX) flies in locomotor assays is due to perturbation of neuropeptide signaling and provides evidence of detrimental effects of neprilysin over-expression. (c) 2008 Elsevier Inc. All rights reserved.",
keywords = "Drosophila melanogaster, Neprilysin, Alzheimer's disease, Insect behavior, Neuropeptide metabolism, ENDOTHELIN-CONVERTING ENZYME, AMYLOID-BETA PEPTIDE, ALZHEIMERS-DISEASE, THERAPEUTIC TARGETS, A-BETA, MODEL, ENDOPEPTIDASE, DEGRADATION, PATHOLOGY, TOXICITY",
author = "Bland, {Nicholas D.} and Philip Robinson and Thomas, {Josie E.} and Shirras, {Alan D.} and Turner, {Anthony J.} and Isaac, {R. Elwyn}",
year = "2009",
month = mar,
doi = "10.1016/j.peptides.2008.10.020",
language = "English",
volume = "30",
pages = "571--574",
journal = "Peptides",
issn = "0196-9781",
publisher = "Elsevier Inc.",
number = "3",

}

RIS

TY - JOUR

T1 - Locomotor and geotactic behavior of Drosophila melanogaster over-expressing neprilysin 2

AU - Bland, Nicholas D.

AU - Robinson, Philip

AU - Thomas, Josie E.

AU - Shirras, Alan D.

AU - Turner, Anthony J.

AU - Isaac, R. Elwyn

PY - 2009/3

Y1 - 2009/3

N2 - The neprilysin (M13) family of zinc-metallopeptidases has been implicated in a variety of physiological processes, but principally the control of neuropeptide levels in a range of animal species. The overexpression of the amyloid-degrading enzyme, neprilysin, as a therapeutic strategy for Alzheimer's disease is a concept that is gaining in popularity. Here we utilize the GAL4/UAS system to over-express the Drosophila melanogaster Nep2 gene, a close homologue of neprilysin, in flies yielding an increase in NEP2 protein that is detectable by both immunoblotting and enzyme activity. This increase in NEP2 caused a behavioral phenotype manifested in abnormal climbing behavior. Wild type flies climb in a linear, vertical path, but NEP2 over-expressing (Nep2(OEX)) flies tend to climb in a spiral pattern and display an increase in grooming behavior during frequent stationary periods. Nep2(OEX) flies also perform poorly in a geotaxis maze, taking ten times as long to complete the course compared to wild type Drosophila. We hypothesize that the poor performance of the Nep2(OEX) flies in locomotor assays is due to perturbation of neuropeptide signaling and provides evidence of detrimental effects of neprilysin over-expression. (c) 2008 Elsevier Inc. All rights reserved.

AB - The neprilysin (M13) family of zinc-metallopeptidases has been implicated in a variety of physiological processes, but principally the control of neuropeptide levels in a range of animal species. The overexpression of the amyloid-degrading enzyme, neprilysin, as a therapeutic strategy for Alzheimer's disease is a concept that is gaining in popularity. Here we utilize the GAL4/UAS system to over-express the Drosophila melanogaster Nep2 gene, a close homologue of neprilysin, in flies yielding an increase in NEP2 protein that is detectable by both immunoblotting and enzyme activity. This increase in NEP2 caused a behavioral phenotype manifested in abnormal climbing behavior. Wild type flies climb in a linear, vertical path, but NEP2 over-expressing (Nep2(OEX)) flies tend to climb in a spiral pattern and display an increase in grooming behavior during frequent stationary periods. Nep2(OEX) flies also perform poorly in a geotaxis maze, taking ten times as long to complete the course compared to wild type Drosophila. We hypothesize that the poor performance of the Nep2(OEX) flies in locomotor assays is due to perturbation of neuropeptide signaling and provides evidence of detrimental effects of neprilysin over-expression. (c) 2008 Elsevier Inc. All rights reserved.

KW - Drosophila melanogaster

KW - Neprilysin

KW - Alzheimer's disease

KW - Insect behavior

KW - Neuropeptide metabolism

KW - ENDOTHELIN-CONVERTING ENZYME

KW - AMYLOID-BETA PEPTIDE

KW - ALZHEIMERS-DISEASE

KW - THERAPEUTIC TARGETS

KW - A-BETA

KW - MODEL

KW - ENDOPEPTIDASE

KW - DEGRADATION

KW - PATHOLOGY

KW - TOXICITY

U2 - 10.1016/j.peptides.2008.10.020

DO - 10.1016/j.peptides.2008.10.020

M3 - Journal article

VL - 30

SP - 571

EP - 574

JO - Peptides

JF - Peptides

SN - 0196-9781

IS - 3

ER -