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Low dose treatment with polybrominated diphenyl ethers (PBDEs) induce altered characteristics in MCF-7 cells.

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Low dose treatment with polybrominated diphenyl ethers (PBDEs) induce altered characteristics in MCF-7 cells. / Barber, Jonathan L; Walsh, Michael J.; Hewitt, Rebecca et al.
In: Mutagenesis, Vol. 21, No. 5, 21.09.2006, p. 351-360.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

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Barber JL, Walsh MJ, Hewitt R, Jones KC, Martin FL. Low dose treatment with polybrominated diphenyl ethers (PBDEs) induce altered characteristics in MCF-7 cells. Mutagenesis. 2006 Sept 21;21(5):351-360. doi: 10.1093/mutage/gel038

Author

Barber, Jonathan L ; Walsh, Michael J. ; Hewitt, Rebecca et al. / Low dose treatment with polybrominated diphenyl ethers (PBDEs) induce altered characteristics in MCF-7 cells. In: Mutagenesis. 2006 ; Vol. 21, No. 5. pp. 351-360.

Bibtex

@article{579139b32abd48bd86d1eafa2ef0f388,
title = "Low dose treatment with polybrominated diphenyl ethers (PBDEs) induce altered characteristics in MCF-7 cells.",
abstract = "Polybrominated diphenyl ethers (PBDEs) are hydrophobic and persistent additive flame retardants that seemingly transfer into environmental compartments where they bioaccumulate i.e. in human biota. We examined the micronucleus-forming activities of low-dose PBDEs (congeners 47, 99, 153, 183 or 209) in MCF-7 cells along with their ability to modulate growth, cell biochemistry [by infrared (IR) microspectroscopy], clonogenic survival or quantitative expression of cytochrome P450 isoenzymes (CYP1A1, CYP1A2 and CYP1B1), cyclin-dependent kinase inhibitor 1A [CDKN1A (P21WAF1/CIP1)], B-cell leukaemia/lymphoma-2 (BCL-2) and Bcl-2-associated X (BAX). Elevations in micronucleus formation were observed following treatment with 10–12 to 10–9 M PBDE concentrations despite the fact that less than one-fourth of the concentration of each test agent administered partitioned out of the media and into the incubating cells. However, low-dose treatment levels remained within the range of reported concentrations measured in UK serum samples collected in 2003. Clonogenic survival and gene expression was unaltered following 10–12 to 10–9 M PBDE treatment but significant (P < 0.05) elevations in growth kinetics were observed. Significant alterations in IR cell spectra were associated with treatments, and plotted clusters following principal component analysis highlighted these changes. Whether such in vitro effects point to an underlying ability of PBDEs to initiate and drive target-cell alterations in vivo now needs to be addressed.",
author = "Barber, {Jonathan L} and Walsh, {Michael J.} and Rebecca Hewitt and Jones, {Kevin C.} and Martin, {Francis L.}",
year = "2006",
month = sep,
day = "21",
doi = "10.1093/mutage/gel038",
language = "English",
volume = "21",
pages = "351--360",
journal = "Mutagenesis",
issn = "1464-3804",
publisher = "OXFORD UNIV PRESS",
number = "5",

}

RIS

TY - JOUR

T1 - Low dose treatment with polybrominated diphenyl ethers (PBDEs) induce altered characteristics in MCF-7 cells.

AU - Barber, Jonathan L

AU - Walsh, Michael J.

AU - Hewitt, Rebecca

AU - Jones, Kevin C.

AU - Martin, Francis L.

PY - 2006/9/21

Y1 - 2006/9/21

N2 - Polybrominated diphenyl ethers (PBDEs) are hydrophobic and persistent additive flame retardants that seemingly transfer into environmental compartments where they bioaccumulate i.e. in human biota. We examined the micronucleus-forming activities of low-dose PBDEs (congeners 47, 99, 153, 183 or 209) in MCF-7 cells along with their ability to modulate growth, cell biochemistry [by infrared (IR) microspectroscopy], clonogenic survival or quantitative expression of cytochrome P450 isoenzymes (CYP1A1, CYP1A2 and CYP1B1), cyclin-dependent kinase inhibitor 1A [CDKN1A (P21WAF1/CIP1)], B-cell leukaemia/lymphoma-2 (BCL-2) and Bcl-2-associated X (BAX). Elevations in micronucleus formation were observed following treatment with 10–12 to 10–9 M PBDE concentrations despite the fact that less than one-fourth of the concentration of each test agent administered partitioned out of the media and into the incubating cells. However, low-dose treatment levels remained within the range of reported concentrations measured in UK serum samples collected in 2003. Clonogenic survival and gene expression was unaltered following 10–12 to 10–9 M PBDE treatment but significant (P < 0.05) elevations in growth kinetics were observed. Significant alterations in IR cell spectra were associated with treatments, and plotted clusters following principal component analysis highlighted these changes. Whether such in vitro effects point to an underlying ability of PBDEs to initiate and drive target-cell alterations in vivo now needs to be addressed.

AB - Polybrominated diphenyl ethers (PBDEs) are hydrophobic and persistent additive flame retardants that seemingly transfer into environmental compartments where they bioaccumulate i.e. in human biota. We examined the micronucleus-forming activities of low-dose PBDEs (congeners 47, 99, 153, 183 or 209) in MCF-7 cells along with their ability to modulate growth, cell biochemistry [by infrared (IR) microspectroscopy], clonogenic survival or quantitative expression of cytochrome P450 isoenzymes (CYP1A1, CYP1A2 and CYP1B1), cyclin-dependent kinase inhibitor 1A [CDKN1A (P21WAF1/CIP1)], B-cell leukaemia/lymphoma-2 (BCL-2) and Bcl-2-associated X (BAX). Elevations in micronucleus formation were observed following treatment with 10–12 to 10–9 M PBDE concentrations despite the fact that less than one-fourth of the concentration of each test agent administered partitioned out of the media and into the incubating cells. However, low-dose treatment levels remained within the range of reported concentrations measured in UK serum samples collected in 2003. Clonogenic survival and gene expression was unaltered following 10–12 to 10–9 M PBDE treatment but significant (P < 0.05) elevations in growth kinetics were observed. Significant alterations in IR cell spectra were associated with treatments, and plotted clusters following principal component analysis highlighted these changes. Whether such in vitro effects point to an underlying ability of PBDEs to initiate and drive target-cell alterations in vivo now needs to be addressed.

U2 - 10.1093/mutage/gel038

DO - 10.1093/mutage/gel038

M3 - Journal article

VL - 21

SP - 351

EP - 360

JO - Mutagenesis

JF - Mutagenesis

SN - 1464-3804

IS - 5

ER -