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Measurement of cytosolic-free Ca2+ in plant tissue

Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSNChapter

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Standard

Measurement of cytosolic-free Ca2+ in plant tissue. / McAinsh, Martin; Ng, Carl K-Y.
Calcium signalling protocols. ed. / David Lambert; Richard Rainbow. 3rd. ed. USA: Humana Press, 2013. p. 327-341 (Methods in Molecular Biology; Vol. 937).

Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSNChapter

Harvard

McAinsh, M & Ng, CK-Y 2013, Measurement of cytosolic-free Ca2+ in plant tissue. in D Lambert & R Rainbow (eds), Calcium signalling protocols. 3rd edn, Methods in Molecular Biology, vol. 937, Humana Press, USA, pp. 327-341. https://doi.org/10.1007/978-1-62703-086-1_20

APA

McAinsh, M., & Ng, C. K-Y. (2013). Measurement of cytosolic-free Ca2+ in plant tissue. In D. Lambert, & R. Rainbow (Eds.), Calcium signalling protocols (3rd ed., pp. 327-341). (Methods in Molecular Biology; Vol. 937). Humana Press. https://doi.org/10.1007/978-1-62703-086-1_20

Vancouver

McAinsh M, Ng CK-Y. Measurement of cytosolic-free Ca2+ in plant tissue. In Lambert D, Rainbow R, editors, Calcium signalling protocols. 3rd ed. USA: Humana Press. 2013. p. 327-341. (Methods in Molecular Biology). doi: 10.1007/978-1-62703-086-1_20

Author

McAinsh, Martin ; Ng, Carl K-Y. / Measurement of cytosolic-free Ca2+ in plant tissue. Calcium signalling protocols. editor / David Lambert ; Richard Rainbow. 3rd. ed. USA : Humana Press, 2013. pp. 327-341 (Methods in Molecular Biology).

Bibtex

@inbook{6d58b889f6754999b75d91a9ae551957,
title = "Measurement of cytosolic-free Ca2+ in plant tissue",
abstract = "A range of techniques have been used to measure the concentration of cytosolic-free Ca2+ ([Ca2+]cyt) in plant cells. Fluorescent Ca2+-sensitive indicators have been used extensively to measure plant [Ca2+]cyt and a number of techniques are available for loading these into plant cells. Here we describe a method for measuring [Ca2+)cyt in the guard cells of the model plant species Commelina communis by ratio photometry and imaging techniques using the ratiometric fluorescent Ca2+-sensitive indicator fura-2",
keywords = "Commelina communis, guard cell, fura-2, microinjection, ratiometric measurements",
author = "Martin McAinsh and Ng, {Carl K-Y.}",
year = "2013",
doi = "10.1007/978-1-62703-086-1_20",
language = "English",
isbn = "978-1-58829-442-5",
series = "Methods in Molecular Biology",
publisher = "Humana Press",
pages = "327--341",
editor = "David Lambert and Richard Rainbow",
booktitle = "Calcium signalling protocols",
edition = "3rd",

}

RIS

TY - CHAP

T1 - Measurement of cytosolic-free Ca2+ in plant tissue

AU - McAinsh, Martin

AU - Ng, Carl K-Y.

PY - 2013

Y1 - 2013

N2 - A range of techniques have been used to measure the concentration of cytosolic-free Ca2+ ([Ca2+]cyt) in plant cells. Fluorescent Ca2+-sensitive indicators have been used extensively to measure plant [Ca2+]cyt and a number of techniques are available for loading these into plant cells. Here we describe a method for measuring [Ca2+)cyt in the guard cells of the model plant species Commelina communis by ratio photometry and imaging techniques using the ratiometric fluorescent Ca2+-sensitive indicator fura-2

AB - A range of techniques have been used to measure the concentration of cytosolic-free Ca2+ ([Ca2+]cyt) in plant cells. Fluorescent Ca2+-sensitive indicators have been used extensively to measure plant [Ca2+]cyt and a number of techniques are available for loading these into plant cells. Here we describe a method for measuring [Ca2+)cyt in the guard cells of the model plant species Commelina communis by ratio photometry and imaging techniques using the ratiometric fluorescent Ca2+-sensitive indicator fura-2

KW - Commelina communis

KW - guard cell

KW - fura-2

KW - microinjection

KW - ratiometric measurements

U2 - 10.1007/978-1-62703-086-1_20

DO - 10.1007/978-1-62703-086-1_20

M3 - Chapter

SN - 978-1-58829-442-5

T3 - Methods in Molecular Biology

SP - 327

EP - 341

BT - Calcium signalling protocols

A2 - Lambert, David

A2 - Rainbow, Richard

PB - Humana Press

CY - USA

ER -