Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSN › Chapter
Research output: Contribution in Book/Report/Proceedings - With ISBN/ISSN › Chapter
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TY - CHAP
T1 - Measurement of cytosolic-free Ca2+ in plant tissue
AU - McAinsh, Martin
AU - Ng, Carl K-Y.
PY - 2013
Y1 - 2013
N2 - A range of techniques have been used to measure the concentration of cytosolic-free Ca2+ ([Ca2+]cyt) in plant cells. Fluorescent Ca2+-sensitive indicators have been used extensively to measure plant [Ca2+]cyt and a number of techniques are available for loading these into plant cells. Here we describe a method for measuring [Ca2+)cyt in the guard cells of the model plant species Commelina communis by ratio photometry and imaging techniques using the ratiometric fluorescent Ca2+-sensitive indicator fura-2
AB - A range of techniques have been used to measure the concentration of cytosolic-free Ca2+ ([Ca2+]cyt) in plant cells. Fluorescent Ca2+-sensitive indicators have been used extensively to measure plant [Ca2+]cyt and a number of techniques are available for loading these into plant cells. Here we describe a method for measuring [Ca2+)cyt in the guard cells of the model plant species Commelina communis by ratio photometry and imaging techniques using the ratiometric fluorescent Ca2+-sensitive indicator fura-2
KW - Commelina communis
KW - guard cell
KW - fura-2
KW - microinjection
KW - ratiometric measurements
U2 - 10.1007/978-1-62703-086-1_20
DO - 10.1007/978-1-62703-086-1_20
M3 - Chapter
SN - 978-1-58829-442-5
T3 - Methods in Molecular Biology
SP - 327
EP - 341
BT - Calcium signalling protocols
A2 - Lambert, David
A2 - Rainbow, Richard
PB - Humana Press
CY - USA
ER -