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Microspectroscopy of spectral biomarkers associated with human corneal stem cells

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Microspectroscopy of spectral biomarkers associated with human corneal stem cells. / Nakamura, Takahiro; Kerns, Jemma; Trevisan, Julio et al.
In: Molecular Vision, Vol. 16, No. 42, 06.03.2010, p. 359-368.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Nakamura, T, Kerns, J, Trevisan, J, Cooper, LJ, Bentley, A, Carmichael, PL, Scott, AD, Cotte, M, Susini, J, Martin-Hirsch, PL, Kinoshita, S, Fullwood, NJ & Martin, F 2010, 'Microspectroscopy of spectral biomarkers associated with human corneal stem cells', Molecular Vision, vol. 16, no. 42, pp. 359-368. <http://www.molvis.org/molvis/v16/a42/>

APA

Nakamura, T., Kerns, J., Trevisan, J., Cooper, L. J., Bentley, A., Carmichael, P. L., Scott, A. D., Cotte, M., Susini, J., Martin-Hirsch, P. L., Kinoshita, S., Fullwood, N. J., & Martin, F. (2010). Microspectroscopy of spectral biomarkers associated with human corneal stem cells. Molecular Vision, 16(42), 359-368. http://www.molvis.org/molvis/v16/a42/

Vancouver

Nakamura T, Kerns J, Trevisan J, Cooper LJ, Bentley A, Carmichael PL et al. Microspectroscopy of spectral biomarkers associated with human corneal stem cells. Molecular Vision. 2010 Mar 6;16(42):359-368.

Author

Nakamura, Takahiro ; Kerns, Jemma ; Trevisan, Julio et al. / Microspectroscopy of spectral biomarkers associated with human corneal stem cells. In: Molecular Vision. 2010 ; Vol. 16, No. 42. pp. 359-368.

Bibtex

@article{0e2c4707401e40edb3aaeec76f2fddf5,
title = "Microspectroscopy of spectral biomarkers associated with human corneal stem cells",
abstract = "Purpose: Synchrotron-based radiation (SRS) Fourier-transform infrared (FTIR) microspectroscopy potentially provides novel biomarkers of the cell differentiation process. Because such imaging gives a {"}biochemical-cell fingerprint{"} through a cell-sized aperture, we set out to determine whether distinguishing chemical entities associated with putative stem cells (SCs), transit-amplifying (TA) cells, or terminally-differentiated (TD) cells could be identified in human corneal epithelium.Methods: Desiccated cryosections (10 mu m thick) of cornea on barium fluoride infrared transparent windows were interrogated using SRS FTIR microspectroscopy. Infrared analysis was performed through the acquisition of point spectra or image maps.Results: Point spectra were subjected to principal component analysis (PCA) to identify distinguishing chemical entities. Spectral image maps to highlight SCs, TA cells, and TD cells of the cornea were then generated. Point spectrum analysis using PCA highlighted remarkable segregation between the three cell classes. Discriminating chemical entities were associated with several spectral differences over the DNA/RNA (1,425-900 cm(-1)) and protein/lipid (1,800-1480 cm(-1)) regions. Prominent biomarkers of SCs compared to TA cells and/or TD cells were 1,040 cm(-1), 1,080 cm(-1), 1,107 cm(-1), 1,225 cm(-1), 1,400 cm(-1), 1,525 cm(-1), 1,558 cm(-1), and 1,728 cm(-1). Chemical entities associated with DNA/RNA conformation (1,080 cm(-1) and 1,225 cm(-1)) were associated with SCs, whereas protein/lipid biochemicals (1,558 cm(-1) and 1,728 cm(-1)) most distinguished TA cells and TD cells.Conclusions: SRS FTIR microspectroscopy can be employed to identify differential spectral biomarkers of SCs, TA cells, and/or TD cells in human cornea. This nondestructive imaging technology is a novel approach to characterizing SCs in situ.",
keywords = "TRANSFORM INFRARED MICROSPECTROSCOPY, MULTIVARIATE-ANALYSIS, SYNCHROTRON-RADIATION, STEM/PROGENITOR CELLS, AMNIOTIC MEMBRANE, EPITHELIAL-CELLS, SPECTROSCOPY, TRANSPLANTATION, CULTURE, CANCER",
author = "Takahiro Nakamura and Jemma Kerns and Julio Trevisan and Cooper, {Leanne J.} and Adam Bentley and Carmichael, {Paul L.} and Scott, {Andrew D.} and Marine Cotte and Jean Susini and Martin-Hirsch, {Pierre L.} and Shigeru Kinoshita and Fullwood, {Nigel J.} and Frank Martin",
year = "2010",
month = mar,
day = "6",
language = "English",
volume = "16",
pages = "359--368",
journal = "Molecular Vision",
issn = "1090-0535",
publisher = "Molecular Vision",
number = "42",

}

RIS

TY - JOUR

T1 - Microspectroscopy of spectral biomarkers associated with human corneal stem cells

AU - Nakamura, Takahiro

AU - Kerns, Jemma

AU - Trevisan, Julio

AU - Cooper, Leanne J.

AU - Bentley, Adam

AU - Carmichael, Paul L.

AU - Scott, Andrew D.

AU - Cotte, Marine

AU - Susini, Jean

AU - Martin-Hirsch, Pierre L.

AU - Kinoshita, Shigeru

AU - Fullwood, Nigel J.

AU - Martin, Frank

PY - 2010/3/6

Y1 - 2010/3/6

N2 - Purpose: Synchrotron-based radiation (SRS) Fourier-transform infrared (FTIR) microspectroscopy potentially provides novel biomarkers of the cell differentiation process. Because such imaging gives a "biochemical-cell fingerprint" through a cell-sized aperture, we set out to determine whether distinguishing chemical entities associated with putative stem cells (SCs), transit-amplifying (TA) cells, or terminally-differentiated (TD) cells could be identified in human corneal epithelium.Methods: Desiccated cryosections (10 mu m thick) of cornea on barium fluoride infrared transparent windows were interrogated using SRS FTIR microspectroscopy. Infrared analysis was performed through the acquisition of point spectra or image maps.Results: Point spectra were subjected to principal component analysis (PCA) to identify distinguishing chemical entities. Spectral image maps to highlight SCs, TA cells, and TD cells of the cornea were then generated. Point spectrum analysis using PCA highlighted remarkable segregation between the three cell classes. Discriminating chemical entities were associated with several spectral differences over the DNA/RNA (1,425-900 cm(-1)) and protein/lipid (1,800-1480 cm(-1)) regions. Prominent biomarkers of SCs compared to TA cells and/or TD cells were 1,040 cm(-1), 1,080 cm(-1), 1,107 cm(-1), 1,225 cm(-1), 1,400 cm(-1), 1,525 cm(-1), 1,558 cm(-1), and 1,728 cm(-1). Chemical entities associated with DNA/RNA conformation (1,080 cm(-1) and 1,225 cm(-1)) were associated with SCs, whereas protein/lipid biochemicals (1,558 cm(-1) and 1,728 cm(-1)) most distinguished TA cells and TD cells.Conclusions: SRS FTIR microspectroscopy can be employed to identify differential spectral biomarkers of SCs, TA cells, and/or TD cells in human cornea. This nondestructive imaging technology is a novel approach to characterizing SCs in situ.

AB - Purpose: Synchrotron-based radiation (SRS) Fourier-transform infrared (FTIR) microspectroscopy potentially provides novel biomarkers of the cell differentiation process. Because such imaging gives a "biochemical-cell fingerprint" through a cell-sized aperture, we set out to determine whether distinguishing chemical entities associated with putative stem cells (SCs), transit-amplifying (TA) cells, or terminally-differentiated (TD) cells could be identified in human corneal epithelium.Methods: Desiccated cryosections (10 mu m thick) of cornea on barium fluoride infrared transparent windows were interrogated using SRS FTIR microspectroscopy. Infrared analysis was performed through the acquisition of point spectra or image maps.Results: Point spectra were subjected to principal component analysis (PCA) to identify distinguishing chemical entities. Spectral image maps to highlight SCs, TA cells, and TD cells of the cornea were then generated. Point spectrum analysis using PCA highlighted remarkable segregation between the three cell classes. Discriminating chemical entities were associated with several spectral differences over the DNA/RNA (1,425-900 cm(-1)) and protein/lipid (1,800-1480 cm(-1)) regions. Prominent biomarkers of SCs compared to TA cells and/or TD cells were 1,040 cm(-1), 1,080 cm(-1), 1,107 cm(-1), 1,225 cm(-1), 1,400 cm(-1), 1,525 cm(-1), 1,558 cm(-1), and 1,728 cm(-1). Chemical entities associated with DNA/RNA conformation (1,080 cm(-1) and 1,225 cm(-1)) were associated with SCs, whereas protein/lipid biochemicals (1,558 cm(-1) and 1,728 cm(-1)) most distinguished TA cells and TD cells.Conclusions: SRS FTIR microspectroscopy can be employed to identify differential spectral biomarkers of SCs, TA cells, and/or TD cells in human cornea. This nondestructive imaging technology is a novel approach to characterizing SCs in situ.

KW - TRANSFORM INFRARED MICROSPECTROSCOPY

KW - MULTIVARIATE-ANALYSIS

KW - SYNCHROTRON-RADIATION

KW - STEM/PROGENITOR CELLS

KW - AMNIOTIC MEMBRANE

KW - EPITHELIAL-CELLS

KW - SPECTROSCOPY

KW - TRANSPLANTATION

KW - CULTURE

KW - CANCER

M3 - Journal article

C2 - 20520745

VL - 16

SP - 359

EP - 368

JO - Molecular Vision

JF - Molecular Vision

SN - 1090-0535

IS - 42

ER -