Rights statement: This is the peer reviewed version of the following article: Lopez‐Heredia MA, Łapa A, Reczyńska K, et al. Mineralization of gellan gum hydrogels with calcium and magnesium carbonates by alternate soaking in solutions of calcium/magnesium and carbonate ion solutions. J Tissue Eng Regen Med. 2018;12:1825–1834. https://doi.org/10.1002/term.2675 which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/term.2675 This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Mineralization of gellan gum hydrogels with calcium and magnesium carbonates by alternate soaking for bone regeneration applications
AU - Lopez-Heredia, Marco
AU - Lapa, Agata
AU - Reczynska, Katarzyna
AU - Pietryga, Krzysztof
AU - Balcaen, Lieve
AU - Mendes, Ana
AU - Schaubroeck, David
AU - Van der Voort, Pascal
AU - Dokupil, Agnieszka
AU - Plis, Agnieszka
AU - Stevens, Chris
AU - Parakhonskiy, Bogdan
AU - Samal, Sangram
AU - Vanhaecke, Frank
AU - Chai, Feng
AU - Chronakis, Ioannis
AU - Blanchemain, Nicolas
AU - Pamula, Elzbieta
AU - Skirtach, Andre G.
AU - Douglas, Timothy Edward Lim
N1 - This is the peer reviewed version of the following article: Lopez‐Heredia MA, Łapa A, Reczyńska K, et al. Mineralization of gellan gum hydrogels with calcium and magnesium carbonates by alternate soaking in solutions of calcium/magnesium and carbonate ion solutions. J Tissue Eng Regen Med. 2018;12:1825–1834. https://doi.org/10.1002/term.2675 which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/term.2675 This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
PY - 2018/8
Y1 - 2018/8
N2 - Mineralization of hydrogels is desirable prior to applications in bone regeneration. CaCO3 is a widely used bone regeneration material and Mg, when used as a component of calcium phosphate biomaterials, has promoted bone‐forming cell adhesion and proliferation and bone regeneration. In this study, gellan gum (GG) hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution. This alternate soaking cycle was repeated five times. Five different calcium and/or magnesium ion solutions, containing different molar ratios of Ca to Mg ranging from Mg‐free to Ca‐free were compared. Carbonate mineral formed in all sample groups subjected to the Ca:Mg elemental ratio in the carbonate mineral formed was higher than in the respective mineralizing solution. Mineral formed in the absence of Mg was predominantly CaCO3 in the form of a mixture of calcite and vaterite. Increasing the Mg content in the mineral formed led to the formation of magnesian calcite, decreased the total amount of the mineral formed and its crystallinity. Hydrogel mineralization and increasing Mg content in mineral formed did not obviously improve proliferation of MC3T3‐E1 osteoblast‐like cells or differentiation after 7 days.
AB - Mineralization of hydrogels is desirable prior to applications in bone regeneration. CaCO3 is a widely used bone regeneration material and Mg, when used as a component of calcium phosphate biomaterials, has promoted bone‐forming cell adhesion and proliferation and bone regeneration. In this study, gellan gum (GG) hydrogels were mineralized with carbonates containing different amounts of calcium (Ca) and magnesium (Mg) by alternate soaking in, firstly, a calcium and/or magnesium ion solution and, secondly, a carbonate ion solution. This alternate soaking cycle was repeated five times. Five different calcium and/or magnesium ion solutions, containing different molar ratios of Ca to Mg ranging from Mg‐free to Ca‐free were compared. Carbonate mineral formed in all sample groups subjected to the Ca:Mg elemental ratio in the carbonate mineral formed was higher than in the respective mineralizing solution. Mineral formed in the absence of Mg was predominantly CaCO3 in the form of a mixture of calcite and vaterite. Increasing the Mg content in the mineral formed led to the formation of magnesian calcite, decreased the total amount of the mineral formed and its crystallinity. Hydrogel mineralization and increasing Mg content in mineral formed did not obviously improve proliferation of MC3T3‐E1 osteoblast‐like cells or differentiation after 7 days.
KW - Gellan gum hydrogels
KW - calcium carbonate
KW - magnesium
KW - mineralization
KW - composite
U2 - 10.1002/term.2675
DO - 10.1002/term.2675
M3 - Journal article
VL - 12
SP - 1825
EP - 1834
JO - Journal of Tissue Engineering and Regenerative Medicine
JF - Journal of Tissue Engineering and Regenerative Medicine
SN - 1932-6254
IS - 8
ER -