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Molecular characterization of infectious bursal disease viruses from Pakistan

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Molecular characterization of infectious bursal disease viruses from Pakistan. / Shabbir, Muhammad Zubair; Ali, Muhammad; Abbas, Muhammad et al.
In: Archives of Virology, Vol. 161, No. 7, 07.2016, p. 2001-2006.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Shabbir, MZ, Ali, M, Abbas, M, Chaudhry, UN, Zia-Ur-Rehman, & Munir, M 2016, 'Molecular characterization of infectious bursal disease viruses from Pakistan', Archives of Virology, vol. 161, no. 7, pp. 2001-2006. https://doi.org/10.1007/s00705-016-2869-9

APA

Shabbir, M. Z., Ali, M., Abbas, M., Chaudhry, U. N., Zia-Ur-Rehman, & Munir, M. (2016). Molecular characterization of infectious bursal disease viruses from Pakistan. Archives of Virology, 161(7), 2001-2006. https://doi.org/10.1007/s00705-016-2869-9

Vancouver

Shabbir MZ, Ali M, Abbas M, Chaudhry UN, Zia-Ur-Rehman , Munir M. Molecular characterization of infectious bursal disease viruses from Pakistan. Archives of Virology. 2016 Jul;161(7):2001-2006. Epub 2016 Apr 23. doi: 10.1007/s00705-016-2869-9

Author

Shabbir, Muhammad Zubair ; Ali, Muhammad ; Abbas, Muhammad et al. / Molecular characterization of infectious bursal disease viruses from Pakistan. In: Archives of Virology. 2016 ; Vol. 161, No. 7. pp. 2001-2006.

Bibtex

@article{1ed9604009f644bc980467d025fb1559,
title = "Molecular characterization of infectious bursal disease viruses from Pakistan",
abstract = "Since the first report of infectious bursal disease in Pakistan in 1987, outbreaks have been common even in vaccinated flocks. Despite appropriate administration of vaccines, concerns arise if the circulating strains are different from the ones used in the vaccine. Here, we sequenced the hypervariable region (HVR) of the VP2 gene of circulating strains of infectious bursal disease virus (IBDV) originating from outbreaks (n = 4) in broiler flocks in Pakistan. Nucleotide sequencing followed by phylogeny and deduced amino acid sequence analysis showed the circulating strains to be very virulent (vv) and identified characteristic residues at position 222 (A), 242 (I), 256 (I), 294 (I) and 299 (S). In addition, a substitution at positions 221 (Q→H) was found to be exclusive to Pakistani strains in our analysis, although a larger dataset is required to confirm this finding. Compared to vaccine strains that are commonly used in Pakistan, substitution mutations were found at key amino acid positions in VP2 that may be responsible for potential changes in neutralization epitopes and vaccine failure.",
keywords = "Amino Acid Sequence, Animals, Birnaviridae Infections, Capsid Proteins, Chickens, Disease Outbreaks, Infectious bursal disease virus, Molecular Sequence Data, Pakistan, Phylogeny, Poultry Diseases, Sequence Alignment, Journal Article",
author = "Shabbir, {Muhammad Zubair} and Muhammad Ali and Muhammad Abbas and Chaudhry, {Umer Naveed} and Zia-Ur-Rehman and Muhammad Munir",
year = "2016",
month = jul,
doi = "10.1007/s00705-016-2869-9",
language = "English",
volume = "161",
pages = "2001--2006",
journal = "Archives of Virology",
issn = "0304-8608",
publisher = "Springer-Verlag Wien",
number = "7",

}

RIS

TY - JOUR

T1 - Molecular characterization of infectious bursal disease viruses from Pakistan

AU - Shabbir, Muhammad Zubair

AU - Ali, Muhammad

AU - Abbas, Muhammad

AU - Chaudhry, Umer Naveed

AU - Zia-Ur-Rehman, null

AU - Munir, Muhammad

PY - 2016/7

Y1 - 2016/7

N2 - Since the first report of infectious bursal disease in Pakistan in 1987, outbreaks have been common even in vaccinated flocks. Despite appropriate administration of vaccines, concerns arise if the circulating strains are different from the ones used in the vaccine. Here, we sequenced the hypervariable region (HVR) of the VP2 gene of circulating strains of infectious bursal disease virus (IBDV) originating from outbreaks (n = 4) in broiler flocks in Pakistan. Nucleotide sequencing followed by phylogeny and deduced amino acid sequence analysis showed the circulating strains to be very virulent (vv) and identified characteristic residues at position 222 (A), 242 (I), 256 (I), 294 (I) and 299 (S). In addition, a substitution at positions 221 (Q→H) was found to be exclusive to Pakistani strains in our analysis, although a larger dataset is required to confirm this finding. Compared to vaccine strains that are commonly used in Pakistan, substitution mutations were found at key amino acid positions in VP2 that may be responsible for potential changes in neutralization epitopes and vaccine failure.

AB - Since the first report of infectious bursal disease in Pakistan in 1987, outbreaks have been common even in vaccinated flocks. Despite appropriate administration of vaccines, concerns arise if the circulating strains are different from the ones used in the vaccine. Here, we sequenced the hypervariable region (HVR) of the VP2 gene of circulating strains of infectious bursal disease virus (IBDV) originating from outbreaks (n = 4) in broiler flocks in Pakistan. Nucleotide sequencing followed by phylogeny and deduced amino acid sequence analysis showed the circulating strains to be very virulent (vv) and identified characteristic residues at position 222 (A), 242 (I), 256 (I), 294 (I) and 299 (S). In addition, a substitution at positions 221 (Q→H) was found to be exclusive to Pakistani strains in our analysis, although a larger dataset is required to confirm this finding. Compared to vaccine strains that are commonly used in Pakistan, substitution mutations were found at key amino acid positions in VP2 that may be responsible for potential changes in neutralization epitopes and vaccine failure.

KW - Amino Acid Sequence

KW - Animals

KW - Birnaviridae Infections

KW - Capsid Proteins

KW - Chickens

KW - Disease Outbreaks

KW - Infectious bursal disease virus

KW - Molecular Sequence Data

KW - Pakistan

KW - Phylogeny

KW - Poultry Diseases

KW - Sequence Alignment

KW - Journal Article

U2 - 10.1007/s00705-016-2869-9

DO - 10.1007/s00705-016-2869-9

M3 - Journal article

C2 - 27107876

VL - 161

SP - 2001

EP - 2006

JO - Archives of Virology

JF - Archives of Virology

SN - 0304-8608

IS - 7

ER -