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Morphological transformation of C3H/M2 mouse fibroblasts by, and genotoxicity of, extracts of human milk.

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Morphological transformation of C3H/M2 mouse fibroblasts by, and genotoxicity of, extracts of human milk. / Pfau, Wolfgang; Martin, Francis L.; Cole, Kathleen J. et al.
In: Mutation Research/Genetic Toxicology and Environmental Mutagenesis, Vol. 498, No. 1-2, 15.11.2001, p. 207-217.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Pfau, W, Martin, FL, Cole, KJ, Weaver, G, Marquardt, H, Phillips, DH & Grover, PL 2001, 'Morphological transformation of C3H/M2 mouse fibroblasts by, and genotoxicity of, extracts of human milk.', Mutation Research/Genetic Toxicology and Environmental Mutagenesis, vol. 498, no. 1-2, pp. 207-217. https://doi.org/10.1016/S1383-5718(01)00284-4

APA

Pfau, W., Martin, F. L., Cole, K. J., Weaver, G., Marquardt, H., Phillips, D. H., & Grover, P. L. (2001). Morphological transformation of C3H/M2 mouse fibroblasts by, and genotoxicity of, extracts of human milk. Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 498(1-2), 207-217. https://doi.org/10.1016/S1383-5718(01)00284-4

Vancouver

Pfau W, Martin FL, Cole KJ, Weaver G, Marquardt H, Phillips DH et al. Morphological transformation of C3H/M2 mouse fibroblasts by, and genotoxicity of, extracts of human milk. Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2001 Nov 15;498(1-2):207-217. doi: 10.1016/S1383-5718(01)00284-4

Author

Pfau, Wolfgang ; Martin, Francis L. ; Cole, Kathleen J. et al. / Morphological transformation of C3H/M2 mouse fibroblasts by, and genotoxicity of, extracts of human milk. In: Mutation Research/Genetic Toxicology and Environmental Mutagenesis. 2001 ; Vol. 498, No. 1-2. pp. 207-217.

Bibtex

@article{611141b8c7a049d7af488a201b859259,
title = "Morphological transformation of C3H/M2 mouse fibroblasts by, and genotoxicity of, extracts of human milk.",
abstract = "Breast cancer may be initiated by environmental/dietary agents and human milk may act as an ex vivo indicator of in vivo exposure of mammary epithelial cells to genotoxins. Extracts of human milk from UK-resident women (n=7) were tested for their abilities to morphologically transform C3H/M2 mouse fibroblasts. Genotoxicities were assessed in the Salmonella typhimurium reverse-mutation assay in the presence of S9 using strains TA1538 and YG1019, and in metabolically-competent human MCL-5 cells with the micronucleus and with the alkaline single cell gel electrophoresis (comet) assays. Two of the seven extracts were inactive in the transformation assay both in the presence or absence of S9, two appeared to be equally transforming either in the presence or absence of S9, and two other extracts induced increased transformation frequencies in the presence of S9. A seventh extract, tested only in the absence of S9, was inactive. Extracts were either active or inactive in at least three of the four tests applied. Four extracts were active or inactive in all four tests. The results suggest that human milk could be used as a resource for investigations of the as-yet-unidentified transforming agents previously detected in mammary lipid.",
keywords = "Morphological transformation, Human milk extracts, Breast cancer, Ames test, Micronucleus assay, Comet assay",
author = "Wolfgang Pfau and Martin, {Francis L.} and Cole, {Kathleen J.} and Gillian Weaver and Hans Marquardt and Phillips, {David H.} and Grover, {Philip L.}",
year = "2001",
month = nov,
day = "15",
doi = "10.1016/S1383-5718(01)00284-4",
language = "English",
volume = "498",
pages = "207--217",
journal = "Mutation Research/Genetic Toxicology and Environmental Mutagenesis",
issn = "1383-5718",
publisher = "Elsevier",
number = "1-2",

}

RIS

TY - JOUR

T1 - Morphological transformation of C3H/M2 mouse fibroblasts by, and genotoxicity of, extracts of human milk.

AU - Pfau, Wolfgang

AU - Martin, Francis L.

AU - Cole, Kathleen J.

AU - Weaver, Gillian

AU - Marquardt, Hans

AU - Phillips, David H.

AU - Grover, Philip L.

PY - 2001/11/15

Y1 - 2001/11/15

N2 - Breast cancer may be initiated by environmental/dietary agents and human milk may act as an ex vivo indicator of in vivo exposure of mammary epithelial cells to genotoxins. Extracts of human milk from UK-resident women (n=7) were tested for their abilities to morphologically transform C3H/M2 mouse fibroblasts. Genotoxicities were assessed in the Salmonella typhimurium reverse-mutation assay in the presence of S9 using strains TA1538 and YG1019, and in metabolically-competent human MCL-5 cells with the micronucleus and with the alkaline single cell gel electrophoresis (comet) assays. Two of the seven extracts were inactive in the transformation assay both in the presence or absence of S9, two appeared to be equally transforming either in the presence or absence of S9, and two other extracts induced increased transformation frequencies in the presence of S9. A seventh extract, tested only in the absence of S9, was inactive. Extracts were either active or inactive in at least three of the four tests applied. Four extracts were active or inactive in all four tests. The results suggest that human milk could be used as a resource for investigations of the as-yet-unidentified transforming agents previously detected in mammary lipid.

AB - Breast cancer may be initiated by environmental/dietary agents and human milk may act as an ex vivo indicator of in vivo exposure of mammary epithelial cells to genotoxins. Extracts of human milk from UK-resident women (n=7) were tested for their abilities to morphologically transform C3H/M2 mouse fibroblasts. Genotoxicities were assessed in the Salmonella typhimurium reverse-mutation assay in the presence of S9 using strains TA1538 and YG1019, and in metabolically-competent human MCL-5 cells with the micronucleus and with the alkaline single cell gel electrophoresis (comet) assays. Two of the seven extracts were inactive in the transformation assay both in the presence or absence of S9, two appeared to be equally transforming either in the presence or absence of S9, and two other extracts induced increased transformation frequencies in the presence of S9. A seventh extract, tested only in the absence of S9, was inactive. Extracts were either active or inactive in at least three of the four tests applied. Four extracts were active or inactive in all four tests. The results suggest that human milk could be used as a resource for investigations of the as-yet-unidentified transforming agents previously detected in mammary lipid.

KW - Morphological transformation

KW - Human milk extracts

KW - Breast cancer

KW - Ames test

KW - Micronucleus assay

KW - Comet assay

U2 - 10.1016/S1383-5718(01)00284-4

DO - 10.1016/S1383-5718(01)00284-4

M3 - Journal article

VL - 498

SP - 207

EP - 217

JO - Mutation Research/Genetic Toxicology and Environmental Mutagenesis

JF - Mutation Research/Genetic Toxicology and Environmental Mutagenesis

SN - 1383-5718

IS - 1-2

ER -