12,000

We have over 12,000 students, from over 100 countries, within one of the safest campuses in the UK

93%

93% of Lancaster students go into work or further study within six months of graduating

Home > Research > Publications & Outputs > Multiple Non-reducing Chain Termini Isolated fr...
View graph of relations

« Back

Multiple Non-reducing Chain Termini Isolated from Bovine Corneal Keratan Sulfates.

Research output: Contribution to journalJournal article

Published

Journal publication date09/1996
JournalJournal of Biological Chemistry
Journal number38
Volume271
Number of pages12
Pages23535-23546
Original languageEnglish

Abstract

From the Division of Biological Sciences, Institute of Environmental and Biological Sciences, Lancaster University, Bailrigg, Lancaster LA1 4YQ and The Polymer Centre, School of Physics and Chemistry, Lancaster University, Bailrigg, Lancaster LA1 4YA, United Kingdom Keratan sulfate-containing proteoglycans were isolated from bovine cornea (15-month-old to 3-year-old animals) and digested with the enzyme, keratanase II. The released oligosaccharides, which included non-reducing termini and repeat region oligosaccharides but not linkage regions, were reduced with alkaline borohydride and fractionated on a Spherisorb column. These oligosaccharides were examined by 600-MHz 1H NMR spectroscopy using one- and two-dimensional methods and, in addition to some oligosaccharide alditols previously recovered from skeletal keratan sulfate, the following new capping structures were identified: NeuAc2-6Gal1-4GlcNAc(S)-ol, NeuAc2-3Gal(S)1-4GlcNAc(S)1-3Gal1-4GlcNAc(S)-ol, NeuGc2-6Gal1-4GlcNAc(S)1-3Gal1-4GlcNAc(S)-ol, NeuGc2-3Gal1-4GlcNAc(S)1-3Gal1-4GlcNAc(S)-ol, NeuGc2-3Gal(S)1-4GlcNAc(S)1-3Gal1-4GlcNAc(S)-ol, NeuGc2-3Gal(S)1-4GlcNAc(S)1-3Gal(S)1-4GlcNAc(S)-ol, Gal1-3Gal1-4GlcNAc(S)1-3Gal1-4GlcNAc(S)-ol, Gal1-3Gal1-4GlcNAc(S)1-3Gal(S)1-4GlcNAc(S)-ol, GlcNAc(S)1-3Gal(S)1-4GlcNAc(S)-ol, and GalNAc(S)1-3Gal(S)1-4GlcNAc(S)-ol. These structures represent seven families of capping residues, whose relative molar proportions are given in parentheses: NeuAc(2-3)- (12%), NeuAc(2-6)- (41%), NeuGc(2-3)- and NeuGc(2-6)- families (12%), Gal(1-3)- (26%), GalNAc(S)(1-3)- (5%), and GlcNAc(S)(1-3)- (4%). It is not clear, at present, where each of these structures occurs on the bi-antennary N-linked corneal keratan sulfate chains, which themselves occur within three keratan sulfate proteoglycan species. However, examination of the relative proportions of the capping to the repeat structures and knowledge of the average molecular size suggests that the sum of these non-reducing termini represents the caps of two antennae.