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New label-free automated survival assays reveal unexpected stress resistance patterns during C. elegans aging

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New label-free automated survival assays reveal unexpected stress resistance patterns during C. elegans aging. / Benedetto, Alexandre; Bambade, Timothée; Au, Catherine; Tullet, Jennifer M.A.; Monkhouse, Jennifer; Dang, Hairuo; Cetnar, Kalina; Chan, Brian; Cabreiro, Filipe; Gems, David.

In: Aging Cell, Vol. 18, No. 5, e12998, 01.10.2019.

Research output: Contribution to journalJournal article

Harvard

Benedetto, A, Bambade, T, Au, C, Tullet, JMA, Monkhouse, J, Dang, H, Cetnar, K, Chan, B, Cabreiro, F & Gems, D 2019, 'New label-free automated survival assays reveal unexpected stress resistance patterns during C. elegans aging', Aging Cell, vol. 18, no. 5, e12998. https://doi.org/10.1111/acel.12998

APA

Benedetto, A., Bambade, T., Au, C., Tullet, J. M. A., Monkhouse, J., Dang, H., Cetnar, K., Chan, B., Cabreiro, F., & Gems, D. (2019). New label-free automated survival assays reveal unexpected stress resistance patterns during C. elegans aging. Aging Cell, 18(5), [e12998]. https://doi.org/10.1111/acel.12998

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Author

Benedetto, Alexandre ; Bambade, Timothée ; Au, Catherine ; Tullet, Jennifer M.A. ; Monkhouse, Jennifer ; Dang, Hairuo ; Cetnar, Kalina ; Chan, Brian ; Cabreiro, Filipe ; Gems, David. / New label-free automated survival assays reveal unexpected stress resistance patterns during C. elegans aging. In: Aging Cell. 2019 ; Vol. 18, No. 5.

Bibtex

@article{bc5fc74a3c374f38bb08f593d28d7e34,
title = "New label-free automated survival assays reveal unexpected stress resistance patterns during C. elegans aging",
abstract = "Caenorhabditis elegans is an excellent model for high-throughput experimental approaches but lacks an automated means to pinpoint time of death during survival assays over a short time frame, that is, easy to implement, highly scalable, robust, and versatile. Here, we describe an automated, label-free, high-throughput method using death-associated fluorescence to monitor nematode population survival (dubbed LFASS for label-free automated survival scoring), which we apply to severe stress and infection resistance assays. We demonstrate its use to define correlations between age, longevity, and severe stress resistance, and its applicability to parasitic nematodes. The use of LFASS to assess the effects of aging on susceptibility to severe stress revealed an unexpected increase in stress resistance with advancing age, which was largely autophagy-dependent. Correlation analysis further revealed that while severe thermal stress resistance positively correlates with lifespan, severe oxidative stress resistance does not. This supports the view that temperature-sensitive protein-handling processes more than redox homeostasis underpin aging in C. elegans. That the ages of peak resistance to infection, severe oxidative stress, heat shock, and milder stressors differ markedly suggests that stress resistance and health span do not show a simple correspondence in C. elegans.",
keywords = "aging, autophagy, C. elegans, infection, stress, survival",
author = "Alexandre Benedetto and Timoth{\'e}e Bambade and Catherine Au and Tullet, {Jennifer M.A.} and Jennifer Monkhouse and Hairuo Dang and Kalina Cetnar and Brian Chan and Filipe Cabreiro and David Gems",
year = "2019",
month = oct,
day = "1",
doi = "10.1111/acel.12998",
language = "English",
volume = "18",
journal = "Aging Cell",
issn = "1474-9718",
publisher = "Wiley-Blackwell",
number = "5",

}

RIS

TY - JOUR

T1 - New label-free automated survival assays reveal unexpected stress resistance patterns during C. elegans aging

AU - Benedetto, Alexandre

AU - Bambade, Timothée

AU - Au, Catherine

AU - Tullet, Jennifer M.A.

AU - Monkhouse, Jennifer

AU - Dang, Hairuo

AU - Cetnar, Kalina

AU - Chan, Brian

AU - Cabreiro, Filipe

AU - Gems, David

PY - 2019/10/1

Y1 - 2019/10/1

N2 - Caenorhabditis elegans is an excellent model for high-throughput experimental approaches but lacks an automated means to pinpoint time of death during survival assays over a short time frame, that is, easy to implement, highly scalable, robust, and versatile. Here, we describe an automated, label-free, high-throughput method using death-associated fluorescence to monitor nematode population survival (dubbed LFASS for label-free automated survival scoring), which we apply to severe stress and infection resistance assays. We demonstrate its use to define correlations between age, longevity, and severe stress resistance, and its applicability to parasitic nematodes. The use of LFASS to assess the effects of aging on susceptibility to severe stress revealed an unexpected increase in stress resistance with advancing age, which was largely autophagy-dependent. Correlation analysis further revealed that while severe thermal stress resistance positively correlates with lifespan, severe oxidative stress resistance does not. This supports the view that temperature-sensitive protein-handling processes more than redox homeostasis underpin aging in C. elegans. That the ages of peak resistance to infection, severe oxidative stress, heat shock, and milder stressors differ markedly suggests that stress resistance and health span do not show a simple correspondence in C. elegans.

AB - Caenorhabditis elegans is an excellent model for high-throughput experimental approaches but lacks an automated means to pinpoint time of death during survival assays over a short time frame, that is, easy to implement, highly scalable, robust, and versatile. Here, we describe an automated, label-free, high-throughput method using death-associated fluorescence to monitor nematode population survival (dubbed LFASS for label-free automated survival scoring), which we apply to severe stress and infection resistance assays. We demonstrate its use to define correlations between age, longevity, and severe stress resistance, and its applicability to parasitic nematodes. The use of LFASS to assess the effects of aging on susceptibility to severe stress revealed an unexpected increase in stress resistance with advancing age, which was largely autophagy-dependent. Correlation analysis further revealed that while severe thermal stress resistance positively correlates with lifespan, severe oxidative stress resistance does not. This supports the view that temperature-sensitive protein-handling processes more than redox homeostasis underpin aging in C. elegans. That the ages of peak resistance to infection, severe oxidative stress, heat shock, and milder stressors differ markedly suggests that stress resistance and health span do not show a simple correspondence in C. elegans.

KW - aging

KW - autophagy

KW - C. elegans

KW - infection

KW - stress

KW - survival

U2 - 10.1111/acel.12998

DO - 10.1111/acel.12998

M3 - Journal article

C2 - 31309734

AN - SCOPUS:85071662444

VL - 18

JO - Aging Cell

JF - Aging Cell

SN - 1474-9718

IS - 5

M1 - e12998

ER -