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Nickel elicits a fast antioxidant response in Coffea arabica cells.

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Nickel elicits a fast antioxidant response in Coffea arabica cells. / Gomes-Junior, R. A.; Moldes, C. A.; Gratão, P. L. et al.
In: Plant Physiology and Biochemistry, Vol. 44, No. 5-6, 2006, p. 420-429.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Gomes-Junior, RA, Moldes, CA, Gratão, PL, Mazzafera, P, Lea, PJ & Azevedo, RA 2006, 'Nickel elicits a fast antioxidant response in Coffea arabica cells.', Plant Physiology and Biochemistry, vol. 44, no. 5-6, pp. 420-429. https://doi.org/10.1016/j.plaphy.2006.06.002

APA

Gomes-Junior, R. A., Moldes, C. A., Gratão, P. L., Mazzafera, P., Lea, P. J., & Azevedo, R. A. (2006). Nickel elicits a fast antioxidant response in Coffea arabica cells. Plant Physiology and Biochemistry, 44(5-6), 420-429. https://doi.org/10.1016/j.plaphy.2006.06.002

Vancouver

Gomes-Junior RA, Moldes CA, Gratão PL, Mazzafera P, Lea PJ, Azevedo RA. Nickel elicits a fast antioxidant response in Coffea arabica cells. Plant Physiology and Biochemistry. 2006;44(5-6):420-429. doi: 10.1016/j.plaphy.2006.06.002

Author

Gomes-Junior, R. A. ; Moldes, C. A. ; Gratão, P. L. et al. / Nickel elicits a fast antioxidant response in Coffea arabica cells. In: Plant Physiology and Biochemistry. 2006 ; Vol. 44, No. 5-6. pp. 420-429.

Bibtex

@article{cb3b27ed2a104b4fbae42e3576e21a1b,
title = "Nickel elicits a fast antioxidant response in Coffea arabica cells.",
abstract = "The antioxidant responses of coffee (Coffea arabica L.) cell suspension cultures to nickel (Ni) were investigated. Ni was very rapidly accumulated in the cells and the accumulation could be directly correlated with the increase of NiCl2 concentration in the medium. At 0.05 mM NiCl2 growth was stimulated, but at 0.5 mM NiCl2, the growth rate was reduced. An indication of alterations in the presence of reactive oxygen species was detected by an increase in lipid peroxidation at 0.5 mM NiCl2. Catalase (CAT; EC 1.11.1.6), glutathione reductase (GR; EC 1.6.4.2), ascorbate peroxidase (APX; EC 1.11.1.11), guaiacol peroxidase (GOPX; EC 1.11.1.7) and superoxide dismutase (SOD; EC 1.15.1.1) activities were increased, particularly at earlier NiCl2 exposure times and the activities were higher at 0.5 mM NiCl2 for most of exposure times tested. Non-denaturing PAGE revealed one CAT isoenzyme, nine SOD isoenzymes and four GR isoenzymes. The SOD isoenzymes were differentially affected by NiCl2 treatment and one GR isoenzyme was increased by NiCl2. NiCl2 at 0.05 mM did not induce lipid peroxidation and the main response appeared to be via the induction of SOD, CAT, GOPX and APX activities for the removal of the reactive oxygen species and through the induction of GR to ensure the availability of reduced glutathione.",
keywords = "Coffea arabica, Oxidative stress, Lipid peroxidation, Nickel, Catalase, Glutathione reductase, Superoxide dismutase",
author = "Gomes-Junior, {R. A.} and Moldes, {C. A.} and Grat{\~a}o, {P. L.} and P. Mazzafera and Lea, {P. J.} and Azevedo, {R. A.}",
year = "2006",
doi = "10.1016/j.plaphy.2006.06.002",
language = "English",
volume = "44",
pages = "420--429",
journal = "Plant Physiology and Biochemistry",
issn = "0981-9428",
publisher = "Elsevier Masson SAS",
number = "5-6",

}

RIS

TY - JOUR

T1 - Nickel elicits a fast antioxidant response in Coffea arabica cells.

AU - Gomes-Junior, R. A.

AU - Moldes, C. A.

AU - Gratão, P. L.

AU - Mazzafera, P.

AU - Lea, P. J.

AU - Azevedo, R. A.

PY - 2006

Y1 - 2006

N2 - The antioxidant responses of coffee (Coffea arabica L.) cell suspension cultures to nickel (Ni) were investigated. Ni was very rapidly accumulated in the cells and the accumulation could be directly correlated with the increase of NiCl2 concentration in the medium. At 0.05 mM NiCl2 growth was stimulated, but at 0.5 mM NiCl2, the growth rate was reduced. An indication of alterations in the presence of reactive oxygen species was detected by an increase in lipid peroxidation at 0.5 mM NiCl2. Catalase (CAT; EC 1.11.1.6), glutathione reductase (GR; EC 1.6.4.2), ascorbate peroxidase (APX; EC 1.11.1.11), guaiacol peroxidase (GOPX; EC 1.11.1.7) and superoxide dismutase (SOD; EC 1.15.1.1) activities were increased, particularly at earlier NiCl2 exposure times and the activities were higher at 0.5 mM NiCl2 for most of exposure times tested. Non-denaturing PAGE revealed one CAT isoenzyme, nine SOD isoenzymes and four GR isoenzymes. The SOD isoenzymes were differentially affected by NiCl2 treatment and one GR isoenzyme was increased by NiCl2. NiCl2 at 0.05 mM did not induce lipid peroxidation and the main response appeared to be via the induction of SOD, CAT, GOPX and APX activities for the removal of the reactive oxygen species and through the induction of GR to ensure the availability of reduced glutathione.

AB - The antioxidant responses of coffee (Coffea arabica L.) cell suspension cultures to nickel (Ni) were investigated. Ni was very rapidly accumulated in the cells and the accumulation could be directly correlated with the increase of NiCl2 concentration in the medium. At 0.05 mM NiCl2 growth was stimulated, but at 0.5 mM NiCl2, the growth rate was reduced. An indication of alterations in the presence of reactive oxygen species was detected by an increase in lipid peroxidation at 0.5 mM NiCl2. Catalase (CAT; EC 1.11.1.6), glutathione reductase (GR; EC 1.6.4.2), ascorbate peroxidase (APX; EC 1.11.1.11), guaiacol peroxidase (GOPX; EC 1.11.1.7) and superoxide dismutase (SOD; EC 1.15.1.1) activities were increased, particularly at earlier NiCl2 exposure times and the activities were higher at 0.5 mM NiCl2 for most of exposure times tested. Non-denaturing PAGE revealed one CAT isoenzyme, nine SOD isoenzymes and four GR isoenzymes. The SOD isoenzymes were differentially affected by NiCl2 treatment and one GR isoenzyme was increased by NiCl2. NiCl2 at 0.05 mM did not induce lipid peroxidation and the main response appeared to be via the induction of SOD, CAT, GOPX and APX activities for the removal of the reactive oxygen species and through the induction of GR to ensure the availability of reduced glutathione.

KW - Coffea arabica

KW - Oxidative stress

KW - Lipid peroxidation

KW - Nickel

KW - Catalase

KW - Glutathione reductase

KW - Superoxide dismutase

U2 - 10.1016/j.plaphy.2006.06.002

DO - 10.1016/j.plaphy.2006.06.002

M3 - Journal article

VL - 44

SP - 420

EP - 429

JO - Plant Physiology and Biochemistry

JF - Plant Physiology and Biochemistry

SN - 0981-9428

IS - 5-6

ER -