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Nonsense-mediated mRNA decay in Drosophila: at the intersection of the yeast and mammalian pathways

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Nonsense-mediated mRNA decay in Drosophila: at the intersection of the yeast and mammalian pathways. / Gatfield, David; Unterholzner, Leonie; Ciccarelli, Francesca D et al.
In: EMBO Journal, Vol. 22, No. 15, 01.08.2003, p. 3960-3970.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Gatfield, D, Unterholzner, L, Ciccarelli, FD, Bork, P & Izaurralde, E 2003, 'Nonsense-mediated mRNA decay in Drosophila: at the intersection of the yeast and mammalian pathways', EMBO Journal, vol. 22, no. 15, pp. 3960-3970. https://doi.org/10.1093/emboj/cdg371

APA

Gatfield, D., Unterholzner, L., Ciccarelli, F. D., Bork, P., & Izaurralde, E. (2003). Nonsense-mediated mRNA decay in Drosophila: at the intersection of the yeast and mammalian pathways. EMBO Journal, 22(15), 3960-3970. https://doi.org/10.1093/emboj/cdg371

Vancouver

Gatfield D, Unterholzner L, Ciccarelli FD, Bork P, Izaurralde E. Nonsense-mediated mRNA decay in Drosophila: at the intersection of the yeast and mammalian pathways. EMBO Journal. 2003 Aug 1;22(15):3960-3970. doi: 10.1093/emboj/cdg371

Author

Gatfield, David ; Unterholzner, Leonie ; Ciccarelli, Francesca D et al. / Nonsense-mediated mRNA decay in Drosophila : at the intersection of the yeast and mammalian pathways. In: EMBO Journal. 2003 ; Vol. 22, No. 15. pp. 3960-3970.

Bibtex

@article{50da5ed5909040ca85a38bfcc2102068,
title = "Nonsense-mediated mRNA decay in Drosophila: at the intersection of the yeast and mammalian pathways",
abstract = "The nonsense-mediated mRNA decay (NMD) pathway promotes the rapid degradation of mRNAs containing premature stop codons (PTCs). In Caenorhabditis elegans, seven genes (smg1-7) playing an essential role in NMD have been identified. Only SMG2-4 (known as UPF1-3) have orthologs in Saccharomyces cerevisiae. Here we show that the Drosophila orthologs of UPF1-3, SMG1, SMG5 and SMG6 are required for the degradation of PTC-containing mRNAs, but that there is no SMG7 ortholog in this organism. In contrast, orthologs of SMG5-7 are encoded by the human genome and all three are required for NMD. In human cells, exon boundaries have been shown to play a critical role in defining PTCs. This role is mediated by components of the exon junction complex (EJC). Contrary to expectation, however, we show that the components of the EJC are dispensable for NMD in Drosophila cells. Consistently, PTC definition occurs independently of exon boundaries in Drosophila. Our findings reveal that despite conservation of the NMD machinery, different mechanisms have evolved to discriminate premature from natural stop codons in metazoa.",
keywords = "Amino Acid Sequence, Animals, Base Sequence, DNA Primers, Drosophila, Drosophila Proteins, Exons, Genes, Reporter, Hydrolysis, Molecular Sequence Data, Protein-Serine-Threonine Kinases, RNA, Messenger, Saccharomyces cerevisiae, Sequence Homology, Amino Acid",
author = "David Gatfield and Leonie Unterholzner and Ciccarelli, {Francesca D} and Peer Bork and Elisa Izaurralde",
year = "2003",
month = aug,
day = "1",
doi = "10.1093/emboj/cdg371",
language = "English",
volume = "22",
pages = "3960--3970",
journal = "EMBO Journal",
issn = "0261-4189",
publisher = "Nature Publishing Group",
number = "15",

}

RIS

TY - JOUR

T1 - Nonsense-mediated mRNA decay in Drosophila

T2 - at the intersection of the yeast and mammalian pathways

AU - Gatfield, David

AU - Unterholzner, Leonie

AU - Ciccarelli, Francesca D

AU - Bork, Peer

AU - Izaurralde, Elisa

PY - 2003/8/1

Y1 - 2003/8/1

N2 - The nonsense-mediated mRNA decay (NMD) pathway promotes the rapid degradation of mRNAs containing premature stop codons (PTCs). In Caenorhabditis elegans, seven genes (smg1-7) playing an essential role in NMD have been identified. Only SMG2-4 (known as UPF1-3) have orthologs in Saccharomyces cerevisiae. Here we show that the Drosophila orthologs of UPF1-3, SMG1, SMG5 and SMG6 are required for the degradation of PTC-containing mRNAs, but that there is no SMG7 ortholog in this organism. In contrast, orthologs of SMG5-7 are encoded by the human genome and all three are required for NMD. In human cells, exon boundaries have been shown to play a critical role in defining PTCs. This role is mediated by components of the exon junction complex (EJC). Contrary to expectation, however, we show that the components of the EJC are dispensable for NMD in Drosophila cells. Consistently, PTC definition occurs independently of exon boundaries in Drosophila. Our findings reveal that despite conservation of the NMD machinery, different mechanisms have evolved to discriminate premature from natural stop codons in metazoa.

AB - The nonsense-mediated mRNA decay (NMD) pathway promotes the rapid degradation of mRNAs containing premature stop codons (PTCs). In Caenorhabditis elegans, seven genes (smg1-7) playing an essential role in NMD have been identified. Only SMG2-4 (known as UPF1-3) have orthologs in Saccharomyces cerevisiae. Here we show that the Drosophila orthologs of UPF1-3, SMG1, SMG5 and SMG6 are required for the degradation of PTC-containing mRNAs, but that there is no SMG7 ortholog in this organism. In contrast, orthologs of SMG5-7 are encoded by the human genome and all three are required for NMD. In human cells, exon boundaries have been shown to play a critical role in defining PTCs. This role is mediated by components of the exon junction complex (EJC). Contrary to expectation, however, we show that the components of the EJC are dispensable for NMD in Drosophila cells. Consistently, PTC definition occurs independently of exon boundaries in Drosophila. Our findings reveal that despite conservation of the NMD machinery, different mechanisms have evolved to discriminate premature from natural stop codons in metazoa.

KW - Amino Acid Sequence

KW - Animals

KW - Base Sequence

KW - DNA Primers

KW - Drosophila

KW - Drosophila Proteins

KW - Exons

KW - Genes, Reporter

KW - Hydrolysis

KW - Molecular Sequence Data

KW - Protein-Serine-Threonine Kinases

KW - RNA, Messenger

KW - Saccharomyces cerevisiae

KW - Sequence Homology, Amino Acid

U2 - 10.1093/emboj/cdg371

DO - 10.1093/emboj/cdg371

M3 - Journal article

C2 - 12881430

VL - 22

SP - 3960

EP - 3970

JO - EMBO Journal

JF - EMBO Journal

SN - 0261-4189

IS - 15

ER -