Rights statement: This is the peer reviewed version of the following article: Dziadek, M, Kudlackova, R, Zima, A, et al. Novel multicomponent organic–inorganic WPI/gelatin/CaP hydrogel composites for bone tissue engineering. J Biomed Mater Res. 2019; 107A: 2479– 2491. https://doi.org/10.1002/jbm.a.36754 which has been published in final form at https://onlinelibrary.wiley.com/doi/full/10.1002/jbm.a.36754 This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
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Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Novel multicomponent organic-inorganic WPI/gelatin/CaP hydrogel composites for bone tissue engineering
AU - Dziadek, Michal
AU - Kudlackova, Radmila
AU - Zima, Aneta
AU - Slosarczyk, Anna
AU - Ziabka, Magdalena
AU - Jelen, Piotr
AU - Shkarina, Svetlana
AU - Cecilia, Angelica
AU - Zuber, Marcus
AU - Surmeneva, Maria
AU - Surmenev, Roman
AU - Bačáková, Lucie
AU - Cholewa-Kowalska, Katarzyna
AU - Douglas, Timothy
N1 - This is the peer reviewed version of the following article: Dziadek, M, Kudlackova, R, Zima, A, et al. Novel multicomponent organic–inorganic WPI/gelatin/CaP hydrogel composites for bone tissue engineering. J Biomed Mater Res. 2019; 107A: 2479– 2491. https://doi.org/10.1002/jbm.a.36754 which has been published in final form at https://onlinelibrary.wiley.com/doi/full/10.1002/jbm.a.36754 This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
PY - 2019/11/30
Y1 - 2019/11/30
N2 - The present work focuses on the development of novel multicomponent organic‐inorganic hydrogel composites for bone tissue engineering. For the first time, combination of the organic components commonly used in food industry, namely whey protein isolate (WPI) and gelatin from bovine skin, as well as inorganic material commonly used as a major component of hydraulic bone cements, namely α‐TCP in various concentrations (0‐70 wt.%) was proposed. The results showed that α‐TCP underwent incomplete transformation to calcium‐deficient hydroxyapatite (CDHA) during preparation process of the hydrogels. Microcomputer tomography showed inhomogeneous distribution of the calcium phosphate (CaP) phase in the resulting composites. Nevertheless, hydrogels containing 30‐70 wt.% α‐TCP showed significantly improved mechanical properties. The values of Young's modulus and the stresses corresponding to compression of a sample by 50% increased almost linearly with increasing concentration of ceramic phase. Incomplete transformation of α‐TCP to CDHA during preparation process of composites provides them high reactivity in simulated body fluid during 14‐day incubation. Preliminary in vitro studies revealed that the WPI/gelatin/CaP composite hydrogels support the adhesion, spreading, and proliferation of human osteoblast‐like MG‐63 cells. The WPI/gelatin/CaP composite hydrogels obtained in this work showed great potential for the use in bone tissue engineering and regenerative medicine applications.
AB - The present work focuses on the development of novel multicomponent organic‐inorganic hydrogel composites for bone tissue engineering. For the first time, combination of the organic components commonly used in food industry, namely whey protein isolate (WPI) and gelatin from bovine skin, as well as inorganic material commonly used as a major component of hydraulic bone cements, namely α‐TCP in various concentrations (0‐70 wt.%) was proposed. The results showed that α‐TCP underwent incomplete transformation to calcium‐deficient hydroxyapatite (CDHA) during preparation process of the hydrogels. Microcomputer tomography showed inhomogeneous distribution of the calcium phosphate (CaP) phase in the resulting composites. Nevertheless, hydrogels containing 30‐70 wt.% α‐TCP showed significantly improved mechanical properties. The values of Young's modulus and the stresses corresponding to compression of a sample by 50% increased almost linearly with increasing concentration of ceramic phase. Incomplete transformation of α‐TCP to CDHA during preparation process of composites provides them high reactivity in simulated body fluid during 14‐day incubation. Preliminary in vitro studies revealed that the WPI/gelatin/CaP composite hydrogels support the adhesion, spreading, and proliferation of human osteoblast‐like MG‐63 cells. The WPI/gelatin/CaP composite hydrogels obtained in this work showed great potential for the use in bone tissue engineering and regenerative medicine applications.
KW - whey protein isolate
KW - gelatin
KW - calcium phosphate
KW - hydrogel composites
U2 - 10.1002/jbm.a.36754
DO - 10.1002/jbm.a.36754
M3 - Journal article
VL - 107
SP - 2479
EP - 2491
JO - Journal of Biomedical Materials Research Part A
JF - Journal of Biomedical Materials Research Part A
SN - 1549-3296
IS - 11
ER -