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    Rights statement: This is the peer reviewed version of the following article: Dziadek, M, Kudlackova, R, Zima, A, et al. Novel multicomponent organic–inorganic WPI/gelatin/CaP hydrogel composites for bone tissue engineering. J Biomed Mater Res. 2019; 107A: 2479– 2491. https://doi.org/10.1002/jbm.a.36754 which has been published in final form at https://onlinelibrary.wiley.com/doi/full/10.1002/jbm.a.36754 This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.

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Novel multicomponent organic-inorganic WPI/gelatin/CaP hydrogel composites for bone tissue engineering

Research output: Contribution to journalJournal article

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Novel multicomponent organic-inorganic WPI/gelatin/CaP hydrogel composites for bone tissue engineering. / Dziadek, Michal; Kudlackova, Radmila; Zima, Aneta; Slosarczyk, Anna; Ziabka, Magdalena; Jelen, Piotr; Shkarina, Svetlana; Cecilia, Angelica; Zuber, Marcus; Surmeneva, Maria; Surmenev, Roman; Bačáková, Lucie; Cholewa-Kowalska, Katarzyna; Douglas, Timothy.

In: Journal of Biomedical Materials Research Part A, Vol. 107, No. 11, 30.11.2019, p. 2479-2491.

Research output: Contribution to journalJournal article

Harvard

Dziadek, M, Kudlackova, R, Zima, A, Slosarczyk, A, Ziabka, M, Jelen, P, Shkarina, S, Cecilia, A, Zuber, M, Surmeneva, M, Surmenev, R, Bačáková, L, Cholewa-Kowalska, K & Douglas, T 2019, 'Novel multicomponent organic-inorganic WPI/gelatin/CaP hydrogel composites for bone tissue engineering', Journal of Biomedical Materials Research Part A, vol. 107, no. 11, pp. 2479-2491. https://doi.org/10.1002/jbm.a.36754

APA

Dziadek, M., Kudlackova, R., Zima, A., Slosarczyk, A., Ziabka, M., Jelen, P., Shkarina, S., Cecilia, A., Zuber, M., Surmeneva, M., Surmenev, R., Bačáková, L., Cholewa-Kowalska, K., & Douglas, T. (2019). Novel multicomponent organic-inorganic WPI/gelatin/CaP hydrogel composites for bone tissue engineering. Journal of Biomedical Materials Research Part A, 107(11), 2479-2491. https://doi.org/10.1002/jbm.a.36754

Vancouver

Dziadek M, Kudlackova R, Zima A, Slosarczyk A, Ziabka M, Jelen P et al. Novel multicomponent organic-inorganic WPI/gelatin/CaP hydrogel composites for bone tissue engineering. Journal of Biomedical Materials Research Part A. 2019 Nov 30;107(11):2479-2491. https://doi.org/10.1002/jbm.a.36754

Author

Dziadek, Michal ; Kudlackova, Radmila ; Zima, Aneta ; Slosarczyk, Anna ; Ziabka, Magdalena ; Jelen, Piotr ; Shkarina, Svetlana ; Cecilia, Angelica ; Zuber, Marcus ; Surmeneva, Maria ; Surmenev, Roman ; Bačáková, Lucie ; Cholewa-Kowalska, Katarzyna ; Douglas, Timothy. / Novel multicomponent organic-inorganic WPI/gelatin/CaP hydrogel composites for bone tissue engineering. In: Journal of Biomedical Materials Research Part A. 2019 ; Vol. 107, No. 11. pp. 2479-2491.

Bibtex

@article{544bc6fe9f7a4722bf23171f9d8bf661,
title = "Novel multicomponent organic-inorganic WPI/gelatin/CaP hydrogel composites for bone tissue engineering",
abstract = "The present work focuses on the development of novel multicomponent organic‐inorganic hydrogel composites for bone tissue engineering. For the first time, combination of the organic components commonly used in food industry, namely whey protein isolate (WPI) and gelatin from bovine skin, as well as inorganic material commonly used as a major component of hydraulic bone cements, namely α‐TCP in various concentrations (0‐70 wt.%) was proposed. The results showed that α‐TCP underwent incomplete transformation to calcium‐deficient hydroxyapatite (CDHA) during preparation process of the hydrogels. Microcomputer tomography showed inhomogeneous distribution of the calcium phosphate (CaP) phase in the resulting composites. Nevertheless, hydrogels containing 30‐70 wt.% α‐TCP showed significantly improved mechanical properties. The values of Young's modulus and the stresses corresponding to compression of a sample by 50% increased almost linearly with increasing concentration of ceramic phase. Incomplete transformation of α‐TCP to CDHA during preparation process of composites provides them high reactivity in simulated body fluid during 14‐day incubation. Preliminary in vitro studies revealed that the WPI/gelatin/CaP composite hydrogels support the adhesion, spreading, and proliferation of human osteoblast‐like MG‐63 cells. The WPI/gelatin/CaP composite hydrogels obtained in this work showed great potential for the use in bone tissue engineering and regenerative medicine applications.",
keywords = "whey protein isolate, gelatin, calcium phosphate, hydrogel composites",
author = "Michal Dziadek and Radmila Kudlackova and Aneta Zima and Anna Slosarczyk and Magdalena Ziabka and Piotr Jelen and Svetlana Shkarina and Angelica Cecilia and Marcus Zuber and Maria Surmeneva and Roman Surmenev and Lucie Ba{\v c}{\'a}kov{\'a} and Katarzyna Cholewa-Kowalska and Timothy Douglas",
note = "This is the peer reviewed version of the following article: Dziadek, M, Kudlackova, R, Zima, A, et al. Novel multicomponent organic–inorganic WPI/gelatin/CaP hydrogel composites for bone tissue engineering. J Biomed Mater Res. 2019; 107A: 2479– 2491. https://doi.org/10.1002/jbm.a.36754 which has been published in final form at https://onlinelibrary.wiley.com/doi/full/10.1002/jbm.a.36754 This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving. ",
year = "2019",
month = nov
day = "30",
doi = "10.1002/jbm.a.36754",
language = "English",
volume = "107",
pages = "2479--2491",
journal = "Journal of Biomedical Materials Research Part A",
issn = "1549-3296",
publisher = "John Wiley and Sons Inc.",
number = "11",

}

RIS

TY - JOUR

T1 - Novel multicomponent organic-inorganic WPI/gelatin/CaP hydrogel composites for bone tissue engineering

AU - Dziadek, Michal

AU - Kudlackova, Radmila

AU - Zima, Aneta

AU - Slosarczyk, Anna

AU - Ziabka, Magdalena

AU - Jelen, Piotr

AU - Shkarina, Svetlana

AU - Cecilia, Angelica

AU - Zuber, Marcus

AU - Surmeneva, Maria

AU - Surmenev, Roman

AU - Bačáková, Lucie

AU - Cholewa-Kowalska, Katarzyna

AU - Douglas, Timothy

N1 - This is the peer reviewed version of the following article: Dziadek, M, Kudlackova, R, Zima, A, et al. Novel multicomponent organic–inorganic WPI/gelatin/CaP hydrogel composites for bone tissue engineering. J Biomed Mater Res. 2019; 107A: 2479– 2491. https://doi.org/10.1002/jbm.a.36754 which has been published in final form at https://onlinelibrary.wiley.com/doi/full/10.1002/jbm.a.36754 This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.

PY - 2019/11/30

Y1 - 2019/11/30

N2 - The present work focuses on the development of novel multicomponent organic‐inorganic hydrogel composites for bone tissue engineering. For the first time, combination of the organic components commonly used in food industry, namely whey protein isolate (WPI) and gelatin from bovine skin, as well as inorganic material commonly used as a major component of hydraulic bone cements, namely α‐TCP in various concentrations (0‐70 wt.%) was proposed. The results showed that α‐TCP underwent incomplete transformation to calcium‐deficient hydroxyapatite (CDHA) during preparation process of the hydrogels. Microcomputer tomography showed inhomogeneous distribution of the calcium phosphate (CaP) phase in the resulting composites. Nevertheless, hydrogels containing 30‐70 wt.% α‐TCP showed significantly improved mechanical properties. The values of Young's modulus and the stresses corresponding to compression of a sample by 50% increased almost linearly with increasing concentration of ceramic phase. Incomplete transformation of α‐TCP to CDHA during preparation process of composites provides them high reactivity in simulated body fluid during 14‐day incubation. Preliminary in vitro studies revealed that the WPI/gelatin/CaP composite hydrogels support the adhesion, spreading, and proliferation of human osteoblast‐like MG‐63 cells. The WPI/gelatin/CaP composite hydrogels obtained in this work showed great potential for the use in bone tissue engineering and regenerative medicine applications.

AB - The present work focuses on the development of novel multicomponent organic‐inorganic hydrogel composites for bone tissue engineering. For the first time, combination of the organic components commonly used in food industry, namely whey protein isolate (WPI) and gelatin from bovine skin, as well as inorganic material commonly used as a major component of hydraulic bone cements, namely α‐TCP in various concentrations (0‐70 wt.%) was proposed. The results showed that α‐TCP underwent incomplete transformation to calcium‐deficient hydroxyapatite (CDHA) during preparation process of the hydrogels. Microcomputer tomography showed inhomogeneous distribution of the calcium phosphate (CaP) phase in the resulting composites. Nevertheless, hydrogels containing 30‐70 wt.% α‐TCP showed significantly improved mechanical properties. The values of Young's modulus and the stresses corresponding to compression of a sample by 50% increased almost linearly with increasing concentration of ceramic phase. Incomplete transformation of α‐TCP to CDHA during preparation process of composites provides them high reactivity in simulated body fluid during 14‐day incubation. Preliminary in vitro studies revealed that the WPI/gelatin/CaP composite hydrogels support the adhesion, spreading, and proliferation of human osteoblast‐like MG‐63 cells. The WPI/gelatin/CaP composite hydrogels obtained in this work showed great potential for the use in bone tissue engineering and regenerative medicine applications.

KW - whey protein isolate

KW - gelatin

KW - calcium phosphate

KW - hydrogel composites

U2 - 10.1002/jbm.a.36754

DO - 10.1002/jbm.a.36754

M3 - Journal article

VL - 107

SP - 2479

EP - 2491

JO - Journal of Biomedical Materials Research Part A

JF - Journal of Biomedical Materials Research Part A

SN - 1549-3296

IS - 11

ER -