Home > Research > Publications & Outputs > Novel naturally derived whey protein isolate an...

Electronic data

  • Accepted manuscript and SI

    Accepted author manuscript, 26.4 MB, PDF document

    Available under license: CC BY-NC-ND: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License

Links

Text available via DOI:

View graph of relations

Novel naturally derived whey protein isolate and aragonite biocomposite hydrogels have potential for bone regeneration

Research output: Contribution to journalJournal article

Published

Standard

Novel naturally derived whey protein isolate and aragonite biocomposite hydrogels have potential for bone regeneration. / Gupta, Dhanak; Kocot, Magdalena; Tryba, Anna-Maria; Serafim, Andrada; Stancu, Izabela-Cristina; Jaegermann, Zbigniew; Pamula, Elzbieta; Reilly, G.C.; Douglas, Timothy.

In: Materials and Design, Vol. 188, 108408, 01.03.2020.

Research output: Contribution to journalJournal article

Harvard

APA

Vancouver

Author

Gupta, Dhanak ; Kocot, Magdalena ; Tryba, Anna-Maria ; Serafim, Andrada ; Stancu, Izabela-Cristina ; Jaegermann, Zbigniew ; Pamula, Elzbieta ; Reilly, G.C. ; Douglas, Timothy. / Novel naturally derived whey protein isolate and aragonite biocomposite hydrogels have potential for bone regeneration. In: Materials and Design. 2020 ; Vol. 188.

Bibtex

@article{f24b5eab4c354ac387528cba25f9727f,
title = "Novel naturally derived whey protein isolate and aragonite biocomposite hydrogels have potential for bone regeneration",
abstract = "This work explores novel biocomposite hydrogels fabricated using 40% (wt/vol) solution of whey protein isolate (WPI, from the food industry) mixed with increasing concentrations of synthetic aragonite rod-like powder of 0, 100, 200 and 300 mg/ml (named WPI0, WPI100, WPI200 and WPI300). FTIR results showed that aragonite was successfully incorporated into the WPI hydrogel network. SEM and micro-CT investigations revealed that aragonite was mainly concentrated near the edges of the composite samples, except in WPI300, which had homogeneous aragonite distribution. The pore diameters ranged from 18 to 778 μm while averaged pore size was the lowest for WPI0 at 30 μm and highest for WPI200 at 103 μm. The mean compression modulus was highest for WPI300 at 3.16 MPa. After 28 days in physiological conditions WPI300 had maximum mean swelling of 4.3% and there was the highest degradation rate for WPI200 and WPI300 and lowest for WPI100 and WPI0. The osteoblast-like MG63 cell metabolic and alkaline phosphatase activities in direct contact experiments with composites increased with increasing aragonite content over 3 weeks. Moreover, the degradation products of these composites were non-cytotoxic and led to mineral-like deposits in extracellular matrix. These WPI-aragonite biocomposite hydrogels are potent candidates for bone repair applications.",
keywords = "Whey protein isolate, Aragonite, Bone graft, Inexpensive, Degradation, Cytocompatible",
author = "Dhanak Gupta and Magdalena Kocot and Anna-Maria Tryba and Andrada Serafim and Izabela-Cristina Stancu and Zbigniew Jaegermann and Elzbieta Pamula and G.C. Reilly and Timothy Douglas",
year = "2020",
month = mar
day = "1",
doi = "10.1016/j.matdes.2019.108408",
language = "English",
volume = "188",
journal = "Materials and Design",
issn = "0261-3069",
publisher = "Elsevier Ltd",

}

RIS

TY - JOUR

T1 - Novel naturally derived whey protein isolate and aragonite biocomposite hydrogels have potential for bone regeneration

AU - Gupta, Dhanak

AU - Kocot, Magdalena

AU - Tryba, Anna-Maria

AU - Serafim, Andrada

AU - Stancu, Izabela-Cristina

AU - Jaegermann, Zbigniew

AU - Pamula, Elzbieta

AU - Reilly, G.C.

AU - Douglas, Timothy

PY - 2020/3/1

Y1 - 2020/3/1

N2 - This work explores novel biocomposite hydrogels fabricated using 40% (wt/vol) solution of whey protein isolate (WPI, from the food industry) mixed with increasing concentrations of synthetic aragonite rod-like powder of 0, 100, 200 and 300 mg/ml (named WPI0, WPI100, WPI200 and WPI300). FTIR results showed that aragonite was successfully incorporated into the WPI hydrogel network. SEM and micro-CT investigations revealed that aragonite was mainly concentrated near the edges of the composite samples, except in WPI300, which had homogeneous aragonite distribution. The pore diameters ranged from 18 to 778 μm while averaged pore size was the lowest for WPI0 at 30 μm and highest for WPI200 at 103 μm. The mean compression modulus was highest for WPI300 at 3.16 MPa. After 28 days in physiological conditions WPI300 had maximum mean swelling of 4.3% and there was the highest degradation rate for WPI200 and WPI300 and lowest for WPI100 and WPI0. The osteoblast-like MG63 cell metabolic and alkaline phosphatase activities in direct contact experiments with composites increased with increasing aragonite content over 3 weeks. Moreover, the degradation products of these composites were non-cytotoxic and led to mineral-like deposits in extracellular matrix. These WPI-aragonite biocomposite hydrogels are potent candidates for bone repair applications.

AB - This work explores novel biocomposite hydrogels fabricated using 40% (wt/vol) solution of whey protein isolate (WPI, from the food industry) mixed with increasing concentrations of synthetic aragonite rod-like powder of 0, 100, 200 and 300 mg/ml (named WPI0, WPI100, WPI200 and WPI300). FTIR results showed that aragonite was successfully incorporated into the WPI hydrogel network. SEM and micro-CT investigations revealed that aragonite was mainly concentrated near the edges of the composite samples, except in WPI300, which had homogeneous aragonite distribution. The pore diameters ranged from 18 to 778 μm while averaged pore size was the lowest for WPI0 at 30 μm and highest for WPI200 at 103 μm. The mean compression modulus was highest for WPI300 at 3.16 MPa. After 28 days in physiological conditions WPI300 had maximum mean swelling of 4.3% and there was the highest degradation rate for WPI200 and WPI300 and lowest for WPI100 and WPI0. The osteoblast-like MG63 cell metabolic and alkaline phosphatase activities in direct contact experiments with composites increased with increasing aragonite content over 3 weeks. Moreover, the degradation products of these composites were non-cytotoxic and led to mineral-like deposits in extracellular matrix. These WPI-aragonite biocomposite hydrogels are potent candidates for bone repair applications.

KW - Whey protein isolate

KW - Aragonite

KW - Bone graft

KW - Inexpensive

KW - Degradation

KW - Cytocompatible

U2 - 10.1016/j.matdes.2019.108408

DO - 10.1016/j.matdes.2019.108408

M3 - Journal article

VL - 188

JO - Materials and Design

JF - Materials and Design

SN - 0261-3069

M1 - 108408

ER -