Rights statement: This is the peer reviewed version of the following article: Douglas TEL, Schietse J, Zima A, Gorodzha S, Parakhonskiy BV, KhaleNkow D, Shkarin R, Ivanova A, Baumbach T, Weinhardt V, Stevens CV, Vanhoorne V, Vervaet C, Balcaen L, Vanhaecke F, Slośarczyk A, Surmeneva MA, Surmenev RA, Skirtach AG. 2018. Novel self-gelling injectable hydrogel/alpha-tricalcium phosphate composites for bone regeneration: Physiochemical and microcomputer tomographical characterization. J Biomed Mater Res Part A 2018:106A:822–828 which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.36277/abstract This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
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Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Novel self-gelling injectable hydrogel/alpha-tricalcium phosphate composites for bone regeneration
T2 - Physiochemical and microcomputer tomographical characterization
AU - Douglas, Timothy Edward Lim
AU - Schietse, Josefien
AU - Zima, Aneta
AU - Gorodzha, Svetlana
AU - Parakhonskiy, Bogdan
AU - Khalenkow, Dmitry
AU - Shkarin, Roman
AU - Ivanova, Anna
AU - Baumbach, Tilo
AU - Weinhardt, Venera
AU - Stevens, Christian
AU - Vanhoorne, Valerie
AU - Vervaet, Chris
AU - Balcaen, Lieve
AU - Vanhaecke, Frank
AU - Slosarczyk, Anna
AU - Surmeneva, Maria
AU - Surmenev, Roman
AU - Skirtach, Andre
N1 - This is the peer reviewed version of the following article: Douglas TEL, Schietse J, Zima A, Gorodzha S, Parakhonskiy BV, KhaleNkow D, Shkarin R, Ivanova A, Baumbach T, Weinhardt V, Stevens CV, Vanhoorne V, Vervaet C, Balcaen L, Vanhaecke F, Slośarczyk A, Surmeneva MA, Surmenev RA, Skirtach AG. 2018. Novel self-gelling injectable hydrogel/alpha-tricalcium phosphate composites for bone regeneration: Physiochemical and microcomputer tomographical characterization. J Biomed Mater Res Part A 2018:106A:822–828 which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/jbm.a.36277/abstract This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
PY - 2018/3
Y1 - 2018/3
N2 - Mineralized hydrogels are increasingly gaining attention as biomaterials for bone regeneration. The most common mineralization strategy has been addition of preformed inorganic particles during hydrogel formation. This maintains injectability. One common form of bone cement is formed by mixing particles of the highly reactive calcium phosphate alpha-tricalcium phosphate (α-TCP) with water to form hydroxyapatite (HA). The calcium ions released during this reaction can be exploited to crosslink anionic, calcium-binding polymers such as the polysaccharide gellan gum (GG) to induce hydrogel formation. In this study, three different amounts of α-TCP particles were added to GG polymer solution to generate novel, injectable hydrogel-inorganic composites. Distribution of the inorganic phase in the hydrogel was studied by high resolution microcomputer tomography (µCT). Gelation occurred within 30 min. α-TCP converted to HA. µCT revealed inhomogeneous distribution of the inorganic phase in the composites. These results demonstrate the potential of the composites as alternatives to traditional α-TCP bone cement and pave the way for incorporation of biologically active substances and in vitro and in vivo testing.
AB - Mineralized hydrogels are increasingly gaining attention as biomaterials for bone regeneration. The most common mineralization strategy has been addition of preformed inorganic particles during hydrogel formation. This maintains injectability. One common form of bone cement is formed by mixing particles of the highly reactive calcium phosphate alpha-tricalcium phosphate (α-TCP) with water to form hydroxyapatite (HA). The calcium ions released during this reaction can be exploited to crosslink anionic, calcium-binding polymers such as the polysaccharide gellan gum (GG) to induce hydrogel formation. In this study, three different amounts of α-TCP particles were added to GG polymer solution to generate novel, injectable hydrogel-inorganic composites. Distribution of the inorganic phase in the hydrogel was studied by high resolution microcomputer tomography (µCT). Gelation occurred within 30 min. α-TCP converted to HA. µCT revealed inhomogeneous distribution of the inorganic phase in the composites. These results demonstrate the potential of the composites as alternatives to traditional α-TCP bone cement and pave the way for incorporation of biologically active substances and in vitro and in vivo testing.
KW - hydrogel
KW - composite
KW - micro-CT
KW - gellan gum
KW - bone cement
U2 - 10.1002/jbm.a.36277
DO - 10.1002/jbm.a.36277
M3 - Journal article
VL - 106
SP - 822
EP - 828
JO - Journal of Biomedical Materials Research Part A
JF - Journal of Biomedical Materials Research Part A
SN - 1549-3296
IS - 3
ER -