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Nuclear and mitochondrial DNA of the primate malarial parasite Plasmodium knowlesi

Research output: Contribution to journalJournal article

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  • D H Williamson
  • R J Wilson
  • P A Bates
  • S McCready
  • F Perler
  • B U Qiang
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<mark>Journal publication date</mark>02/1985
<mark>Journal</mark>Molecular and Biochemical Parasitology
Issue number2
Volume14
Number of pages11
Pages (from-to)199-209
Publication statusPublished
Original languageEnglish

Abstract

Restriction analyses and DNA/DNA hybridisation of parasite DNA isolated from monkeys infected with the malarial parasite Plasmodium knowlesi has permitted unambiguous identification of the nuclear DNA of this species. Its (G+C) content, as determined by estimations of buoyant density as well as by direct analysis, is about 38%, essentially indistinguishable from that of its primate laboratory host, and grossly different from that of the major human malaria parasite, P. falciparum, which has a (G+C) content of approx. 19%. In addition, gradient fractionation of total P. knowlesi DNA revealed a minor DNA component (approx. 1% of the total) with a (G+C) content of about 19%. This DNA comprises covalently closed circular molecules which have a contour length about 11.6 microns, carry a small cruciform structure, and are thought to originate in the parasite's mitochondria.