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Nucleotide sequence analysis and epitope mapping of the merozoite surface protein 1 from Plasmodium chabaudi chabaudi AS.

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Nucleotide sequence analysis and epitope mapping of the merozoite surface protein 1 from Plasmodium chabaudi chabaudi AS. / McKean, Paul G.; O’Dea, Keiran P.; Brown, K. Neil.
In: Molecular and Biochemical Parasitology, Vol. 62, No. 2, 12.1993, p. 199-209.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

McKean, PG, O’Dea, KP & Brown, KN 1993, 'Nucleotide sequence analysis and epitope mapping of the merozoite surface protein 1 from Plasmodium chabaudi chabaudi AS.', Molecular and Biochemical Parasitology, vol. 62, no. 2, pp. 199-209. https://doi.org/10.1016/0166-6851(93)90109-B

APA

McKean, P. G., O’Dea, K. P., & Brown, K. N. (1993). Nucleotide sequence analysis and epitope mapping of the merozoite surface protein 1 from Plasmodium chabaudi chabaudi AS. Molecular and Biochemical Parasitology, 62(2), 199-209. https://doi.org/10.1016/0166-6851(93)90109-B

Vancouver

McKean PG, O’Dea KP, Brown KN. Nucleotide sequence analysis and epitope mapping of the merozoite surface protein 1 from Plasmodium chabaudi chabaudi AS. Molecular and Biochemical Parasitology. 1993 Dec;62(2):199-209. doi: 10.1016/0166-6851(93)90109-B

Author

McKean, Paul G. ; O’Dea, Keiran P. ; Brown, K. Neil. / Nucleotide sequence analysis and epitope mapping of the merozoite surface protein 1 from Plasmodium chabaudi chabaudi AS. In: Molecular and Biochemical Parasitology. 1993 ; Vol. 62, No. 2. pp. 199-209.

Bibtex

@article{d0fc03c491bf4e11a8f84c2f5f93ba41,
title = "Nucleotide sequence analysis and epitope mapping of the merozoite surface protein 1 from Plasmodium chabaudi chabaudi AS.",
abstract = "The complete nucleotide sequence of the gene encoding the merozoite surface protein 1 (MSP-1) from the rodent malaria parasite Plasmodium chabaudi chabaudi AS has been determined by direct sequencing of overlapping PCR derived fragments. Comparison of the P. c. chabaudi AS nucleotide sequence with the previously published P. c. chabaudi IP-PC1 sequence indicates that although the MSP-1 gene of these two P. c. chabaudi strains is highly conserved, with sequence identity often approaching 100%, interspersed throughout the molecule are 5 regions of divergence. This is at variance with published data which suggested that the P. c. chabaudi AS and P. c. chabaudi IP-PC1 MSP-1 sequences are largely identical. Epitope mapping studies with a panel of anti-P. c. chabaudi AS MSP-1 monoclonal antibodies demonstrate that whilst most of these mAbs recognise epitopes at the N-terminus of the MSP-1 molecule, two mAbs, including one capable of inhibiting challenge infections in mice in an in vivo passive transfer assay, recognise epitopes which map to the C-terminus.",
keywords = "Malaria, Plasmodium chabaudi chabaudi, Merozoite surface protein 1, Nucleotide sequence analysis, Epitope mapping",
author = "McKean, {Paul G.} and O{\textquoteright}Dea, {Keiran P.} and Brown, {K. Neil}",
year = "1993",
month = dec,
doi = "10.1016/0166-6851(93)90109-B",
language = "English",
volume = "62",
pages = "199--209",
journal = "Molecular and Biochemical Parasitology",
issn = "0166-6851",
publisher = "Elsevier",
number = "2",

}

RIS

TY - JOUR

T1 - Nucleotide sequence analysis and epitope mapping of the merozoite surface protein 1 from Plasmodium chabaudi chabaudi AS.

AU - McKean, Paul G.

AU - O’Dea, Keiran P.

AU - Brown, K. Neil

PY - 1993/12

Y1 - 1993/12

N2 - The complete nucleotide sequence of the gene encoding the merozoite surface protein 1 (MSP-1) from the rodent malaria parasite Plasmodium chabaudi chabaudi AS has been determined by direct sequencing of overlapping PCR derived fragments. Comparison of the P. c. chabaudi AS nucleotide sequence with the previously published P. c. chabaudi IP-PC1 sequence indicates that although the MSP-1 gene of these two P. c. chabaudi strains is highly conserved, with sequence identity often approaching 100%, interspersed throughout the molecule are 5 regions of divergence. This is at variance with published data which suggested that the P. c. chabaudi AS and P. c. chabaudi IP-PC1 MSP-1 sequences are largely identical. Epitope mapping studies with a panel of anti-P. c. chabaudi AS MSP-1 monoclonal antibodies demonstrate that whilst most of these mAbs recognise epitopes at the N-terminus of the MSP-1 molecule, two mAbs, including one capable of inhibiting challenge infections in mice in an in vivo passive transfer assay, recognise epitopes which map to the C-terminus.

AB - The complete nucleotide sequence of the gene encoding the merozoite surface protein 1 (MSP-1) from the rodent malaria parasite Plasmodium chabaudi chabaudi AS has been determined by direct sequencing of overlapping PCR derived fragments. Comparison of the P. c. chabaudi AS nucleotide sequence with the previously published P. c. chabaudi IP-PC1 sequence indicates that although the MSP-1 gene of these two P. c. chabaudi strains is highly conserved, with sequence identity often approaching 100%, interspersed throughout the molecule are 5 regions of divergence. This is at variance with published data which suggested that the P. c. chabaudi AS and P. c. chabaudi IP-PC1 MSP-1 sequences are largely identical. Epitope mapping studies with a panel of anti-P. c. chabaudi AS MSP-1 monoclonal antibodies demonstrate that whilst most of these mAbs recognise epitopes at the N-terminus of the MSP-1 molecule, two mAbs, including one capable of inhibiting challenge infections in mice in an in vivo passive transfer assay, recognise epitopes which map to the C-terminus.

KW - Malaria

KW - Plasmodium chabaudi chabaudi

KW - Merozoite surface protein 1

KW - Nucleotide sequence analysis

KW - Epitope mapping

U2 - 10.1016/0166-6851(93)90109-B

DO - 10.1016/0166-6851(93)90109-B

M3 - Journal article

VL - 62

SP - 199

EP - 209

JO - Molecular and Biochemical Parasitology

JF - Molecular and Biochemical Parasitology

SN - 0166-6851

IS - 2

ER -