Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Opportunities and limitations of molecular methods for quantifying microbial compliance parameters in EU bathing waters
AU - Oliver, David M.
AU - van Niekerk, Melanie
AU - Kay, David
AU - Heathwaite, A. Louise
AU - Porter, Jonathan
AU - Fleming, Lora E.
AU - Kinzelman, Julie L.
AU - Connolly, Elaine
AU - Cummins, Andy
AU - McPhail, Calum
AU - Rahman, Amanna
AU - Thairs, Ted
AU - de Roda Husman, Ana Maria
AU - Hanley, Nick D.
AU - Dunhill, Ian
AU - Globevnik, Lidija
AU - Harwood, Valerie J.
AU - Hodgson, Chris J.
AU - Lees, David N.
AU - Nichols, Gordon L.
AU - Nocker, Andreas
AU - Schets, Ciska
AU - Quilliam, Richard S.
N1 - Copyright © 2013 Elsevier Ltd. All rights reserved.
PY - 2014/3
Y1 - 2014/3
N2 - The debate over the suitability of molecular biological methods for the enumeration of regulatory microbial parameters (e.g. Faecal Indicator Organisms [FIOs]) in bathing waters versus the use of traditional culture-based methods is of current interest to regulators and the science community. Culture-based methods require a 24-48hour turn-around time from receipt at the laboratory to reporting, whilst quantitative molecular tools provide a more rapid assay (approximately 2-3h). Traditional culturing methods are therefore often viewed as slow and 'out-dated', although they still deliver an internationally 'accepted' evidence-base. In contrast, molecular tools have the potential for rapid analysis and their operational utility and associated limitations and uncertainties should be assessed in light of their use for regulatory monitoring. Here we report on the recommendations from a series of international workshops, chaired by a UK Working Group (WG) comprised of scientists, regulators, policy makers and other stakeholders, which explored and interrogated both molecular (principally quantitative polymerase chain reaction [qPCR]) and culture-based tools for FIO monitoring under the European Bathing Water Directive. Through detailed analysis of policy implications, regulatory barriers, stakeholder engagement, and the needs of the end-user, the WG identified a series of key concerns that require critical appraisal before a potential shift from culture-based approaches to the employment of molecular biological methods for bathing water regulation could be justified.
AB - The debate over the suitability of molecular biological methods for the enumeration of regulatory microbial parameters (e.g. Faecal Indicator Organisms [FIOs]) in bathing waters versus the use of traditional culture-based methods is of current interest to regulators and the science community. Culture-based methods require a 24-48hour turn-around time from receipt at the laboratory to reporting, whilst quantitative molecular tools provide a more rapid assay (approximately 2-3h). Traditional culturing methods are therefore often viewed as slow and 'out-dated', although they still deliver an internationally 'accepted' evidence-base. In contrast, molecular tools have the potential for rapid analysis and their operational utility and associated limitations and uncertainties should be assessed in light of their use for regulatory monitoring. Here we report on the recommendations from a series of international workshops, chaired by a UK Working Group (WG) comprised of scientists, regulators, policy makers and other stakeholders, which explored and interrogated both molecular (principally quantitative polymerase chain reaction [qPCR]) and culture-based tools for FIO monitoring under the European Bathing Water Directive. Through detailed analysis of policy implications, regulatory barriers, stakeholder engagement, and the needs of the end-user, the WG identified a series of key concerns that require critical appraisal before a potential shift from culture-based approaches to the employment of molecular biological methods for bathing water regulation could be justified.
KW - Compliance
KW - Environmental Monitoring
KW - Genetic Techniques
KW - Swimming
KW - Water Microbiology
KW - Water Quality
KW - Epidemiology
KW - EU Bathing Water Directive
KW - Faecal indicator organism
KW - Microbial pollution
KW - qPCR
KW - Recreational water
U2 - 10.1016/j.envint.2013.12.016
DO - 10.1016/j.envint.2013.12.016
M3 - Journal article
C2 - 24394589
VL - 64
SP - 124
EP - 128
JO - Environment International
JF - Environment International
SN - 0160-4120
ER -