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Opportunities and limitations of molecular methods for quantifying microbial compliance parameters in EU bathing waters

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Opportunities and limitations of molecular methods for quantifying microbial compliance parameters in EU bathing waters. / Oliver, David M.; van Niekerk, Melanie; Kay, David; Heathwaite, A. Louise; Porter, Jonathan; Fleming, Lora E.; Kinzelman, Julie L.; Connolly, Elaine; Cummins, Andy; McPhail, Calum; Rahman, Amanna; Thairs, Ted; de Roda Husman, Ana Maria; Hanley, Nick D.; Dunhill, Ian; Globevnik, Lidija; Harwood, Valerie J.; Hodgson, Chris J.; Lees, David N.; Nichols, Gordon L.; Nocker, Andreas; Schets, Ciska; Quilliam, Richard S.

In: Environment International, Vol. 64, 03.2014, p. 124-128.

Research output: Contribution to journalJournal article

Harvard

Oliver, DM, van Niekerk, M, Kay, D, Heathwaite, AL, Porter, J, Fleming, LE, Kinzelman, JL, Connolly, E, Cummins, A, McPhail, C, Rahman, A, Thairs, T, de Roda Husman, AM, Hanley, ND, Dunhill, I, Globevnik, L, Harwood, VJ, Hodgson, CJ, Lees, DN, Nichols, GL, Nocker, A, Schets, C & Quilliam, RS 2014, 'Opportunities and limitations of molecular methods for quantifying microbial compliance parameters in EU bathing waters', Environment International, vol. 64, pp. 124-128. https://doi.org/10.1016/j.envint.2013.12.016

APA

Oliver, D. M., van Niekerk, M., Kay, D., Heathwaite, A. L., Porter, J., Fleming, L. E., Kinzelman, J. L., Connolly, E., Cummins, A., McPhail, C., Rahman, A., Thairs, T., de Roda Husman, A. M., Hanley, N. D., Dunhill, I., Globevnik, L., Harwood, V. J., Hodgson, C. J., Lees, D. N., ... Quilliam, R. S. (2014). Opportunities and limitations of molecular methods for quantifying microbial compliance parameters in EU bathing waters. Environment International, 64, 124-128. https://doi.org/10.1016/j.envint.2013.12.016

Vancouver

Author

Oliver, David M. ; van Niekerk, Melanie ; Kay, David ; Heathwaite, A. Louise ; Porter, Jonathan ; Fleming, Lora E. ; Kinzelman, Julie L. ; Connolly, Elaine ; Cummins, Andy ; McPhail, Calum ; Rahman, Amanna ; Thairs, Ted ; de Roda Husman, Ana Maria ; Hanley, Nick D. ; Dunhill, Ian ; Globevnik, Lidija ; Harwood, Valerie J. ; Hodgson, Chris J. ; Lees, David N. ; Nichols, Gordon L. ; Nocker, Andreas ; Schets, Ciska ; Quilliam, Richard S. / Opportunities and limitations of molecular methods for quantifying microbial compliance parameters in EU bathing waters. In: Environment International. 2014 ; Vol. 64. pp. 124-128.

Bibtex

@article{d6bb01dfce474743813d0f17409913a3,
title = "Opportunities and limitations of molecular methods for quantifying microbial compliance parameters in EU bathing waters",
abstract = "The debate over the suitability of molecular biological methods for the enumeration of regulatory microbial parameters (e.g. Faecal Indicator Organisms [FIOs]) in bathing waters versus the use of traditional culture-based methods is of current interest to regulators and the science community. Culture-based methods require a 24-48hour turn-around time from receipt at the laboratory to reporting, whilst quantitative molecular tools provide a more rapid assay (approximately 2-3h). Traditional culturing methods are therefore often viewed as slow and 'out-dated', although they still deliver an internationally 'accepted' evidence-base. In contrast, molecular tools have the potential for rapid analysis and their operational utility and associated limitations and uncertainties should be assessed in light of their use for regulatory monitoring. Here we report on the recommendations from a series of international workshops, chaired by a UK Working Group (WG) comprised of scientists, regulators, policy makers and other stakeholders, which explored and interrogated both molecular (principally quantitative polymerase chain reaction [qPCR]) and culture-based tools for FIO monitoring under the European Bathing Water Directive. Through detailed analysis of policy implications, regulatory barriers, stakeholder engagement, and the needs of the end-user, the WG identified a series of key concerns that require critical appraisal before a potential shift from culture-based approaches to the employment of molecular biological methods for bathing water regulation could be justified.",
keywords = "Compliance, Environmental Monitoring, Genetic Techniques, Swimming, Water Microbiology, Water Quality, Epidemiology, EU Bathing Water Directive , Faecal indicator organism , Microbial pollution , qPCR , Recreational water",
author = "Oliver, {David M.} and {van Niekerk}, Melanie and David Kay and Heathwaite, {A. Louise} and Jonathan Porter and Fleming, {Lora E.} and Kinzelman, {Julie L.} and Elaine Connolly and Andy Cummins and Calum McPhail and Amanna Rahman and Ted Thairs and {de Roda Husman}, {Ana Maria} and Hanley, {Nick D.} and Ian Dunhill and Lidija Globevnik and Harwood, {Valerie J.} and Hodgson, {Chris J.} and Lees, {David N.} and Nichols, {Gordon L.} and Andreas Nocker and Ciska Schets and Quilliam, {Richard S.}",
note = "Copyright {\textcopyright} 2013 Elsevier Ltd. All rights reserved.",
year = "2014",
month = mar
doi = "10.1016/j.envint.2013.12.016",
language = "English",
volume = "64",
pages = "124--128",
journal = "Environment International",
issn = "0160-4120",
publisher = "Elsevier Ltd",

}

RIS

TY - JOUR

T1 - Opportunities and limitations of molecular methods for quantifying microbial compliance parameters in EU bathing waters

AU - Oliver, David M.

AU - van Niekerk, Melanie

AU - Kay, David

AU - Heathwaite, A. Louise

AU - Porter, Jonathan

AU - Fleming, Lora E.

AU - Kinzelman, Julie L.

AU - Connolly, Elaine

AU - Cummins, Andy

AU - McPhail, Calum

AU - Rahman, Amanna

AU - Thairs, Ted

AU - de Roda Husman, Ana Maria

AU - Hanley, Nick D.

AU - Dunhill, Ian

AU - Globevnik, Lidija

AU - Harwood, Valerie J.

AU - Hodgson, Chris J.

AU - Lees, David N.

AU - Nichols, Gordon L.

AU - Nocker, Andreas

AU - Schets, Ciska

AU - Quilliam, Richard S.

N1 - Copyright © 2013 Elsevier Ltd. All rights reserved.

PY - 2014/3

Y1 - 2014/3

N2 - The debate over the suitability of molecular biological methods for the enumeration of regulatory microbial parameters (e.g. Faecal Indicator Organisms [FIOs]) in bathing waters versus the use of traditional culture-based methods is of current interest to regulators and the science community. Culture-based methods require a 24-48hour turn-around time from receipt at the laboratory to reporting, whilst quantitative molecular tools provide a more rapid assay (approximately 2-3h). Traditional culturing methods are therefore often viewed as slow and 'out-dated', although they still deliver an internationally 'accepted' evidence-base. In contrast, molecular tools have the potential for rapid analysis and their operational utility and associated limitations and uncertainties should be assessed in light of their use for regulatory monitoring. Here we report on the recommendations from a series of international workshops, chaired by a UK Working Group (WG) comprised of scientists, regulators, policy makers and other stakeholders, which explored and interrogated both molecular (principally quantitative polymerase chain reaction [qPCR]) and culture-based tools for FIO monitoring under the European Bathing Water Directive. Through detailed analysis of policy implications, regulatory barriers, stakeholder engagement, and the needs of the end-user, the WG identified a series of key concerns that require critical appraisal before a potential shift from culture-based approaches to the employment of molecular biological methods for bathing water regulation could be justified.

AB - The debate over the suitability of molecular biological methods for the enumeration of regulatory microbial parameters (e.g. Faecal Indicator Organisms [FIOs]) in bathing waters versus the use of traditional culture-based methods is of current interest to regulators and the science community. Culture-based methods require a 24-48hour turn-around time from receipt at the laboratory to reporting, whilst quantitative molecular tools provide a more rapid assay (approximately 2-3h). Traditional culturing methods are therefore often viewed as slow and 'out-dated', although they still deliver an internationally 'accepted' evidence-base. In contrast, molecular tools have the potential for rapid analysis and their operational utility and associated limitations and uncertainties should be assessed in light of their use for regulatory monitoring. Here we report on the recommendations from a series of international workshops, chaired by a UK Working Group (WG) comprised of scientists, regulators, policy makers and other stakeholders, which explored and interrogated both molecular (principally quantitative polymerase chain reaction [qPCR]) and culture-based tools for FIO monitoring under the European Bathing Water Directive. Through detailed analysis of policy implications, regulatory barriers, stakeholder engagement, and the needs of the end-user, the WG identified a series of key concerns that require critical appraisal before a potential shift from culture-based approaches to the employment of molecular biological methods for bathing water regulation could be justified.

KW - Compliance

KW - Environmental Monitoring

KW - Genetic Techniques

KW - Swimming

KW - Water Microbiology

KW - Water Quality

KW - Epidemiology

KW - EU Bathing Water Directive

KW - Faecal indicator organism

KW - Microbial pollution

KW - qPCR

KW - Recreational water

U2 - 10.1016/j.envint.2013.12.016

DO - 10.1016/j.envint.2013.12.016

M3 - Journal article

C2 - 24394589

VL - 64

SP - 124

EP - 128

JO - Environment International

JF - Environment International

SN - 0160-4120

ER -