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Opsonization of polyethylene wear particles regulates macrophage and osteoblast responses in vitro

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Opsonization of polyethylene wear particles regulates macrophage and osteoblast responses in vitro. / Elfick, A. P. D.; Green, S. M.; McCaskie, A. W. et al.
In: Journal of Biomedical Materials Research Part B: Applied Biomaterials, Vol. 71B, No. 2, 15.11.2004, p. 244-251.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Elfick, APD, Green, SM, McCaskie, AW & Birch, MA 2004, 'Opsonization of polyethylene wear particles regulates macrophage and osteoblast responses in vitro', Journal of Biomedical Materials Research Part B: Applied Biomaterials, vol. 71B, no. 2, pp. 244-251. https://doi.org/10.1002/jbm.b.30095

APA

Elfick, A. P. D., Green, S. M., McCaskie, A. W., & Birch, M. A. (2004). Opsonization of polyethylene wear particles regulates macrophage and osteoblast responses in vitro. Journal of Biomedical Materials Research Part B: Applied Biomaterials, 71B(2), 244-251. https://doi.org/10.1002/jbm.b.30095

Vancouver

Elfick APD, Green SM, McCaskie AW, Birch MA. Opsonization of polyethylene wear particles regulates macrophage and osteoblast responses in vitro. Journal of Biomedical Materials Research Part B: Applied Biomaterials. 2004 Nov 15;71B(2):244-251. doi: 10.1002/jbm.b.30095

Author

Elfick, A. P. D. ; Green, S. M. ; McCaskie, A. W. et al. / Opsonization of polyethylene wear particles regulates macrophage and osteoblast responses in vitro. In: Journal of Biomedical Materials Research Part B: Applied Biomaterials. 2004 ; Vol. 71B, No. 2. pp. 244-251.

Bibtex

@article{59e91299cb4f4f07b6a96f9980d240f5,
title = "Opsonization of polyethylene wear particles regulates macrophage and osteoblast responses in vitro",
abstract = "The cellular reaction to wear debris may result in the failure of an artificial joint's fixation to the skeleton. The influence of debris opsinization on cell activity has received little attention. This study seeks to establish whether different proteinaceous culture environments may invoke variant cellular responses to debris challenge. Consideration of the zeta potential of a low density polyethylene particle group and an ex vitro ultrahigh molecular weight polyethylene particle group revealed that the nature of the protein adsorbants is related to the concentration of the proteins in solution. Furthermore, the composition of the adsorbed layer was shown to vary with the spectra of proteins in solution. In standard cell culture conditions zeta potential approached zero, indicating the high probability of particle agglomeration. Cell challenge studies with U937 macrophages showed that BSA and FCS protein adsorption mediated increased cell adhesion, while bovine IgG showed little change over control values. No changes in behavior of osteoblastic cells were observed in similar experiments. ",
author = "Elfick, {A. P. D.} and Green, {S. M.} and McCaskie, {A. W.} and Birch, {M. A.}",
year = "2004",
month = nov,
day = "15",
doi = "10.1002/jbm.b.30095",
language = "English",
volume = "71B",
pages = "244--251",
journal = "Journal of Biomedical Materials Research Part B: Applied Biomaterials",
issn = "1552-4973",
publisher = "John Wiley and Sons Inc.",
number = "2",

}

RIS

TY - JOUR

T1 - Opsonization of polyethylene wear particles regulates macrophage and osteoblast responses in vitro

AU - Elfick, A. P. D.

AU - Green, S. M.

AU - McCaskie, A. W.

AU - Birch, M. A.

PY - 2004/11/15

Y1 - 2004/11/15

N2 - The cellular reaction to wear debris may result in the failure of an artificial joint's fixation to the skeleton. The influence of debris opsinization on cell activity has received little attention. This study seeks to establish whether different proteinaceous culture environments may invoke variant cellular responses to debris challenge. Consideration of the zeta potential of a low density polyethylene particle group and an ex vitro ultrahigh molecular weight polyethylene particle group revealed that the nature of the protein adsorbants is related to the concentration of the proteins in solution. Furthermore, the composition of the adsorbed layer was shown to vary with the spectra of proteins in solution. In standard cell culture conditions zeta potential approached zero, indicating the high probability of particle agglomeration. Cell challenge studies with U937 macrophages showed that BSA and FCS protein adsorption mediated increased cell adhesion, while bovine IgG showed little change over control values. No changes in behavior of osteoblastic cells were observed in similar experiments.

AB - The cellular reaction to wear debris may result in the failure of an artificial joint's fixation to the skeleton. The influence of debris opsinization on cell activity has received little attention. This study seeks to establish whether different proteinaceous culture environments may invoke variant cellular responses to debris challenge. Consideration of the zeta potential of a low density polyethylene particle group and an ex vitro ultrahigh molecular weight polyethylene particle group revealed that the nature of the protein adsorbants is related to the concentration of the proteins in solution. Furthermore, the composition of the adsorbed layer was shown to vary with the spectra of proteins in solution. In standard cell culture conditions zeta potential approached zero, indicating the high probability of particle agglomeration. Cell challenge studies with U937 macrophages showed that BSA and FCS protein adsorption mediated increased cell adhesion, while bovine IgG showed little change over control values. No changes in behavior of osteoblastic cells were observed in similar experiments.

U2 - 10.1002/jbm.b.30095

DO - 10.1002/jbm.b.30095

M3 - Journal article

VL - 71B

SP - 244

EP - 251

JO - Journal of Biomedical Materials Research Part B: Applied Biomaterials

JF - Journal of Biomedical Materials Research Part B: Applied Biomaterials

SN - 1552-4973

IS - 2

ER -