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Optical control of NMDA-receptors with a diffusible photoswitch

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Optical control of NMDA-receptors with a diffusible photoswitch. / Laprell, Laura; Repak, Emilienne; Franckevicius, Vilius et al.
In: Nature Communications, Vol. 6, 8076, 27.08.2015.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Laprell, L, Repak, E, Franckevicius, V, Hartrampf, F, Terhag, J, Hollmann, M, Sumser, M, Rebola, N, Di Gregorio, D & Trauner, D 2015, 'Optical control of NMDA-receptors with a diffusible photoswitch', Nature Communications, vol. 6, 8076. https://doi.org/10.1038/ncomms9076

APA

Laprell, L., Repak, E., Franckevicius, V., Hartrampf, F., Terhag, J., Hollmann, M., Sumser, M., Rebola, N., Di Gregorio, D., & Trauner, D. (2015). Optical control of NMDA-receptors with a diffusible photoswitch. Nature Communications, 6, Article 8076. https://doi.org/10.1038/ncomms9076

Vancouver

Laprell L, Repak E, Franckevicius V, Hartrampf F, Terhag J, Hollmann M et al. Optical control of NMDA-receptors with a diffusible photoswitch. Nature Communications. 2015 Aug 27;6:8076. doi: 10.1038/ncomms9076

Author

Laprell, Laura ; Repak, Emilienne ; Franckevicius, Vilius et al. / Optical control of NMDA-receptors with a diffusible photoswitch. In: Nature Communications. 2015 ; Vol. 6.

Bibtex

@article{f8f1bfcc24c142f4b73eb32db76de007,
title = "Optical control of NMDA-receptors with a diffusible photoswitch",
abstract = "N-methyl-D-aspartate receptors (NMDARs) play a central role in synaptic plasticity, learning and memory, and are implicated in various neuronal disorders. We synthesized a diffusible photochromic glutamate analogue, azobenzene-triazole-glutamate (ATG), which is specific for NMDARs and functions as a photoswitchable agonist. ATG is inactive in its dark-adapted trans-isoform, but can be converted into its active cis-isoform using one-photon (near UV) or two-photon (740 nm) excitation. Irradiation with violet light photo-inactivates ATG within milliseconds, allowing agonist removal on the timescale of NMDAR deactivation. ATG is compatible with Ca2+ imaging and can be used to optically mimic synaptic coincidence detection protocols. Thus, ATG can be used like traditional caged glutamate compounds, but with the added advantages of NMDAR specificity, low antagonism of GABAR-mediated currents, and precise temporal control of agonist delivery.",
author = "Laura Laprell and Emilienne Repak and Vilius Franckevicius and Felix Hartrampf and Jan Terhag and M. Hollmann and Martin Sumser and Nelson Rebola and {Di Gregorio}, David and Dirk Trauner",
year = "2015",
month = aug,
day = "27",
doi = "10.1038/ncomms9076",
language = "English",
volume = "6",
journal = "Nature Communications",
issn = "2041-1723",
publisher = "Nature Publishing Group",

}

RIS

TY - JOUR

T1 - Optical control of NMDA-receptors with a diffusible photoswitch

AU - Laprell, Laura

AU - Repak, Emilienne

AU - Franckevicius, Vilius

AU - Hartrampf, Felix

AU - Terhag, Jan

AU - Hollmann, M.

AU - Sumser, Martin

AU - Rebola, Nelson

AU - Di Gregorio, David

AU - Trauner, Dirk

PY - 2015/8/27

Y1 - 2015/8/27

N2 - N-methyl-D-aspartate receptors (NMDARs) play a central role in synaptic plasticity, learning and memory, and are implicated in various neuronal disorders. We synthesized a diffusible photochromic glutamate analogue, azobenzene-triazole-glutamate (ATG), which is specific for NMDARs and functions as a photoswitchable agonist. ATG is inactive in its dark-adapted trans-isoform, but can be converted into its active cis-isoform using one-photon (near UV) or two-photon (740 nm) excitation. Irradiation with violet light photo-inactivates ATG within milliseconds, allowing agonist removal on the timescale of NMDAR deactivation. ATG is compatible with Ca2+ imaging and can be used to optically mimic synaptic coincidence detection protocols. Thus, ATG can be used like traditional caged glutamate compounds, but with the added advantages of NMDAR specificity, low antagonism of GABAR-mediated currents, and precise temporal control of agonist delivery.

AB - N-methyl-D-aspartate receptors (NMDARs) play a central role in synaptic plasticity, learning and memory, and are implicated in various neuronal disorders. We synthesized a diffusible photochromic glutamate analogue, azobenzene-triazole-glutamate (ATG), which is specific for NMDARs and functions as a photoswitchable agonist. ATG is inactive in its dark-adapted trans-isoform, but can be converted into its active cis-isoform using one-photon (near UV) or two-photon (740 nm) excitation. Irradiation with violet light photo-inactivates ATG within milliseconds, allowing agonist removal on the timescale of NMDAR deactivation. ATG is compatible with Ca2+ imaging and can be used to optically mimic synaptic coincidence detection protocols. Thus, ATG can be used like traditional caged glutamate compounds, but with the added advantages of NMDAR specificity, low antagonism of GABAR-mediated currents, and precise temporal control of agonist delivery.

U2 - 10.1038/ncomms9076

DO - 10.1038/ncomms9076

M3 - Journal article

VL - 6

JO - Nature Communications

JF - Nature Communications

SN - 2041-1723

M1 - 8076

ER -