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Overexpressing the ANR1 MADS-Box Gene in Transgenic Plants Provides New Insights into its Role in the Nitrate Regulation of Root Development

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Overexpressing the ANR1 MADS-Box Gene in Transgenic Plants Provides New Insights into its Role in the Nitrate Regulation of Root Development. / Gan, Yinbo; Bernreiter, Andreas; Filleur, Sophie et al.
In: Plant & cell physiology, Vol. 53, No. 6, 06.2012, p. 1003-1016.

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@article{2669a71342eb4783a9f9272f6ed9b3f4,
title = "Overexpressing the ANR1 MADS-Box Gene in Transgenic Plants Provides New Insights into its Role in the Nitrate Regulation of Root Development",
abstract = "The expression of the ANR1 MADS-box gene was manipulated in transgenic plants to investigate its role in the NO(3)(-)-dependent regulation of root development in Arabidopsis thaliana. Constitutive overexpression of ANR1 in roots, achieved using GAL4 enhancer trap lines, resulted in more rapid early seedling development, increased lengths and numbers of lateral roots and increased shoot fresh weight. Based on results obtained with five different enhancer trap lines, the overexpression of ANR1 in the lateral root tips appears to be more important for this phenotype than its level of expression in the developing lateral root primordia. Dexamethasone-mediated induction of ANR1 in lines expressing an ANR1-GR (glucocorticoid receptor) fusion protein stimulated lateral root growth but not primary root growth. Short-term (24 h) dexamethasone treatments led to prolonged stimulation of lateral root growth, whether the lateral roots were already mature or still unemerged at the time of treatment. In split-root experiments, localized application of dexamethasone to half of the root system of an ANR1-GR line elicited a localized increase in both the length and numbers of lateral roots, mimicking the effect of a localized NO(3)(-) treatment. In both types of transgenic line, the root phenotype was strongly dependent on the presence of NO(3)(-), indicating that there are additional components involved in ANR1 function that are NO(3)(-) regulated. The implications of these results for our understanding of ANR1's mode of action in the root response to localized NO(3)(-) are discussed.",
keywords = "Foraging , Nitrate signaling , Root architecture , Root behavior , Root development , Transgenic plants",
author = "Yinbo Gan and Andreas Bernreiter and Sophie Filleur and Beverley Abram and Forde, {Brian G}",
year = "2012",
month = jun,
doi = "10.1093/pcp/pcs050",
language = "English",
volume = "53",
pages = "1003--1016",
journal = "Plant & cell physiology",
issn = "1471-9053",
publisher = "Oxford University Press",
number = "6",

}

RIS

TY - JOUR

T1 - Overexpressing the ANR1 MADS-Box Gene in Transgenic Plants Provides New Insights into its Role in the Nitrate Regulation of Root Development

AU - Gan, Yinbo

AU - Bernreiter, Andreas

AU - Filleur, Sophie

AU - Abram, Beverley

AU - Forde, Brian G

PY - 2012/6

Y1 - 2012/6

N2 - The expression of the ANR1 MADS-box gene was manipulated in transgenic plants to investigate its role in the NO(3)(-)-dependent regulation of root development in Arabidopsis thaliana. Constitutive overexpression of ANR1 in roots, achieved using GAL4 enhancer trap lines, resulted in more rapid early seedling development, increased lengths and numbers of lateral roots and increased shoot fresh weight. Based on results obtained with five different enhancer trap lines, the overexpression of ANR1 in the lateral root tips appears to be more important for this phenotype than its level of expression in the developing lateral root primordia. Dexamethasone-mediated induction of ANR1 in lines expressing an ANR1-GR (glucocorticoid receptor) fusion protein stimulated lateral root growth but not primary root growth. Short-term (24 h) dexamethasone treatments led to prolonged stimulation of lateral root growth, whether the lateral roots were already mature or still unemerged at the time of treatment. In split-root experiments, localized application of dexamethasone to half of the root system of an ANR1-GR line elicited a localized increase in both the length and numbers of lateral roots, mimicking the effect of a localized NO(3)(-) treatment. In both types of transgenic line, the root phenotype was strongly dependent on the presence of NO(3)(-), indicating that there are additional components involved in ANR1 function that are NO(3)(-) regulated. The implications of these results for our understanding of ANR1's mode of action in the root response to localized NO(3)(-) are discussed.

AB - The expression of the ANR1 MADS-box gene was manipulated in transgenic plants to investigate its role in the NO(3)(-)-dependent regulation of root development in Arabidopsis thaliana. Constitutive overexpression of ANR1 in roots, achieved using GAL4 enhancer trap lines, resulted in more rapid early seedling development, increased lengths and numbers of lateral roots and increased shoot fresh weight. Based on results obtained with five different enhancer trap lines, the overexpression of ANR1 in the lateral root tips appears to be more important for this phenotype than its level of expression in the developing lateral root primordia. Dexamethasone-mediated induction of ANR1 in lines expressing an ANR1-GR (glucocorticoid receptor) fusion protein stimulated lateral root growth but not primary root growth. Short-term (24 h) dexamethasone treatments led to prolonged stimulation of lateral root growth, whether the lateral roots were already mature or still unemerged at the time of treatment. In split-root experiments, localized application of dexamethasone to half of the root system of an ANR1-GR line elicited a localized increase in both the length and numbers of lateral roots, mimicking the effect of a localized NO(3)(-) treatment. In both types of transgenic line, the root phenotype was strongly dependent on the presence of NO(3)(-), indicating that there are additional components involved in ANR1 function that are NO(3)(-) regulated. The implications of these results for our understanding of ANR1's mode of action in the root response to localized NO(3)(-) are discussed.

KW - Foraging

KW - Nitrate signaling

KW - Root architecture

KW - Root behavior

KW - Root development

KW - Transgenic plants

UR - http://www.scopus.com/inward/record.url?scp=84862179419&partnerID=8YFLogxK

U2 - 10.1093/pcp/pcs050

DO - 10.1093/pcp/pcs050

M3 - Journal article

C2 - 22523192

VL - 53

SP - 1003

EP - 1016

JO - Plant & cell physiology

JF - Plant & cell physiology

SN - 1471-9053

IS - 6

ER -