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Overexpression of ca1pase decreases Rubisco abundance and grain yield in wheat

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Overexpression of ca1pase decreases Rubisco abundance and grain yield in wheat. / Moreira Lobo, Ana; Orr, Douglas John; Oñate Gutierrez, Marta et al.
In: Plant Physiology, Vol. 181, No. 2, 01.10.2019, p. 471-479.

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Moreira Lobo A, Orr DJ, Oñate Gutierrez M, Andralojc PJ, Sparks C, Parry MAJ et al. Overexpression of ca1pase decreases Rubisco abundance and grain yield in wheat. Plant Physiology. 2019 Oct 1;181(2):471-479. Epub 2019 Jul 31. doi: 10.1104/pp.19.00693

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@article{c9fbdc2d04244bb6b41fcf54efcb6d29,
title = "Overexpression of ca1pase decreases Rubisco abundance and grain yield in wheat",
abstract = "Rubisco catalyzes the fixation of CO 2 into organic compounds that are used for plant growth and the production of agricultural products, and specific sugar-phosphate derivatives bind tightly to the active sites of Rubisco, locking the enzyme in a catalytically inactive conformation. 2-carboxy-d-arabinitol-1-phosphate phosphatase (CA1Pase) dephosphorylates such tight-binding inhibitors, contributing to the maintenance of Rubisco activity. Here, we investigated the hypothesis that overexpressing ca1pase would decrease the abundance of Rubisco inhibitors, thereby increasing the activity of Rubisco and enhancing photosynthetic performance and productivity in wheat ( Triticum aestivum). Plants of four independent wheat transgenic lines overexpressing ca1pase showed up to 30-fold increases in ca1pase expression compared to the wild type. Plants overexpressing ca1pase had lower numbers of Rubisco tight-binding inhibitors and higher Rubisco activation state than the wild type; however, there were 17% to 60% fewer Rubisco active sites in the four transgenic lines than in the wild type. The lower Rubisco content in plants overexpressing ca1pase resulted in lower initial and total carboxylating activities measured in flag leaves at the end of the vegetative stage and lower aboveground biomass and grain yield measured in fully mature plants. Hence, contrary to what would be expected, ca1pase overexpression decreased Rubisco content and compromised wheat grain yields. These results support a possible role for Rubisco inhibitors in protecting the enzyme and maintaining an adequate number of Rubisco active sites to support carboxylation rates in planta. ",
keywords = "CA1Pase, crop yield, gene expression, inhibition, regulation, Rubisco, tight-binding, wheat",
author = "{Moreira Lobo}, Ana and Orr, {Douglas John} and {O{\~n}ate Gutierrez}, Marta and Andralojc, {P. John} and Caroline Sparks and Parry, {Martin Afan John} and Elizabete Carmo-Silva",
note = "Copyright American Society of Plant Biologists",
year = "2019",
month = oct,
day = "1",
doi = "10.1104/pp.19.00693",
language = "English",
volume = "181",
pages = "471--479",
journal = "Plant Physiology",
issn = "0032-0889",
publisher = "American Society of Plant Biologists",
number = "2",

}

RIS

TY - JOUR

T1 - Overexpression of ca1pase decreases Rubisco abundance and grain yield in wheat

AU - Moreira Lobo, Ana

AU - Orr, Douglas John

AU - Oñate Gutierrez, Marta

AU - Andralojc, P. John

AU - Sparks, Caroline

AU - Parry, Martin Afan John

AU - Carmo-Silva, Elizabete

N1 - Copyright American Society of Plant Biologists

PY - 2019/10/1

Y1 - 2019/10/1

N2 - Rubisco catalyzes the fixation of CO 2 into organic compounds that are used for plant growth and the production of agricultural products, and specific sugar-phosphate derivatives bind tightly to the active sites of Rubisco, locking the enzyme in a catalytically inactive conformation. 2-carboxy-d-arabinitol-1-phosphate phosphatase (CA1Pase) dephosphorylates such tight-binding inhibitors, contributing to the maintenance of Rubisco activity. Here, we investigated the hypothesis that overexpressing ca1pase would decrease the abundance of Rubisco inhibitors, thereby increasing the activity of Rubisco and enhancing photosynthetic performance and productivity in wheat ( Triticum aestivum). Plants of four independent wheat transgenic lines overexpressing ca1pase showed up to 30-fold increases in ca1pase expression compared to the wild type. Plants overexpressing ca1pase had lower numbers of Rubisco tight-binding inhibitors and higher Rubisco activation state than the wild type; however, there were 17% to 60% fewer Rubisco active sites in the four transgenic lines than in the wild type. The lower Rubisco content in plants overexpressing ca1pase resulted in lower initial and total carboxylating activities measured in flag leaves at the end of the vegetative stage and lower aboveground biomass and grain yield measured in fully mature plants. Hence, contrary to what would be expected, ca1pase overexpression decreased Rubisco content and compromised wheat grain yields. These results support a possible role for Rubisco inhibitors in protecting the enzyme and maintaining an adequate number of Rubisco active sites to support carboxylation rates in planta.

AB - Rubisco catalyzes the fixation of CO 2 into organic compounds that are used for plant growth and the production of agricultural products, and specific sugar-phosphate derivatives bind tightly to the active sites of Rubisco, locking the enzyme in a catalytically inactive conformation. 2-carboxy-d-arabinitol-1-phosphate phosphatase (CA1Pase) dephosphorylates such tight-binding inhibitors, contributing to the maintenance of Rubisco activity. Here, we investigated the hypothesis that overexpressing ca1pase would decrease the abundance of Rubisco inhibitors, thereby increasing the activity of Rubisco and enhancing photosynthetic performance and productivity in wheat ( Triticum aestivum). Plants of four independent wheat transgenic lines overexpressing ca1pase showed up to 30-fold increases in ca1pase expression compared to the wild type. Plants overexpressing ca1pase had lower numbers of Rubisco tight-binding inhibitors and higher Rubisco activation state than the wild type; however, there were 17% to 60% fewer Rubisco active sites in the four transgenic lines than in the wild type. The lower Rubisco content in plants overexpressing ca1pase resulted in lower initial and total carboxylating activities measured in flag leaves at the end of the vegetative stage and lower aboveground biomass and grain yield measured in fully mature plants. Hence, contrary to what would be expected, ca1pase overexpression decreased Rubisco content and compromised wheat grain yields. These results support a possible role for Rubisco inhibitors in protecting the enzyme and maintaining an adequate number of Rubisco active sites to support carboxylation rates in planta.

KW - CA1Pase

KW - crop yield

KW - gene expression

KW - inhibition

KW - regulation

KW - Rubisco

KW - tight-binding

KW - wheat

U2 - 10.1104/pp.19.00693

DO - 10.1104/pp.19.00693

M3 - Journal article

C2 - 31366720

VL - 181

SP - 471

EP - 479

JO - Plant Physiology

JF - Plant Physiology

SN - 0032-0889

IS - 2

ER -