Rights statement: This is the author’s version of a work that was accepted for publication in Materials Letters. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Materials Letters, 227, 2018 DOI: 10.1016/j.matlet.2018.05.060
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Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Pectin coatings on titanium alloy scaffolds produced by additive manufacturing
T2 - Promotion of human bone marrow stromal cell proliferation
AU - Douglas, Timothy Edward Lim
AU - Hempel, Ute
AU - Zydek, Jagoda
AU - Vladescu, Alina
AU - Pietryga, Krzysztof
AU - Kaeswurm, Julia
AU - Buchweitz, Maria
AU - Surmenev, Roman
AU - Surmeneva, Maria
AU - Cotrut, Cosmin
AU - Koptyug, Andrey
AU - Pamula, Elzbieta
N1 - This is the author’s version of a work that was accepted for publication in Materials Letters. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Materials Letters, 227, 2018 DOI: 10.1016/j.matlet.2018.05.060
PY - 2018/9/15
Y1 - 2018/9/15
N2 - Ti6Al4V is a popular biomaterial for load-bearing implants for bone contact, which can be fabricated by additive manufacturing technologies. Their long-term success depends on their stable anchoring in surrounding bone, which in turn depends on formation of new bone tissue on the implant surface, for which adhesion and proliferation of bone-forming cells is a pre-requisite. Hence, surface coatings which promote cell adhesion and proliferation are desirable.Here, Ti6Al4V discs prepared by additive manufacturing (EBM) were coated with layers of pectins, calcium-binding polysaccharides derived from citrus (C) and apple (A), which also contained alkaline phosphatase (ALP), the enzyme responsible for mineralization of bone tissue.Adhesion and proliferation of human bone marrow stromal cells (hBMSC) were assessed. Proliferation after 7 days was increased by A-ALP coatings and, in particular, by C-ALP coatings. Cell morphology was similar on coated and uncoated samples. In conclusion, ALP-loaded pectin coatings promote hBMSC adhesion and proliferation.
AB - Ti6Al4V is a popular biomaterial for load-bearing implants for bone contact, which can be fabricated by additive manufacturing technologies. Their long-term success depends on their stable anchoring in surrounding bone, which in turn depends on formation of new bone tissue on the implant surface, for which adhesion and proliferation of bone-forming cells is a pre-requisite. Hence, surface coatings which promote cell adhesion and proliferation are desirable.Here, Ti6Al4V discs prepared by additive manufacturing (EBM) were coated with layers of pectins, calcium-binding polysaccharides derived from citrus (C) and apple (A), which also contained alkaline phosphatase (ALP), the enzyme responsible for mineralization of bone tissue.Adhesion and proliferation of human bone marrow stromal cells (hBMSC) were assessed. Proliferation after 7 days was increased by A-ALP coatings and, in particular, by C-ALP coatings. Cell morphology was similar on coated and uncoated samples. In conclusion, ALP-loaded pectin coatings promote hBMSC adhesion and proliferation.
KW - Biomaterials
KW - Thin films
KW - Biomimetic
U2 - 10.1016/j.matlet.2018.05.060
DO - 10.1016/j.matlet.2018.05.060
M3 - Journal article
VL - 227
SP - 225
EP - 228
JO - Materials Letters
JF - Materials Letters
SN - 0167-577X
ER -