Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Performance of Seven Commercial Phytases in an in Vitro Simulation of Poultry Digestive Tract
AU - Menezes-Blackburn, Daniel
AU - Gabler, Stefanie
AU - Greiner, Ralf
PY - 2015/7/15
Y1 - 2015/7/15
N2 - The aim of,this study was to compare the biochemical, properties of seven commercially, available phytase products as well as their catalytic performance in an in vitro simulation of the digestive tract of poultry. Their enzymatic properties relevant with respect to phytate dephosphorylation in the digestive tract of birds were determined under identical assay conditions. All phytase-products included in the study showed an acid pH optimum of activity and were capable of releasing the organically bound phosphate from phytate during the in vitro simulation. However, their overall biochemical properties and relative catalytic performances were remarkably different The in vitro degradation system was considered as a simple and useful tool to evaluate the suitability of a phytase to be used as a feed supplement. Although relevant factors such as dietary P levels, intestinal phytase, and P absorption ate not implemented in the system, this approach might help to reduce the number of feeding trials necessary in the, search for a better Suited phytase for animal feeding application.
AB - The aim of,this study was to compare the biochemical, properties of seven commercially, available phytase products as well as their catalytic performance in an in vitro simulation of the digestive tract of poultry. Their enzymatic properties relevant with respect to phytate dephosphorylation in the digestive tract of birds were determined under identical assay conditions. All phytase-products included in the study showed an acid pH optimum of activity and were capable of releasing the organically bound phosphate from phytate during the in vitro simulation. However, their overall biochemical properties and relative catalytic performances were remarkably different The in vitro degradation system was considered as a simple and useful tool to evaluate the suitability of a phytase to be used as a feed supplement. Although relevant factors such as dietary P levels, intestinal phytase, and P absorption ate not implemented in the system, this approach might help to reduce the number of feeding trials necessary in the, search for a better Suited phytase for animal feeding application.
KW - in vitro degradation system
KW - phytase
KW - phytate dephosphorylation
KW - PHOSPHORUS AVAILABILITY
KW - MICROBIAL ORIGINS
KW - ESCHERICHIA-COLI
KW - PENIOPHORA-LYCII
KW - PURIFICATION
KW - PHOSPHATE
KW - APPA
KW - PHYA
U2 - 10.1021/acs.jafc.5b01996
DO - 10.1021/acs.jafc.5b01996
M3 - Journal article
VL - 63
SP - 6142
EP - 6149
JO - Journal of Agricultural and Food Chemistry
JF - Journal of Agricultural and Food Chemistry
SN - 0021-8561
IS - 27
ER -