Rights statement: This is the peer reviewed version of the following article: Valluru, R., Reynolds, M. P., Davies, W. J. and Sukumaran, S. (2017), Phenotypic and genome-wide association analysis of spike ethylene in diverse wheat genotypes under heat stress. New Phytol, 214: 271–283. doi:10.1111/nph.14367 which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1111/nph.14367/abstract This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Phenotypic and genome-wide association analysis of spike ethylene in diverse wheat genotypes under heat stress
AU - Valluru, Ravi
AU - Reynolds, Matthew P.
AU - Davies, William John
AU - Sukumaran, Sivakumar
PY - 2017/4
Y1 - 2017/4
N2 - The gaseous phytohormone ethylene plays an important role in spike development in wheat (Triticum aestivum). However, the genotypic variation and the genomic regions governing spike ethylene (SET) production in wheat under long-term heat stress remain unexplored. We investigated genotypic variation in the production of SET and its relationship with spike dry weight (SDW) in 130 diverse wheat elite lines and landraces under heat-stressed field conditions.We employed an Illumina iSelect 90K single nucleotide polymorphism (SNP) genotyping array to identify the genetic loci for SET and SDW through a genome-wide association study (GWAS) in a subset of the Wheat Association Mapping Initiative (WAMI) panel. The SET and SDW exhibited appreciable genotypic variation among wheat genotypes at the anthesis stage. There was a strong negative correlation between SET and SDW. The GWAS uncovered five and 32 significant SNPs for SET, and 22 and 142 significant SNPs for SDW, in glasshouse and field conditions, respectively. Some of these SNPs closely localized tothe SNPs for plant height, suggesting close associations between plant height and spike related traits. The phenotypic and genetic elucidation of SET and its relationship with SDW supports future efforts toward gene discovery and breeding wheat cultivars with reduced ethylene effects on yield under heat stress.
AB - The gaseous phytohormone ethylene plays an important role in spike development in wheat (Triticum aestivum). However, the genotypic variation and the genomic regions governing spike ethylene (SET) production in wheat under long-term heat stress remain unexplored. We investigated genotypic variation in the production of SET and its relationship with spike dry weight (SDW) in 130 diverse wheat elite lines and landraces under heat-stressed field conditions.We employed an Illumina iSelect 90K single nucleotide polymorphism (SNP) genotyping array to identify the genetic loci for SET and SDW through a genome-wide association study (GWAS) in a subset of the Wheat Association Mapping Initiative (WAMI) panel. The SET and SDW exhibited appreciable genotypic variation among wheat genotypes at the anthesis stage. There was a strong negative correlation between SET and SDW. The GWAS uncovered five and 32 significant SNPs for SET, and 22 and 142 significant SNPs for SDW, in glasshouse and field conditions, respectively. Some of these SNPs closely localized tothe SNPs for plant height, suggesting close associations between plant height and spike related traits. The phenotypic and genetic elucidation of SET and its relationship with SDW supports future efforts toward gene discovery and breeding wheat cultivars with reduced ethylene effects on yield under heat stress.
KW - ethylene
KW - genome-wide association (GWA)
KW - heat stress
KW - spike dry weight
KW - spike ethylene
KW - wheat
U2 - 10.1111/nph.14367
DO - 10.1111/nph.14367
M3 - Journal article
VL - 214
SP - 271
EP - 283
JO - New Phytologist
JF - New Phytologist
SN - 0028-646X
IS - 1
ER -