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Phospholiphase C is required for the control of stomatal aperture by ABA.

Research output: Contribution to journalJournal article

Published

  • L. Hunt
  • L. N. Mills
  • C. Pical
  • C. P. Leckie
  • F. L. Aitken
  • J. Kopka
  • B. Mueller-Roeber
  • Martin McAinsh
  • A. M. Hetherington
  • J. E. Gray
Journal publication date04/2003
JournalPlant Journal
Journal number1
Volume34
Number of pages9
Pages47-55
Original languageEnglish

Abstract

The calcium-releasing second messenger inositol 1,4,5-trisphosphate is involved in the regulation of stomatal aperture by ABA. In other signalling pathways, inositol 1,4,5-trisphosphate is generated by the action of phospholipase C. We have studied the importance of phospholipase C in guard cell ABA-signalling pathways. Immunolocalisation of a calcium-activated phospholipase C confirmed the presence of phospholipase C in tobacco guard cells. Transgenic tobacco plants with considerably reduced levels of phospholipase C in their guard cells were only partially able to regulate their stomatal apertures in response to ABA. These results suggest that phospholipase C is involved in the amplification of the calcium signal responsible for reductions in stomatal aperture in response to ABA. As full ABA-induced inhibition of stomatal opening was not observed, our results support a role for the action of other calcium-releasing second messengers in the guard cell ABA-signalling pathway. It is not known whether these different calcium-releasing second messengers act in the same or parallel ABA-signalling pathways.