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    Rights statement: This is the peer reviewed version of the following article: Sowoidnich, K., Churchwell, J. H., Buckley, K., Goodship, A. E., Parker, A. W., and Matousek, P. (2016) Photon migration of Raman signal in bone as measured with spatially offset Raman spectroscopy. J. Raman Spectrosc., 47: 240–247. doi: 10.1002/jrs.4781 which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/jrs.4781/abstract This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.

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Photon migration of Raman signal in bone as measured with spatially offset Raman spectroscopy

Research output: Contribution to journalJournal article

Published
  • Kay Sowoidnich
  • John Churchwell
  • Jemma Gillian Kerns
  • Kevin Buckley
  • Allen Goodship
  • Anthony W. Parker
  • Pavel Matousek
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<mark>Journal publication date</mark>02/2016
<mark>Journal</mark>Journal of Raman Spectroscopy
Issue number2
Volume47
Number of pages8
Pages (from-to)240-247
Publication statusPublished
Early online date11/09/15
Original languageEnglish

Abstract

Spatially offset Raman spectroscopy (SORS) is currently being developed as an
in vivo tool for bone disease detection, but to date, information about the interrogated volume as influenced by the light propagation and scattering characteristics of the bone matrix is still limited. This paper seeks to develop our general understanding of the sampling depths of SORS in bone specimens as a function of the applied spatial offset. Equine metacarpal bone was selected as a suitable specimen of compact cortical bone large enough to allow several thin slices (600 μm) to be cut from the dorsal surface. Photon migration at 830-nm excitation was studied with five bone slices and a 380 μm-thin polytetrafluoroethylene (PTFE) slice placed consecutively between the layers. To optimize Raman signal recovery of the PTFE with increasing depth within the bone stack required a corresponding increase in spatial offset. For example, to sample effectively at 2.2-mm depth within the bone required an optimal SORS offset of 7mm. However, with a 7-mm offset, the maximum accessible penetration depth from which the PTFE signal could be still recovered was 3.7mm. These results provide essential basic information for developing SORS technology for medical diagnostics in general and optimizing sampling through bone tissue, permitting a better understanding of the relationship between the offset and depth of bone assessed, in particular. Potential applications include the detection of chemically specific markers for changes in bone matrix chemistry localized within the tissue and not present in healthy bone.

Bibliographic note

This is the peer reviewed version of the following article: Sowoidnich, K., Churchwell, J. H., Buckley, K., Goodship, A. E., Parker, A. W., and Matousek, P. (2016) Photon migration of Raman signal in bone as measured with spatially offset Raman spectroscopy. J. Raman Spectrosc., 47: 240–247. doi: 10.1002/jrs.4781 which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/jrs.4781/abstract This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.