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    Rights statement: This is the peer reviewed version of the following article: Sowoidnich, K., Churchwell, J. H., Buckley, K., Goodship, A. E., Parker, A. W., and Matousek, P. (2016) Photon migration of Raman signal in bone as measured with spatially offset Raman spectroscopy. J. Raman Spectrosc., 47: 240–247. doi: 10.1002/jrs.4781 which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/jrs.4781/abstract This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.

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Photon migration of Raman signal in bone as measured with spatially offset Raman spectroscopy

Research output: Contribution to journalJournal article

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Photon migration of Raman signal in bone as measured with spatially offset Raman spectroscopy. / Sowoidnich, Kay ; Churchwell, John; Kerns, Jemma Gillian; Buckley, Kevin; Goodship, Allen; Parker, Anthony W.; Matousek, Pavel.

In: Journal of Raman Spectroscopy, Vol. 47, No. 2, 02.2016, p. 240-247.

Research output: Contribution to journalJournal article

Harvard

Sowoidnich, K, Churchwell, J, Kerns, JG, Buckley, K, Goodship, A, Parker, AW & Matousek, P 2016, 'Photon migration of Raman signal in bone as measured with spatially offset Raman spectroscopy', Journal of Raman Spectroscopy, vol. 47, no. 2, pp. 240-247. https://doi.org/10.1002/jrs.4781

APA

Sowoidnich, K., Churchwell, J., Kerns, J. G., Buckley, K., Goodship, A., Parker, A. W., & Matousek, P. (2016). Photon migration of Raman signal in bone as measured with spatially offset Raman spectroscopy. Journal of Raman Spectroscopy, 47(2), 240-247. https://doi.org/10.1002/jrs.4781

Vancouver

Sowoidnich K, Churchwell J, Kerns JG, Buckley K, Goodship A, Parker AW et al. Photon migration of Raman signal in bone as measured with spatially offset Raman spectroscopy. Journal of Raman Spectroscopy. 2016 Feb;47(2):240-247. https://doi.org/10.1002/jrs.4781

Author

Sowoidnich, Kay ; Churchwell, John ; Kerns, Jemma Gillian ; Buckley, Kevin ; Goodship, Allen ; Parker, Anthony W. ; Matousek, Pavel. / Photon migration of Raman signal in bone as measured with spatially offset Raman spectroscopy. In: Journal of Raman Spectroscopy. 2016 ; Vol. 47, No. 2. pp. 240-247.

Bibtex

@article{039abcb78e234ea8b9ffe948acdb9482,
title = "Photon migration of Raman signal in bone as measured with spatially offset Raman spectroscopy",
abstract = "Spatially offset Raman spectroscopy (SORS) is currently being developed as anin vivo tool for bone disease detection, but to date, information about the interrogated volume as influenced by the light propagation and scattering characteristics of the bone matrix is still limited. This paper seeks to develop our general understanding of the sampling depths of SORS in bone specimens as a function of the applied spatial offset. Equine metacarpal bone was selected as a suitable specimen of compact cortical bone large enough to allow several thin slices (600 μm) to be cut from the dorsal surface. Photon migration at 830-nm excitation was studied with five bone slices and a 380 μm-thin polytetrafluoroethylene (PTFE) slice placed consecutively between the layers. To optimize Raman signal recovery of the PTFE with increasing depth within the bone stack required a corresponding increase in spatial offset. For example, to sample effectively at 2.2-mm depth within the bone required an optimal SORS offset of 7mm. However, with a 7-mm offset, the maximum accessible penetration depth from which the PTFE signal could be still recovered was 3.7mm. These results provide essential basic information for developing SORS technology for medical diagnostics in general and optimizing sampling through bone tissue, permitting a better understanding of the relationship between the offset and depth of bone assessed, in particular. Potential applications include the detection of chemically specific markers for changes in bone matrix chemistry localized within the tissue and not present in healthy bone. ",
keywords = "spatially offset Raman spectroscopy (SORS), bone, photon migration, diffuse scattering",
author = "Kay Sowoidnich and John Churchwell and Kerns, {Jemma Gillian} and Kevin Buckley and Allen Goodship and Parker, {Anthony W.} and Pavel Matousek",
note = "This is the peer reviewed version of the following article: Sowoidnich, K., Churchwell, J. H., Buckley, K., Goodship, A. E., Parker, A. W., and Matousek, P. (2016) Photon migration of Raman signal in bone as measured with spatially offset Raman spectroscopy. J. Raman Spectrosc., 47: 240–247. doi: 10.1002/jrs.4781 which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/jrs.4781/abstract This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.",
year = "2016",
month = feb
doi = "10.1002/jrs.4781",
language = "English",
volume = "47",
pages = "240--247",
journal = "Journal of Raman Spectroscopy",
issn = "0377-0486",
publisher = "John Wiley and Sons Ltd",
number = "2",

}

RIS

TY - JOUR

T1 - Photon migration of Raman signal in bone as measured with spatially offset Raman spectroscopy

AU - Sowoidnich, Kay

AU - Churchwell, John

AU - Kerns, Jemma Gillian

AU - Buckley, Kevin

AU - Goodship, Allen

AU - Parker, Anthony W.

AU - Matousek, Pavel

N1 - This is the peer reviewed version of the following article: Sowoidnich, K., Churchwell, J. H., Buckley, K., Goodship, A. E., Parker, A. W., and Matousek, P. (2016) Photon migration of Raman signal in bone as measured with spatially offset Raman spectroscopy. J. Raman Spectrosc., 47: 240–247. doi: 10.1002/jrs.4781 which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/jrs.4781/abstract This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.

PY - 2016/2

Y1 - 2016/2

N2 - Spatially offset Raman spectroscopy (SORS) is currently being developed as anin vivo tool for bone disease detection, but to date, information about the interrogated volume as influenced by the light propagation and scattering characteristics of the bone matrix is still limited. This paper seeks to develop our general understanding of the sampling depths of SORS in bone specimens as a function of the applied spatial offset. Equine metacarpal bone was selected as a suitable specimen of compact cortical bone large enough to allow several thin slices (600 μm) to be cut from the dorsal surface. Photon migration at 830-nm excitation was studied with five bone slices and a 380 μm-thin polytetrafluoroethylene (PTFE) slice placed consecutively between the layers. To optimize Raman signal recovery of the PTFE with increasing depth within the bone stack required a corresponding increase in spatial offset. For example, to sample effectively at 2.2-mm depth within the bone required an optimal SORS offset of 7mm. However, with a 7-mm offset, the maximum accessible penetration depth from which the PTFE signal could be still recovered was 3.7mm. These results provide essential basic information for developing SORS technology for medical diagnostics in general and optimizing sampling through bone tissue, permitting a better understanding of the relationship between the offset and depth of bone assessed, in particular. Potential applications include the detection of chemically specific markers for changes in bone matrix chemistry localized within the tissue and not present in healthy bone.

AB - Spatially offset Raman spectroscopy (SORS) is currently being developed as anin vivo tool for bone disease detection, but to date, information about the interrogated volume as influenced by the light propagation and scattering characteristics of the bone matrix is still limited. This paper seeks to develop our general understanding of the sampling depths of SORS in bone specimens as a function of the applied spatial offset. Equine metacarpal bone was selected as a suitable specimen of compact cortical bone large enough to allow several thin slices (600 μm) to be cut from the dorsal surface. Photon migration at 830-nm excitation was studied with five bone slices and a 380 μm-thin polytetrafluoroethylene (PTFE) slice placed consecutively between the layers. To optimize Raman signal recovery of the PTFE with increasing depth within the bone stack required a corresponding increase in spatial offset. For example, to sample effectively at 2.2-mm depth within the bone required an optimal SORS offset of 7mm. However, with a 7-mm offset, the maximum accessible penetration depth from which the PTFE signal could be still recovered was 3.7mm. These results provide essential basic information for developing SORS technology for medical diagnostics in general and optimizing sampling through bone tissue, permitting a better understanding of the relationship between the offset and depth of bone assessed, in particular. Potential applications include the detection of chemically specific markers for changes in bone matrix chemistry localized within the tissue and not present in healthy bone.

KW - spatially offset Raman spectroscopy (SORS)

KW - bone

KW - photon migration

KW - diffuse scattering

U2 - 10.1002/jrs.4781

DO - 10.1002/jrs.4781

M3 - Journal article

VL - 47

SP - 240

EP - 247

JO - Journal of Raman Spectroscopy

JF - Journal of Raman Spectroscopy

SN - 0377-0486

IS - 2

ER -