Rights statement: This is the peer reviewed version of the following article: Sowoidnich, K., Churchwell, J. H., Buckley, K., Goodship, A. E., Parker, A. W., and Matousek, P. (2016) Photon migration of Raman signal in bone as measured with spatially offset Raman spectroscopy. J. Raman Spectrosc., 47: 240–247. doi: 10.1002/jrs.4781 which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/jrs.4781/abstract This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
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Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Photon migration of Raman signal in bone as measured with spatially offset Raman spectroscopy
AU - Sowoidnich, Kay
AU - Churchwell, John
AU - Kerns, Jemma Gillian
AU - Buckley, Kevin
AU - Goodship, Allen
AU - Parker, Anthony W.
AU - Matousek, Pavel
N1 - This is the peer reviewed version of the following article: Sowoidnich, K., Churchwell, J. H., Buckley, K., Goodship, A. E., Parker, A. W., and Matousek, P. (2016) Photon migration of Raman signal in bone as measured with spatially offset Raman spectroscopy. J. Raman Spectrosc., 47: 240–247. doi: 10.1002/jrs.4781 which has been published in final form at http://onlinelibrary.wiley.com/doi/10.1002/jrs.4781/abstract This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.
PY - 2016/2
Y1 - 2016/2
N2 - Spatially offset Raman spectroscopy (SORS) is currently being developed as anin vivo tool for bone disease detection, but to date, information about the interrogated volume as influenced by the light propagation and scattering characteristics of the bone matrix is still limited. This paper seeks to develop our general understanding of the sampling depths of SORS in bone specimens as a function of the applied spatial offset. Equine metacarpal bone was selected as a suitable specimen of compact cortical bone large enough to allow several thin slices (600 μm) to be cut from the dorsal surface. Photon migration at 830-nm excitation was studied with five bone slices and a 380 μm-thin polytetrafluoroethylene (PTFE) slice placed consecutively between the layers. To optimize Raman signal recovery of the PTFE with increasing depth within the bone stack required a corresponding increase in spatial offset. For example, to sample effectively at 2.2-mm depth within the bone required an optimal SORS offset of 7mm. However, with a 7-mm offset, the maximum accessible penetration depth from which the PTFE signal could be still recovered was 3.7mm. These results provide essential basic information for developing SORS technology for medical diagnostics in general and optimizing sampling through bone tissue, permitting a better understanding of the relationship between the offset and depth of bone assessed, in particular. Potential applications include the detection of chemically specific markers for changes in bone matrix chemistry localized within the tissue and not present in healthy bone.
AB - Spatially offset Raman spectroscopy (SORS) is currently being developed as anin vivo tool for bone disease detection, but to date, information about the interrogated volume as influenced by the light propagation and scattering characteristics of the bone matrix is still limited. This paper seeks to develop our general understanding of the sampling depths of SORS in bone specimens as a function of the applied spatial offset. Equine metacarpal bone was selected as a suitable specimen of compact cortical bone large enough to allow several thin slices (600 μm) to be cut from the dorsal surface. Photon migration at 830-nm excitation was studied with five bone slices and a 380 μm-thin polytetrafluoroethylene (PTFE) slice placed consecutively between the layers. To optimize Raman signal recovery of the PTFE with increasing depth within the bone stack required a corresponding increase in spatial offset. For example, to sample effectively at 2.2-mm depth within the bone required an optimal SORS offset of 7mm. However, with a 7-mm offset, the maximum accessible penetration depth from which the PTFE signal could be still recovered was 3.7mm. These results provide essential basic information for developing SORS technology for medical diagnostics in general and optimizing sampling through bone tissue, permitting a better understanding of the relationship between the offset and depth of bone assessed, in particular. Potential applications include the detection of chemically specific markers for changes in bone matrix chemistry localized within the tissue and not present in healthy bone.
KW - spatially offset Raman spectroscopy (SORS)
KW - bone
KW - photon migration
KW - diffuse scattering
U2 - 10.1002/jrs.4781
DO - 10.1002/jrs.4781
M3 - Journal article
VL - 47
SP - 240
EP - 247
JO - Journal of Raman Spectroscopy
JF - Journal of Raman Spectroscopy
SN - 0377-0486
IS - 2
ER -