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    Rights statement: This is the peer reviewed version of the following article: De Souza, A.P., Wang, Y., Orr, D.J., Carmo‐Silva, E. and Long, S.P. (2020), Photosynthesis across African cassava germplasm is limited by Rubisco and mesophyll conductance at steady state, but by stomatal conductance in fluctuating light. New Phytol, 225: 2498-2512. doi:10.1111/nph.16142 which has been published in final form at https://nph.onlinelibrary.wiley.com/doi/abs/10.1111/nph.16142 This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.

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Photosynthesis across African cassava germplasm is limited by Rubisco and mesophyll conductance at steady-state, but by stomatal conductance in fluctuating light

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Photosynthesis across African cassava germplasm is limited by Rubisco and mesophyll conductance at steady-state, but by stomatal conductance in fluctuating light. / De Souza, Amanda; Yu, Wang; Orr, Douglas John; Carmo-Silva, Ana Elizabete; Long, Stephen.

In: New Phytologist, Vol. 225, No. 6, 31.03.2020, p. 2498-2512.

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@article{73eec695dc3c40018b0d4d452931e6b6,
title = "Photosynthesis across African cassava germplasm is limited by Rubisco and mesophyll conductance at steady-state, but by stomatal conductance in fluctuating light",
abstract = "Sub‐Saharan Africa is projected to see a 55% increase in food demand by 2035, where cassava (Manihot esculenta) is the most planted crop and a major calorie source. Cassava yield has not increased significantly for 13 years. Improvement of genetic yield potential, the basis of the first Green Revolution, could be increased by improving photosynthetic efficiency. First, the factors limiting photosynthesis and their genetic variability within extant germplasm must be understood.Biochemical and diffusive limitations to leaf photosynthetic CO2 uptake under steady‐state and fluctuating light in thirteen farm‐preferred and high‐yielding African cultivars were analyzed. A cassava leaf metabolic model was developed to quantify the value of overcoming limitations to leaf photosynthesis.At steady‐state, in vivo Rubisco activity and mesophyll conductance accounted for 84% of the limitation whereas under non‐steady‐state conditions of shade to sun transition stomatal conductance was the major limitation contributing resulting in an estimated 13% and 5% losses in CO2 uptake and water use efficiency, across a diurnal period. Triose phosphate utilization, while sufficient to support observed rates, would limit improvement in leaf photosynthesis to 33%, unless improved itself.The variation of carbon assimilation among cultivars were three times greater under non‐steady‐state compared to steady‐state, pinpointing important overlooked breeding targets for improved photosynthetic efficiency in cassava.",
keywords = "cassava breeding, food security, genetic engineering, Manihot esculenta, photosynthesis, Rubisco activase, Sub‐Saharan Africa, crop yield",
author = "{De Souza}, Amanda and Wang Yu and Orr, {Douglas John} and Carmo-Silva, {Ana Elizabete} and Stephen Long",
note = "This is the peer reviewed version of the following article: De Souza, A.P., Wang, Y., Orr, D.J., Carmo‐Silva, E. and Long, S.P. (2020), Photosynthesis across African cassava germplasm is limited by Rubisco and mesophyll conductance at steady state, but by stomatal conductance in fluctuating light. New Phytol, 225: 2498-2512. doi:10.1111/nph.16142 which has been published in final form at https://nph.onlinelibrary.wiley.com/doi/abs/10.1111/nph.16142 This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.",
year = "2020",
month = mar
day = "31",
doi = "10.1111/nph.16142",
language = "English",
volume = "225",
pages = "2498--2512",
journal = "New Phytologist",
issn = "0028-646X",
publisher = "Wiley",
number = "6",

}

RIS

TY - JOUR

T1 - Photosynthesis across African cassava germplasm is limited by Rubisco and mesophyll conductance at steady-state, but by stomatal conductance in fluctuating light

AU - De Souza, Amanda

AU - Yu, Wang

AU - Orr, Douglas John

AU - Carmo-Silva, Ana Elizabete

AU - Long, Stephen

N1 - This is the peer reviewed version of the following article: De Souza, A.P., Wang, Y., Orr, D.J., Carmo‐Silva, E. and Long, S.P. (2020), Photosynthesis across African cassava germplasm is limited by Rubisco and mesophyll conductance at steady state, but by stomatal conductance in fluctuating light. New Phytol, 225: 2498-2512. doi:10.1111/nph.16142 which has been published in final form at https://nph.onlinelibrary.wiley.com/doi/abs/10.1111/nph.16142 This article may be used for non-commercial purposes in accordance With Wiley Terms and Conditions for self-archiving.

PY - 2020/3/31

Y1 - 2020/3/31

N2 - Sub‐Saharan Africa is projected to see a 55% increase in food demand by 2035, where cassava (Manihot esculenta) is the most planted crop and a major calorie source. Cassava yield has not increased significantly for 13 years. Improvement of genetic yield potential, the basis of the first Green Revolution, could be increased by improving photosynthetic efficiency. First, the factors limiting photosynthesis and their genetic variability within extant germplasm must be understood.Biochemical and diffusive limitations to leaf photosynthetic CO2 uptake under steady‐state and fluctuating light in thirteen farm‐preferred and high‐yielding African cultivars were analyzed. A cassava leaf metabolic model was developed to quantify the value of overcoming limitations to leaf photosynthesis.At steady‐state, in vivo Rubisco activity and mesophyll conductance accounted for 84% of the limitation whereas under non‐steady‐state conditions of shade to sun transition stomatal conductance was the major limitation contributing resulting in an estimated 13% and 5% losses in CO2 uptake and water use efficiency, across a diurnal period. Triose phosphate utilization, while sufficient to support observed rates, would limit improvement in leaf photosynthesis to 33%, unless improved itself.The variation of carbon assimilation among cultivars were three times greater under non‐steady‐state compared to steady‐state, pinpointing important overlooked breeding targets for improved photosynthetic efficiency in cassava.

AB - Sub‐Saharan Africa is projected to see a 55% increase in food demand by 2035, where cassava (Manihot esculenta) is the most planted crop and a major calorie source. Cassava yield has not increased significantly for 13 years. Improvement of genetic yield potential, the basis of the first Green Revolution, could be increased by improving photosynthetic efficiency. First, the factors limiting photosynthesis and their genetic variability within extant germplasm must be understood.Biochemical and diffusive limitations to leaf photosynthetic CO2 uptake under steady‐state and fluctuating light in thirteen farm‐preferred and high‐yielding African cultivars were analyzed. A cassava leaf metabolic model was developed to quantify the value of overcoming limitations to leaf photosynthesis.At steady‐state, in vivo Rubisco activity and mesophyll conductance accounted for 84% of the limitation whereas under non‐steady‐state conditions of shade to sun transition stomatal conductance was the major limitation contributing resulting in an estimated 13% and 5% losses in CO2 uptake and water use efficiency, across a diurnal period. Triose phosphate utilization, while sufficient to support observed rates, would limit improvement in leaf photosynthesis to 33%, unless improved itself.The variation of carbon assimilation among cultivars were three times greater under non‐steady‐state compared to steady‐state, pinpointing important overlooked breeding targets for improved photosynthetic efficiency in cassava.

KW - cassava breeding

KW - food security

KW - genetic engineering

KW - Manihot esculenta

KW - photosynthesis

KW - Rubisco activase

KW - Sub‐Saharan Africa

KW - crop yield

U2 - 10.1111/nph.16142

DO - 10.1111/nph.16142

M3 - Journal article

VL - 225

SP - 2498

EP - 2512

JO - New Phytologist

JF - New Phytologist

SN - 0028-646X

IS - 6

ER -