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Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels

Research output: Contribution to journalJournal article

  • Jana Lišková
  • Timothy E.L. Douglas
  • Robbe Wijnants
  • Sangram Keshari Samal
  • Ana C. Mendes
  • Ioannis Chronakis
  • Lucie Bačáková
  • Andre G. Skirtach
<mark>Journal publication date</mark>1/03/2018
<mark>Journal</mark>Materials Letters
Number of pages4
Pages (from-to)186-189
Early online date5/12/17
<mark>Original language</mark>English


Hydrogels mineralized with calcium phosphate (CaP) are increasingly popular bone regeneration biomaterials. Mineralization can be achieved by phosphatase enzyme incorporation and incubation in calcium glycerophosphate (CaGP). Gellan gum (GG) hydrogels containing the enzyme phytase and chitosan oligomer were mineralized in CaGP solution and characterized with human osteoblast-like MG63 cells and adipose tissue-derived stem cells (ADSC). Phytase induced CaP formation. Chitosan concentration determined mineralization extent and hydrogel mechanical reinforcement. Phytase-induced mineralization promoted MG63 adhesion and proliferation, especially in the presence of chitosan, and was non-toxic to MG63 cells (with and without chitosan). ADSC adhesion and proliferation were poor without mineralization. Chitosan did not affect ADSC osteogenic differentiation.

Bibliographic note

This is the author’s version of a work that was accepted for publication in Materials Letters. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Materials Letters, 214, 2018 DOI: 10.1016/j.matlet.2017.12.004