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    Rights statement: This is the author’s version of a work that was accepted for publication in Materials Letters. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Materials Letters, 214, 2018 DOI: 10.1016/j.matlet.2017.12.004

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Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels

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Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels. / Lišková, Jana; Douglas, Timothy E.L.; Wijnants, Robbe; Samal, Sangram Keshari; Mendes, Ana C.; Chronakis, Ioannis; Bačáková, Lucie; Skirtach, Andre G.

In: Materials Letters, Vol. 214, 01.03.2018, p. 186-189.

Research output: Contribution to journalJournal article

Harvard

Lišková, J, Douglas, TEL, Wijnants, R, Samal, SK, Mendes, AC, Chronakis, I, Bačáková, L & Skirtach, AG 2018, 'Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels' Materials Letters, vol. 214, pp. 186-189. DOI: 10.1016/j.matlet.2017.12.004

APA

Lišková, J., Douglas, T. E. L., Wijnants, R., Samal, S. K., Mendes, A. C., Chronakis, I., ... Skirtach, A. G. (2018). Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels. DOI: 10.1016/j.matlet.2017.12.004

Vancouver

Lišková J, Douglas TEL, Wijnants R, Samal SK, Mendes AC, Chronakis I et al. Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels. Materials Letters. 2018 Mar 1;214:186-189. Available from, DOI: 10.1016/j.matlet.2017.12.004

Author

Lišková, Jana ; Douglas, Timothy E.L. ; Wijnants, Robbe ; Samal, Sangram Keshari ; Mendes, Ana C. ; Chronakis, Ioannis ; Bačáková, Lucie ; Skirtach, Andre G./ Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels. In: Materials Letters. 2018 ; Vol. 214. pp. 186-189

Bibtex

@article{e56a2dd0ed4745e396a6dc425d3dd5cf,
title = "Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels",
abstract = "Hydrogels mineralized with calcium phosphate (CaP) are increasingly popular bone regeneration biomaterials. Mineralization can be achieved by phosphatase enzyme incorporation and incubation in calcium glycerophosphate (CaGP). Gellan gum (GG) hydrogels containing the enzyme phytase and chitosan oligomer were mineralized in CaGP solution and characterized with human osteoblast-like MG63 cells and adipose tissue-derived stem cells (ADSC). Phytase induced CaP formation. Chitosan concentration determined mineralization extent and hydrogel mechanical reinforcement. Phytase-induced mineralization promoted MG63 adhesion and proliferation, especially in the presence of chitosan, and was non-toxic to MG63 cells (with and without chitosan). ADSC adhesion and proliferation were poor without mineralization. Chitosan did not affect ADSC osteogenic differentiation.",
keywords = "Biomaterials, Biomimetic, Composite materials",
author = "Jana Liškov{\'a} and Douglas, {Timothy E.L.} and Robbe Wijnants and Samal, {Sangram Keshari} and Mendes, {Ana C.} and Ioannis Chronakis and Lucie Bač{\'a}kov{\'a} and Skirtach, {Andre G.}",
note = "This is the author’s version of a work that was accepted for publication in Materials Letters. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Materials Letters, 214, 2018 DOI: 10.1016/j.matlet.2017.12.004",
year = "2018",
month = "3",
day = "1",
doi = "10.1016/j.matlet.2017.12.004",
language = "English",
volume = "214",
pages = "186--189",
journal = "Materials Letters",
issn = "0167-577X",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - Phytase-mediated enzymatic mineralization of chitosan-enriched hydrogels

AU - Lišková,Jana

AU - Douglas,Timothy E.L.

AU - Wijnants,Robbe

AU - Samal,Sangram Keshari

AU - Mendes,Ana C.

AU - Chronakis,Ioannis

AU - Bačáková,Lucie

AU - Skirtach,Andre G.

N1 - This is the author’s version of a work that was accepted for publication in Materials Letters. Changes resulting from the publishing process, such as peer review, editing, corrections, structural formatting, and other quality control mechanisms may not be reflected in this document. Changes may have been made to this work since it was submitted for publication. A definitive version was subsequently published in Materials Letters, 214, 2018 DOI: 10.1016/j.matlet.2017.12.004

PY - 2018/3/1

Y1 - 2018/3/1

N2 - Hydrogels mineralized with calcium phosphate (CaP) are increasingly popular bone regeneration biomaterials. Mineralization can be achieved by phosphatase enzyme incorporation and incubation in calcium glycerophosphate (CaGP). Gellan gum (GG) hydrogels containing the enzyme phytase and chitosan oligomer were mineralized in CaGP solution and characterized with human osteoblast-like MG63 cells and adipose tissue-derived stem cells (ADSC). Phytase induced CaP formation. Chitosan concentration determined mineralization extent and hydrogel mechanical reinforcement. Phytase-induced mineralization promoted MG63 adhesion and proliferation, especially in the presence of chitosan, and was non-toxic to MG63 cells (with and without chitosan). ADSC adhesion and proliferation were poor without mineralization. Chitosan did not affect ADSC osteogenic differentiation.

AB - Hydrogels mineralized with calcium phosphate (CaP) are increasingly popular bone regeneration biomaterials. Mineralization can be achieved by phosphatase enzyme incorporation and incubation in calcium glycerophosphate (CaGP). Gellan gum (GG) hydrogels containing the enzyme phytase and chitosan oligomer were mineralized in CaGP solution and characterized with human osteoblast-like MG63 cells and adipose tissue-derived stem cells (ADSC). Phytase induced CaP formation. Chitosan concentration determined mineralization extent and hydrogel mechanical reinforcement. Phytase-induced mineralization promoted MG63 adhesion and proliferation, especially in the presence of chitosan, and was non-toxic to MG63 cells (with and without chitosan). ADSC adhesion and proliferation were poor without mineralization. Chitosan did not affect ADSC osteogenic differentiation.

KW - Biomaterials

KW - Biomimetic

KW - Composite materials

U2 - 10.1016/j.matlet.2017.12.004

DO - 10.1016/j.matlet.2017.12.004

M3 - Journal article

VL - 214

SP - 186

EP - 189

JO - Materials Letters

T2 - Materials Letters

JF - Materials Letters

SN - 0167-577X

ER -