Final published version, 2.43 MB, PDF document
Available under license: CC BY-NC: Creative Commons Attribution-NonCommercial 4.0 International License
Final published version
Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
}
TY - JOUR
T1 - Phytase-producing Bacillus sp. inoculation increases phosphorus availability in cattle manure
AU - Blackburn, Daniel Menezes
AU - Inostroza, N. G.
AU - Gianfreda, L.
AU - Greiner, R.
AU - Mora, M. L.
AU - Jorquera, M. A.
PY - 2016/3
Y1 - 2016/3
N2 - Organic wastes rich in phosphorus (P) are considered an alternative to decrease the dependence on chemical P fertilization in crops and pastures. Microbial inoculants are being studied as a tool to increase plant P availability in organic wastes. In this study, we explore the effect of inoculation with Bacillus sp. MQH-19 (a native phytase-producing bacterium) on the release of inorganic phosphorus (Pi) in cattle manure with low available P but a high total P content. Bacteria inoculation resulted in a higher release of Pi (8% in NaHCÜ3 and 13% in NaOH-EDTA extracts) compared with that of uninoculated manure (0.7% in NaHCÜ3 and 0.1% in NaOH-EDTA extracts). However, a greater amount of Pi was released in inoculated manure supplemented with phytate (47% in NaHCÜ3 and 117% in NaOH-EDTA extracts) compared with that of uninoculated manure supplemented with phytate (30% in NaHCÜ3 and 15% in NaOH-EDTA extracts). In addition, the use of denaturing gradient gel electrophoresis (DGGE) and quantitative PCR (qPCR) revealed that the bacterial community structure in manure was affected by inoculation and that the prevalence of Bacillus sp. MQH-19 decreased during incubation (6 days). This study demonstrates that Pi availability in cattle manure can be increased by phytase-producing bacteria inoculation. Phytase-producing bacteria inoculation might represent an attractive strategy to increase P availability in agricultural wastes, which are used as organic fertilizers in crops and pastures.
AB - Organic wastes rich in phosphorus (P) are considered an alternative to decrease the dependence on chemical P fertilization in crops and pastures. Microbial inoculants are being studied as a tool to increase plant P availability in organic wastes. In this study, we explore the effect of inoculation with Bacillus sp. MQH-19 (a native phytase-producing bacterium) on the release of inorganic phosphorus (Pi) in cattle manure with low available P but a high total P content. Bacteria inoculation resulted in a higher release of Pi (8% in NaHCÜ3 and 13% in NaOH-EDTA extracts) compared with that of uninoculated manure (0.7% in NaHCÜ3 and 0.1% in NaOH-EDTA extracts). However, a greater amount of Pi was released in inoculated manure supplemented with phytate (47% in NaHCÜ3 and 117% in NaOH-EDTA extracts) compared with that of uninoculated manure supplemented with phytate (30% in NaHCÜ3 and 15% in NaOH-EDTA extracts). In addition, the use of denaturing gradient gel electrophoresis (DGGE) and quantitative PCR (qPCR) revealed that the bacterial community structure in manure was affected by inoculation and that the prevalence of Bacillus sp. MQH-19 decreased during incubation (6 days). This study demonstrates that Pi availability in cattle manure can be increased by phytase-producing bacteria inoculation. Phytase-producing bacteria inoculation might represent an attractive strategy to increase P availability in agricultural wastes, which are used as organic fertilizers in crops and pastures.
KW - Bacillus
KW - cattle manure
KW - phytate
KW - phytase-producing bacteria
KW - phosphorus
U2 - 10.4067/S0718-95162016005000016
DO - 10.4067/S0718-95162016005000016
M3 - Journal article
VL - 16
SP - 200
EP - 210
JO - Journal of Soil Science and Plant Nutrition
JF - Journal of Soil Science and Plant Nutrition
SN - 0718-9508
IS - 1
ER -