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Polymorphic microsatellite repeats are not conserved between Leishmania donovani and Leishmania major

Research output: Contribution to journalJournal article

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  • Manal Jamjoom
  • R W Ashford
  • Paul Bates
  • Stephen J Kemp
  • Harry A Noyes
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<mark>Journal publication date</mark>06/2002
<mark>Journal</mark>Molecular Ecology Notes
Issue number2
Volume2
Number of pages3
Pages (from-to)104-106
Publication statusPublished
Original languageEnglish

Abstract

Thirteen sets of polymerase chain reaction (PCR) primers were designed to amplify microsatellite loci identified in the genome sequence of Leishmania major. Polymorphisms were detected in L. major at all loci. In Leishmania donovani only two of these loci were informative for classification purposes with this data set. The PCR products of all loci from one L. donovani strain were sequenced and it was found that the number of repeats in the microsatellite loci were either substantially reduced with respect to L. major or absent altogether. Consequently it is unlikely to be possible to use the genome sequence of L. major to identify polymorphic microsatellite loci in other Leishmania species.