Spongy scaffolds containing nano-carbonatcd hydroxyapatitc (nCHA), with composition Ca10(P04)6- y(C03)y(0H)2,with y=2,4, were prepared by the Iyophilization method. Different polymers, natural gelatin (G), chitosan (CS) and polyvinyl alcohol (PVA), alone or in combination, were used to make their compositcs with nCHA. All the samples were characterized by (XRD), (SEM), (FT-IR) techniques. P3rticlc size and surface area were determined by BET surfacc area analysis. The nCHA containing scaffolds mediated cytotoxicity (MTT assay) and cell attachment studies (SEM) were carried out on rat bone marrow derived mesenchymal stem cell (MSC). SEM studies revealed varying microstmctures for pore sizes, shapes and pore interconnectivicy. All the scaffolds were found to be highly porous, porosities beyond 70%. It is observed that the scafTold morphology changes significantly for one nCHA-'polymer combination to the other. Furthermore we investigated the biocompatibility of these scaffolds by performing cell viability and cell attachment tests. Our results indicated evident cell attachment on all the materials and also no significant cytotoxicity was observed in cells grown with these materials compared with control. The preparation of spongy scaffolds and their biocompatibility augment their potential use in tissue engineering and biomedical applications. © 2015 by TechConnect. All rights reserved.