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Production of chitosan PVA PCL hydrogels to bind heparin and induce angiogenesis

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Production of chitosan PVA PCL hydrogels to bind heparin and induce angiogenesis. / Yar, M.; Gigliobianco, G.; Shahzadi, L.; Dew, L.; Siddiqi, S.A.; Khan, A.F.; Chaudhry, A.A.; Rehman, I.U.; MacNeil, S.

In: International Journal of Polymeric Materials and Polymeric Biomaterials, Vol. 65, No. 9, 01.09.2016, p. 466-476.

Research output: Contribution to journalJournal article

Harvard

Yar, M, Gigliobianco, G, Shahzadi, L, Dew, L, Siddiqi, SA, Khan, AF, Chaudhry, AA, Rehman, IU & MacNeil, S 2016, 'Production of chitosan PVA PCL hydrogels to bind heparin and induce angiogenesis', International Journal of Polymeric Materials and Polymeric Biomaterials, vol. 65, no. 9, pp. 466-476. https://doi.org/10.1080/00914037.2015.1129959

APA

Yar, M., Gigliobianco, G., Shahzadi, L., Dew, L., Siddiqi, S. A., Khan, A. F., Chaudhry, A. A., Rehman, I. U., & MacNeil, S. (2016). Production of chitosan PVA PCL hydrogels to bind heparin and induce angiogenesis. International Journal of Polymeric Materials and Polymeric Biomaterials, 65(9), 466-476. https://doi.org/10.1080/00914037.2015.1129959

Vancouver

Yar M, Gigliobianco G, Shahzadi L, Dew L, Siddiqi SA, Khan AF et al. Production of chitosan PVA PCL hydrogels to bind heparin and induce angiogenesis. International Journal of Polymeric Materials and Polymeric Biomaterials. 2016 Sep 1;65(9):466-476. https://doi.org/10.1080/00914037.2015.1129959

Author

Yar, M. ; Gigliobianco, G. ; Shahzadi, L. ; Dew, L. ; Siddiqi, S.A. ; Khan, A.F. ; Chaudhry, A.A. ; Rehman, I.U. ; MacNeil, S. / Production of chitosan PVA PCL hydrogels to bind heparin and induce angiogenesis. In: International Journal of Polymeric Materials and Polymeric Biomaterials. 2016 ; Vol. 65, No. 9. pp. 466-476.

Bibtex

@article{a128f3fbe73c4c44be8bc45b31e7db4e,
title = "Production of chitosan PVA PCL hydrogels to bind heparin and induce angiogenesis",
abstract = "New blood vessel formation is an essential part of wound healing to provide cells with the nutrients and oxygen for their survival. Many nonhealing ulcers fail to heal because of poor blood supply and skin grafts will also fail to take on poorly vascularized wound beds. There is a real need for proangiogenic biomaterials to assist wound healing. In vivo heparin binds proangiogenic growth factors and helps regulate new blood vessel formation, hence heparin containing biomaterials are attractive. To achieve a hydrogel with high heparin binding capacity a composite of chitosan, poly(vinyl alcohol) (PVA) and polycaprolactone (PCL) was produced. Chitosan is a biodegradable natural polymer with great potential for biomedical applications due to its biocompatibility, high charge density and nontoxicity. PVA is biocompatible and nontoxic with good chemical stability, film-forming ability, and high hydrophilicity. PCL has physicochemical and mechanical properties comparable to those of the biological tissues and due its hydrophilic nature helps in the sustained release of drugs. Accordingly in this study we explored a range of PCL concentrations from 4% to 16% added to hydrogels composed of chitosan and PVA. Heparin was blended into the polymer mixture and the nanoporous structure was created by freeze-drying the PCL hydrogel. The physical properties of the hydrogels were evaluated by Fourier transform infrared spectroscopy (FTIR) and XPS confirmed the presence of sulfur on the surface of the hydrogels. Their porous morphology was investigated by scanning electron microscope (SEM). The Chick Chorionic Allantoic Membrane (CAM) assay was used to study the angiogenic potential of these materials and histology (H&E and Goldner trochome) was used to confirm the presence of new blood vessels inside the hydrogels. We report that the addition of 8% PCL to the hydrogels gave porous structures containing heparin, which significantly increased new blood vessel formation into the hydrogels. These hydrogels offer a new approach to biomaterials, which could be added to wounds to improve vascularization.",
keywords = "Angiogenesis, chitosan, dhydrogel, heparin, wound dressing, Bins, Biocompatibility, Biodegradable polymers, Biomaterials, Biomechanics, Blood vessels, Chemical stability, Chitin, Chitosan, Fourier transform infrared spectroscopy, Functional polymers, Histology, Hydrophilicity, Medical applications, Polycaprolactone, Polysaccharides, Polyvinyl alcohols, Scanning electron microscopy, Tissue, Biomedical applications, Film-forming abilities, Physico-chemical and mechanical properties, Poly (vinyl alcohol) (PVA), Wound dressings, Hydrogels",
author = "M. Yar and G. Gigliobianco and L. Shahzadi and L. Dew and S.A. Siddiqi and A.F. Khan and A.A. Chaudhry and I.U. Rehman and S. MacNeil",
year = "2016",
month = sep,
day = "1",
doi = "10.1080/00914037.2015.1129959",
language = "English",
volume = "65",
pages = "466--476",
journal = "International Journal of Polymeric Materials and Polymeric Biomaterials",
issn = "0091-4037",
publisher = "Taylor and Francis Inc.",
number = "9",

}

RIS

TY - JOUR

T1 - Production of chitosan PVA PCL hydrogels to bind heparin and induce angiogenesis

AU - Yar, M.

AU - Gigliobianco, G.

AU - Shahzadi, L.

AU - Dew, L.

AU - Siddiqi, S.A.

AU - Khan, A.F.

AU - Chaudhry, A.A.

AU - Rehman, I.U.

AU - MacNeil, S.

PY - 2016/9/1

Y1 - 2016/9/1

N2 - New blood vessel formation is an essential part of wound healing to provide cells with the nutrients and oxygen for their survival. Many nonhealing ulcers fail to heal because of poor blood supply and skin grafts will also fail to take on poorly vascularized wound beds. There is a real need for proangiogenic biomaterials to assist wound healing. In vivo heparin binds proangiogenic growth factors and helps regulate new blood vessel formation, hence heparin containing biomaterials are attractive. To achieve a hydrogel with high heparin binding capacity a composite of chitosan, poly(vinyl alcohol) (PVA) and polycaprolactone (PCL) was produced. Chitosan is a biodegradable natural polymer with great potential for biomedical applications due to its biocompatibility, high charge density and nontoxicity. PVA is biocompatible and nontoxic with good chemical stability, film-forming ability, and high hydrophilicity. PCL has physicochemical and mechanical properties comparable to those of the biological tissues and due its hydrophilic nature helps in the sustained release of drugs. Accordingly in this study we explored a range of PCL concentrations from 4% to 16% added to hydrogels composed of chitosan and PVA. Heparin was blended into the polymer mixture and the nanoporous structure was created by freeze-drying the PCL hydrogel. The physical properties of the hydrogels were evaluated by Fourier transform infrared spectroscopy (FTIR) and XPS confirmed the presence of sulfur on the surface of the hydrogels. Their porous morphology was investigated by scanning electron microscope (SEM). The Chick Chorionic Allantoic Membrane (CAM) assay was used to study the angiogenic potential of these materials and histology (H&E and Goldner trochome) was used to confirm the presence of new blood vessels inside the hydrogels. We report that the addition of 8% PCL to the hydrogels gave porous structures containing heparin, which significantly increased new blood vessel formation into the hydrogels. These hydrogels offer a new approach to biomaterials, which could be added to wounds to improve vascularization.

AB - New blood vessel formation is an essential part of wound healing to provide cells with the nutrients and oxygen for their survival. Many nonhealing ulcers fail to heal because of poor blood supply and skin grafts will also fail to take on poorly vascularized wound beds. There is a real need for proangiogenic biomaterials to assist wound healing. In vivo heparin binds proangiogenic growth factors and helps regulate new blood vessel formation, hence heparin containing biomaterials are attractive. To achieve a hydrogel with high heparin binding capacity a composite of chitosan, poly(vinyl alcohol) (PVA) and polycaprolactone (PCL) was produced. Chitosan is a biodegradable natural polymer with great potential for biomedical applications due to its biocompatibility, high charge density and nontoxicity. PVA is biocompatible and nontoxic with good chemical stability, film-forming ability, and high hydrophilicity. PCL has physicochemical and mechanical properties comparable to those of the biological tissues and due its hydrophilic nature helps in the sustained release of drugs. Accordingly in this study we explored a range of PCL concentrations from 4% to 16% added to hydrogels composed of chitosan and PVA. Heparin was blended into the polymer mixture and the nanoporous structure was created by freeze-drying the PCL hydrogel. The physical properties of the hydrogels were evaluated by Fourier transform infrared spectroscopy (FTIR) and XPS confirmed the presence of sulfur on the surface of the hydrogels. Their porous morphology was investigated by scanning electron microscope (SEM). The Chick Chorionic Allantoic Membrane (CAM) assay was used to study the angiogenic potential of these materials and histology (H&E and Goldner trochome) was used to confirm the presence of new blood vessels inside the hydrogels. We report that the addition of 8% PCL to the hydrogels gave porous structures containing heparin, which significantly increased new blood vessel formation into the hydrogels. These hydrogels offer a new approach to biomaterials, which could be added to wounds to improve vascularization.

KW - Angiogenesis

KW - chitosan

KW - dhydrogel

KW - heparin

KW - wound dressing

KW - Bins

KW - Biocompatibility

KW - Biodegradable polymers

KW - Biomaterials

KW - Biomechanics

KW - Blood vessels

KW - Chemical stability

KW - Chitin

KW - Chitosan

KW - Fourier transform infrared spectroscopy

KW - Functional polymers

KW - Histology

KW - Hydrophilicity

KW - Medical applications

KW - Polycaprolactone

KW - Polysaccharides

KW - Polyvinyl alcohols

KW - Scanning electron microscopy

KW - Tissue

KW - Biomedical applications

KW - Film-forming abilities

KW - Physico-chemical and mechanical properties

KW - Poly (vinyl alcohol) (PVA)

KW - Wound dressings

KW - Hydrogels

U2 - 10.1080/00914037.2015.1129959

DO - 10.1080/00914037.2015.1129959

M3 - Journal article

VL - 65

SP - 466

EP - 476

JO - International Journal of Polymeric Materials and Polymeric Biomaterials

JF - International Journal of Polymeric Materials and Polymeric Biomaterials

SN - 0091-4037

IS - 9

ER -