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Protective role of DNJ-27/ERdj5 in Caenorhabditis elegans models of human neurodegenerative diseases

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Protective role of DNJ-27/ERdj5 in Caenorhabditis elegans models of human neurodegenerative diseases. / Muñoz-Lobato, Fernando; Rodríguez-Palero, María Jesús; Naranjo-Galindo, Francisco José; Shephard, Freya; Gaffney, Christopher J; Szewczyk, Nathaniel J; Hamamichi, Shusei; Caldwell, Kim A; Caldwell, Guy A; Link, Chris D; Miranda-Vizuete, Antonio.

In: Antioxidants and Redox Signaling, Vol. 20, No. 2, 10.01.2014, p. 217-235.

Research output: Contribution to journalJournal article

Harvard

Muñoz-Lobato, F, Rodríguez-Palero, MJ, Naranjo-Galindo, FJ, Shephard, F, Gaffney, CJ, Szewczyk, NJ, Hamamichi, S, Caldwell, KA, Caldwell, GA, Link, CD & Miranda-Vizuete, A 2014, 'Protective role of DNJ-27/ERdj5 in Caenorhabditis elegans models of human neurodegenerative diseases', Antioxidants and Redox Signaling, vol. 20, no. 2, pp. 217-235. https://doi.org/10.1089/ars.2012.5051

APA

Muñoz-Lobato, F., Rodríguez-Palero, M. J., Naranjo-Galindo, F. J., Shephard, F., Gaffney, C. J., Szewczyk, N. J., Hamamichi, S., Caldwell, K. A., Caldwell, G. A., Link, C. D., & Miranda-Vizuete, A. (2014). Protective role of DNJ-27/ERdj5 in Caenorhabditis elegans models of human neurodegenerative diseases. Antioxidants and Redox Signaling, 20(2), 217-235. https://doi.org/10.1089/ars.2012.5051

Vancouver

Muñoz-Lobato F, Rodríguez-Palero MJ, Naranjo-Galindo FJ, Shephard F, Gaffney CJ, Szewczyk NJ et al. Protective role of DNJ-27/ERdj5 in Caenorhabditis elegans models of human neurodegenerative diseases. Antioxidants and Redox Signaling. 2014 Jan 10;20(2):217-235. https://doi.org/10.1089/ars.2012.5051

Author

Muñoz-Lobato, Fernando ; Rodríguez-Palero, María Jesús ; Naranjo-Galindo, Francisco José ; Shephard, Freya ; Gaffney, Christopher J ; Szewczyk, Nathaniel J ; Hamamichi, Shusei ; Caldwell, Kim A ; Caldwell, Guy A ; Link, Chris D ; Miranda-Vizuete, Antonio. / Protective role of DNJ-27/ERdj5 in Caenorhabditis elegans models of human neurodegenerative diseases. In: Antioxidants and Redox Signaling. 2014 ; Vol. 20, No. 2. pp. 217-235.

Bibtex

@article{ce44623552a84ed19e6958155d4b1e68,
title = "Protective role of DNJ-27/ERdj5 in Caenorhabditis elegans models of human neurodegenerative diseases",
abstract = "AIMS: Cells have developed quality control systems for protection against proteotoxicity. Misfolded and aggregation-prone proteins, which are behind the initiation and progression of many neurodegenerative diseases (ND), are known to challenge the proteostasis network of the cells. We aimed to explore the role of DNJ-27/ERdj5, an endoplasmic reticulum (ER)-resident thioredoxin protein required as a disulfide reductase for the degradation of misfolded proteins, in well-established Caenorhabditis elegans models of Alzheimer, Parkinson and Huntington diseases.RESULTS: We demonstrate that DNJ-27 is an ER luminal protein and that its expression is induced upon ER stress via IRE-1/XBP-1. When dnj-27 expression is downregulated by RNA interference we find an increase in the aggregation and associated pathological phenotypes (paralysis and motility impairment) caused by human β-amyloid peptide (Aβ), α-synuclein (α-syn) and polyglutamine (polyQ) proteins. In turn, DNJ-27 overexpression ameliorates these deleterious phenotypes. Surprisingly, despite being an ER-resident protein, we show that dnj-27 downregulation alters cytoplasmic protein homeostasis and causes mitochondrial fragmentation. We further demonstrate that DNJ-27 overexpression substantially protects against the mitochondrial fragmentation caused by human Aβ and α-syn peptides in these worm models.INNOVATION: We identify C. elegans dnj-27 as a novel protective gene for the toxicity associated with the expression of human Aβ, α-syn and polyQ proteins, implying a protective role of ERdj5 in Alzheimer, Parkinson and Huntington diseases.CONCLUSION: Our data support a scenario where the levels of DNJ-27/ERdj5 in the ER impact cytoplasmic protein homeostasis and the integrity of the mitochondrial network which might underlie its protective effects in models of proteotoxicity associated to human ND.",
keywords = "Amyloid beta-Peptides, Animals, Animals, Genetically Modified, Autophagy, Caenorhabditis elegans, Caenorhabditis elegans Proteins, Disease Models, Animal, Endoplasmic Reticulum-Associated Degradation, Gene Expression, Gene Expression Regulation, HSP40 Heat-Shock Proteins, Humans, Mitochondria, Molecular Chaperones, Neurodegenerative Diseases, Peptides, Phenotype, Proteasome Endopeptidase Complex, Proteolysis, RNA Interference, alpha-Synuclein, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't",
author = "Fernando Mu{\~n}oz-Lobato and Rodr{\'i}guez-Palero, {Mar{\'i}a Jes{\'u}s} and Naranjo-Galindo, {Francisco Jos{\'e}} and Freya Shephard and Gaffney, {Christopher J} and Szewczyk, {Nathaniel J} and Shusei Hamamichi and Caldwell, {Kim A} and Caldwell, {Guy A} and Link, {Chris D} and Antonio Miranda-Vizuete",
year = "2014",
month = jan
day = "10",
doi = "10.1089/ars.2012.5051",
language = "English",
volume = "20",
pages = "217--235",
journal = "Antioxidants and Redox Signaling",
issn = "1523-0864",
publisher = "Mary Ann Liebert Inc.",
number = "2",

}

RIS

TY - JOUR

T1 - Protective role of DNJ-27/ERdj5 in Caenorhabditis elegans models of human neurodegenerative diseases

AU - Muñoz-Lobato, Fernando

AU - Rodríguez-Palero, María Jesús

AU - Naranjo-Galindo, Francisco José

AU - Shephard, Freya

AU - Gaffney, Christopher J

AU - Szewczyk, Nathaniel J

AU - Hamamichi, Shusei

AU - Caldwell, Kim A

AU - Caldwell, Guy A

AU - Link, Chris D

AU - Miranda-Vizuete, Antonio

PY - 2014/1/10

Y1 - 2014/1/10

N2 - AIMS: Cells have developed quality control systems for protection against proteotoxicity. Misfolded and aggregation-prone proteins, which are behind the initiation and progression of many neurodegenerative diseases (ND), are known to challenge the proteostasis network of the cells. We aimed to explore the role of DNJ-27/ERdj5, an endoplasmic reticulum (ER)-resident thioredoxin protein required as a disulfide reductase for the degradation of misfolded proteins, in well-established Caenorhabditis elegans models of Alzheimer, Parkinson and Huntington diseases.RESULTS: We demonstrate that DNJ-27 is an ER luminal protein and that its expression is induced upon ER stress via IRE-1/XBP-1. When dnj-27 expression is downregulated by RNA interference we find an increase in the aggregation and associated pathological phenotypes (paralysis and motility impairment) caused by human β-amyloid peptide (Aβ), α-synuclein (α-syn) and polyglutamine (polyQ) proteins. In turn, DNJ-27 overexpression ameliorates these deleterious phenotypes. Surprisingly, despite being an ER-resident protein, we show that dnj-27 downregulation alters cytoplasmic protein homeostasis and causes mitochondrial fragmentation. We further demonstrate that DNJ-27 overexpression substantially protects against the mitochondrial fragmentation caused by human Aβ and α-syn peptides in these worm models.INNOVATION: We identify C. elegans dnj-27 as a novel protective gene for the toxicity associated with the expression of human Aβ, α-syn and polyQ proteins, implying a protective role of ERdj5 in Alzheimer, Parkinson and Huntington diseases.CONCLUSION: Our data support a scenario where the levels of DNJ-27/ERdj5 in the ER impact cytoplasmic protein homeostasis and the integrity of the mitochondrial network which might underlie its protective effects in models of proteotoxicity associated to human ND.

AB - AIMS: Cells have developed quality control systems for protection against proteotoxicity. Misfolded and aggregation-prone proteins, which are behind the initiation and progression of many neurodegenerative diseases (ND), are known to challenge the proteostasis network of the cells. We aimed to explore the role of DNJ-27/ERdj5, an endoplasmic reticulum (ER)-resident thioredoxin protein required as a disulfide reductase for the degradation of misfolded proteins, in well-established Caenorhabditis elegans models of Alzheimer, Parkinson and Huntington diseases.RESULTS: We demonstrate that DNJ-27 is an ER luminal protein and that its expression is induced upon ER stress via IRE-1/XBP-1. When dnj-27 expression is downregulated by RNA interference we find an increase in the aggregation and associated pathological phenotypes (paralysis and motility impairment) caused by human β-amyloid peptide (Aβ), α-synuclein (α-syn) and polyglutamine (polyQ) proteins. In turn, DNJ-27 overexpression ameliorates these deleterious phenotypes. Surprisingly, despite being an ER-resident protein, we show that dnj-27 downregulation alters cytoplasmic protein homeostasis and causes mitochondrial fragmentation. We further demonstrate that DNJ-27 overexpression substantially protects against the mitochondrial fragmentation caused by human Aβ and α-syn peptides in these worm models.INNOVATION: We identify C. elegans dnj-27 as a novel protective gene for the toxicity associated with the expression of human Aβ, α-syn and polyQ proteins, implying a protective role of ERdj5 in Alzheimer, Parkinson and Huntington diseases.CONCLUSION: Our data support a scenario where the levels of DNJ-27/ERdj5 in the ER impact cytoplasmic protein homeostasis and the integrity of the mitochondrial network which might underlie its protective effects in models of proteotoxicity associated to human ND.

KW - Amyloid beta-Peptides

KW - Animals

KW - Animals, Genetically Modified

KW - Autophagy

KW - Caenorhabditis elegans

KW - Caenorhabditis elegans Proteins

KW - Disease Models, Animal

KW - Endoplasmic Reticulum-Associated Degradation

KW - Gene Expression

KW - Gene Expression Regulation

KW - HSP40 Heat-Shock Proteins

KW - Humans

KW - Mitochondria

KW - Molecular Chaperones

KW - Neurodegenerative Diseases

KW - Peptides

KW - Phenotype

KW - Proteasome Endopeptidase Complex

KW - Proteolysis

KW - RNA Interference

KW - alpha-Synuclein

KW - Journal Article

KW - Research Support, N.I.H., Extramural

KW - Research Support, Non-U.S. Gov't

U2 - 10.1089/ars.2012.5051

DO - 10.1089/ars.2012.5051

M3 - Journal article

C2 - 23641861

VL - 20

SP - 217

EP - 235

JO - Antioxidants and Redox Signaling

JF - Antioxidants and Redox Signaling

SN - 1523-0864

IS - 2

ER -