TY - JOUR

T1 - Protein adsorption capability on polyurethane and modified-polyurethane membrane for periodontal guided tissue regeneration applications

AU - Sheikh, Z.

AU - Khan, A.S.

AU - Roohpour, N.

AU - Glogauer, M.

AU - Rehman, I.U.

PY - 2016/11/1

Y1 - 2016/11/1

N2 - Periodontal disease if left untreated can result in creation of defects within the alveolar ridge. Barrier membranes are frequently used with or without bone replacement graft materials for achieving periodontal guided tissue regeneration (GTR). Surface properties of barrier membranes play a vital role in their functionality and clinical success. In this study polyetherurethane (PEU) membranes were synthesized by using 4,4′-methylene-diphenyl diisocyanate (MDI), polytetramethylene oxide (PTMO) and 1,4-butane diol (BDO) as a chain extender via solution polymerization. Hydroxyl terminated polydimethylsiloxane (PDMS) due to having inherent surface orientation towards air was used for surface modification of PEU on one side of the membranes. This resulting membranes had one surface being PEU and the other being PDMS coated PEU. The prepared membranes were treated with solutions of bovine serum albumin (BSA) in de-ionized water at 37 °C at a pH of 7.2. The surface protein adsorptive potential of PEU membranes was observed using Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR), Raman spectroscopy and Confocal Raman spectroscopy. The contact angle measurement, tensile strength and modulus of prepared membranes were also evaluated. PEU membrane (89.86 ± 1.62°) exhibited less hydrophobic behavior than PEU-PDMS (105.87 ± 3.16°). The ultimate tensile strength and elastic modulus of PEU (27 ± 1 MPa and 14 ± 2 MPa) and PEU-PDMS (8 ± 1 MPa and 26 ± 1 MPa) membranes was in required range. The spectral analysis revealed adsorption of BSA proteins on the surface of non PDMS coated PEU surface. The PDMS modified PEU membranes demonstrated a lack of BSA adsorption. The non PDMS coated side of the membrane which adsorbs proteins could potentially be used facing towards the defect attracting growth factors for periodontal tissue regeneration. Whereas, the PDMS coated side could serve as an occlusive barrier for preventing gingival epithelial cells from proliferating and migrating into the defect space by facing the soft tissue flaps. This study demonstrates the potential of a dual natured PEU barrier membrane for use in periodontal tissue engineering applications and further investigations are required. © 2016 Elsevier B.V. All rights reserved.

AB - Periodontal disease if left untreated can result in creation of defects within the alveolar ridge. Barrier membranes are frequently used with or without bone replacement graft materials for achieving periodontal guided tissue regeneration (GTR). Surface properties of barrier membranes play a vital role in their functionality and clinical success. In this study polyetherurethane (PEU) membranes were synthesized by using 4,4′-methylene-diphenyl diisocyanate (MDI), polytetramethylene oxide (PTMO) and 1,4-butane diol (BDO) as a chain extender via solution polymerization. Hydroxyl terminated polydimethylsiloxane (PDMS) due to having inherent surface orientation towards air was used for surface modification of PEU on one side of the membranes. This resulting membranes had one surface being PEU and the other being PDMS coated PEU. The prepared membranes were treated with solutions of bovine serum albumin (BSA) in de-ionized water at 37 °C at a pH of 7.2. The surface protein adsorptive potential of PEU membranes was observed using Attenuated Total Reflectance Fourier Transform Infrared Spectroscopy (ATR-FTIR), Raman spectroscopy and Confocal Raman spectroscopy. The contact angle measurement, tensile strength and modulus of prepared membranes were also evaluated. PEU membrane (89.86 ± 1.62°) exhibited less hydrophobic behavior than PEU-PDMS (105.87 ± 3.16°). The ultimate tensile strength and elastic modulus of PEU (27 ± 1 MPa and 14 ± 2 MPa) and PEU-PDMS (8 ± 1 MPa and 26 ± 1 MPa) membranes was in required range. The spectral analysis revealed adsorption of BSA proteins on the surface of non PDMS coated PEU surface. The PDMS modified PEU membranes demonstrated a lack of BSA adsorption. The non PDMS coated side of the membrane which adsorbs proteins could potentially be used facing towards the defect attracting growth factors for periodontal tissue regeneration. Whereas, the PDMS coated side could serve as an occlusive barrier for preventing gingival epithelial cells from proliferating and migrating into the defect space by facing the soft tissue flaps. This study demonstrates the potential of a dual natured PEU barrier membrane for use in periodontal tissue engineering applications and further investigations are required. © 2016 Elsevier B.V. All rights reserved.

KW - Barrier membranes

KW - FTIR spectroscopy

KW - Mechanical properties

KW - Periodontal tissue engineering

KW - Polyurethanes

KW - Protein adsorption

KW - Raman spectroscopy

KW - Adsorption

KW - Biomechanics

KW - Body fluids

KW - Facings

KW - Fourier transform infrared spectroscopy

KW - Microchannels

KW - Polydimethylsiloxane

KW - Proteins

KW - Silicones

KW - Spectrum analysis

KW - Surface treatment

KW - Tensile strength

KW - Tissue

KW - Tissue regeneration

KW - Attenuated total reflectance fourier transform infrared spectroscopies (ATR FTIR)

KW - Barrier membrane

KW - Confocal Raman spectroscopy

KW - Methylene diphenyl diisocyanate

KW - Periodontal tissue

KW - Protein adsorption capability

KW - Membranes

KW - artificial membrane

KW - bovine serum albumin

KW - polyurethan

KW - adsorption

KW - animal

KW - bovine

KW - chemistry

KW - epithelium cell

KW - gingiva

KW - materials testing

KW - metabolism

KW - periodontal guided tissue regeneration

KW - procedures

KW - synthesis

KW - wettability

KW - Animals

KW - Cattle

KW - Epithelial Cells

KW - Gingiva

KW - Guided Tissue Regeneration, Periodontal

KW - Materials Testing

KW - Membranes, Artificial

KW - Serum Albumin, Bovine

KW - Wettability

U2 - 10.1016/j.msec.2016.05.026

DO - 10.1016/j.msec.2016.05.026

M3 - Journal article

VL - 68

SP - 267

EP - 275

JO - Materials Science and Engineering: A

JF - Materials Science and Engineering: A

SN - 0921-5093

ER -