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    Rights statement: This is a post-peer-review, pre-copyedit version of an article published in Trypanosomatids. The final authenticated version is available online at: http://dx.doi.org/10.1007/978-1-0716-0294-2_10

    Accepted author manuscript, 430 KB, PDF document

    Embargo ends: 30/03/22

    Available under license: CC BY-NC-ND: Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License

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Proteome-wide quantitative phosphoproteomic analysis of Trypanosoma brucei insect and mammalian lifecycle stages

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Abstract

Mass spectrometry based proteomics allows for the identification and quantification of protein and phosphorylation site abundance on a proteome wide scale. Here we describe the metabolic labeling of cultured Trypanosoma brucei cells in either the bloodstream or procyclic life cycle stage using stable isotope labeling of amino acids in cell culture (SILAC), and the production of samples suitable for analysis by liquid chromatography tandem mass spectrometry. The protocols require little specialist equipment, and they typically enable quantification of over 4500 proteins and 9000 phosphorylation sites.

Bibliographic note

This is a post-peer-review, pre-copyedit version of an article published in Trypanosomatids. The final authenticated version is available online at: http://dx.doi.org/10.1007/978-1-0716-0294-2_10