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Proteomic and redox-proteomic evaluation of homogentisic acid and ascorbic acid effects on human articular chondrocytes.

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Proteomic and redox-proteomic evaluation of homogentisic acid and ascorbic acid effects on human articular chondrocytes. / Braconi , Daniela; Laschi, Marcella; Taylor, Adam et al.
In: Journal of Cellular Biochemistry, Vol. 111, No. 4, 01.11.2010, p. 922-932.

Research output: Contribution to Journal/MagazineJournal articlepeer-review

Harvard

Braconi , D, Laschi, M, Taylor, A, Bernardini, G, Spreafico, A, Tinti, L, Gallagher, JA & Santucci, A 2010, 'Proteomic and redox-proteomic evaluation of homogentisic acid and ascorbic acid effects on human articular chondrocytes.', Journal of Cellular Biochemistry, vol. 111, no. 4, pp. 922-932. https://doi.org/10.1002/jcb.22780

APA

Braconi , D., Laschi, M., Taylor, A., Bernardini, G., Spreafico, A., Tinti, L., Gallagher, J. A., & Santucci, A. (2010). Proteomic and redox-proteomic evaluation of homogentisic acid and ascorbic acid effects on human articular chondrocytes. Journal of Cellular Biochemistry, 111(4), 922-932. https://doi.org/10.1002/jcb.22780

Vancouver

Braconi D, Laschi M, Taylor A, Bernardini G, Spreafico A, Tinti L et al. Proteomic and redox-proteomic evaluation of homogentisic acid and ascorbic acid effects on human articular chondrocytes. Journal of Cellular Biochemistry. 2010 Nov 1;111(4):922-932. doi: 10.1002/jcb.22780

Author

Braconi , Daniela ; Laschi, Marcella ; Taylor, Adam et al. / Proteomic and redox-proteomic evaluation of homogentisic acid and ascorbic acid effects on human articular chondrocytes. In: Journal of Cellular Biochemistry. 2010 ; Vol. 111, No. 4. pp. 922-932.

Bibtex

@article{c56ee13c35ee42ad91011348ce33cb16,
title = "Proteomic and redox-proteomic evaluation of homogentisic acid and ascorbic acid effects on human articular chondrocytes.",
abstract = "Alkaptonuria (AKU) is a rare genetic disease associated with the accumulation of homogentisic acid (HGA) and its oxidized/polymerized products in connective tissues up to the deposition of melanin-like pigments (ochronosis). Since little is known on the effects of HGA and its metabolites on articular cells, we carried out a proteomic and redox-proteomic analysis to investigate how HGA and ascorbic acid (ASC) affect the human chondrocytic protein repertoire. We settled up an in vitro model using a human chondrocytic cell line to evaluate the effects of 0.33 mM HGA, alone or combined with ASC. We found that HGA and ASC significantly affect the levels of proteins with specific functions in protein folding, cell organization and, notably, stress response and cell defense. Increased protein carbonyls levels were found either in HGA or ASC treated cells, and evidences produced in this paper support the hypothesis that HGA-induced stress might be mediated by protein oxidation. Our finding can lay the basis towards the settling up of more sophisticated models to study AKU and ochronosis.",
keywords = "alkaptonuria, comparative proteomics, ochronosis, ochronotic arthropathy, post-translational modifications, protein carbonyls, protein oxidation, vitamin C",
author = "Daniela Braconi and Marcella Laschi and Adam Taylor and Giulia Bernardini and Adriano Spreafico and Laura Tinti and Gallagher, {J. A.} and Annalisa Santucci",
year = "2010",
month = nov,
day = "1",
doi = "10.1002/jcb.22780",
language = "English",
volume = "111",
pages = "922--932",
journal = "Journal of Cellular Biochemistry",
issn = "0730-2312",
publisher = "Wiley-Liss Inc.",
number = "4",

}

RIS

TY - JOUR

T1 - Proteomic and redox-proteomic evaluation of homogentisic acid and ascorbic acid effects on human articular chondrocytes.

AU - Braconi , Daniela

AU - Laschi, Marcella

AU - Taylor, Adam

AU - Bernardini, Giulia

AU - Spreafico, Adriano

AU - Tinti, Laura

AU - Gallagher, J. A.

AU - Santucci, Annalisa

PY - 2010/11/1

Y1 - 2010/11/1

N2 - Alkaptonuria (AKU) is a rare genetic disease associated with the accumulation of homogentisic acid (HGA) and its oxidized/polymerized products in connective tissues up to the deposition of melanin-like pigments (ochronosis). Since little is known on the effects of HGA and its metabolites on articular cells, we carried out a proteomic and redox-proteomic analysis to investigate how HGA and ascorbic acid (ASC) affect the human chondrocytic protein repertoire. We settled up an in vitro model using a human chondrocytic cell line to evaluate the effects of 0.33 mM HGA, alone or combined with ASC. We found that HGA and ASC significantly affect the levels of proteins with specific functions in protein folding, cell organization and, notably, stress response and cell defense. Increased protein carbonyls levels were found either in HGA or ASC treated cells, and evidences produced in this paper support the hypothesis that HGA-induced stress might be mediated by protein oxidation. Our finding can lay the basis towards the settling up of more sophisticated models to study AKU and ochronosis.

AB - Alkaptonuria (AKU) is a rare genetic disease associated with the accumulation of homogentisic acid (HGA) and its oxidized/polymerized products in connective tissues up to the deposition of melanin-like pigments (ochronosis). Since little is known on the effects of HGA and its metabolites on articular cells, we carried out a proteomic and redox-proteomic analysis to investigate how HGA and ascorbic acid (ASC) affect the human chondrocytic protein repertoire. We settled up an in vitro model using a human chondrocytic cell line to evaluate the effects of 0.33 mM HGA, alone or combined with ASC. We found that HGA and ASC significantly affect the levels of proteins with specific functions in protein folding, cell organization and, notably, stress response and cell defense. Increased protein carbonyls levels were found either in HGA or ASC treated cells, and evidences produced in this paper support the hypothesis that HGA-induced stress might be mediated by protein oxidation. Our finding can lay the basis towards the settling up of more sophisticated models to study AKU and ochronosis.

KW - alkaptonuria

KW - comparative proteomics

KW - ochronosis

KW - ochronotic arthropathy

KW - post-translational modifications

KW - protein carbonyls

KW - protein oxidation

KW - vitamin C

U2 - 10.1002/jcb.22780

DO - 10.1002/jcb.22780

M3 - Journal article

VL - 111

SP - 922

EP - 932

JO - Journal of Cellular Biochemistry

JF - Journal of Cellular Biochemistry

SN - 0730-2312

IS - 4

ER -