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Purification of β-1, 4-glucanase from ethylene-treated abscission zones of Sambucus nigra

Research output: Contribution to Journal/Magazine › Journal article › peer-review

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Purification of β-1, 4-glucanase from ethylene-treated abscission zones of Sambucus nigra. / Webb, Simon TJ; Taylor, Jane E; Coupe, Simon A et al.
In: Plant, Cell and Environment, Vol. 16, No. 3, 04.1993, p. 329-333.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Webb, STJ, Taylor, JE, Coupe, SA, Tucker, GA, Ferrarese, L & Roberts, JA 1993, 'Purification of β-1, 4-glucanase from ethylene-treated abscission zones of Sambucus nigra', Plant, Cell and Environment, vol. 16, no. 3, pp. 329-333. https://doi.org/10.1111/j.1365-3040.1993.tb00877.x

APA

Webb, S. TJ., Taylor, J. E., Coupe, S. A., Tucker, G. A., Ferrarese, L., & Roberts, J. A. (1993). Purification of β-1, 4-glucanase from ethylene-treated abscission zones of Sambucus nigra. Plant, Cell and Environment, 16(3), 329-333. https://doi.org/10.1111/j.1365-3040.1993.tb00877.x

Vancouver

Webb STJ, Taylor JE, Coupe SA, Tucker GA, Ferrarese L, Roberts JA. Purification of β-1, 4-glucanase from ethylene-treated abscission zones of Sambucus nigra. Plant, Cell and Environment. 1993 Apr;16(3):329-333. doi: 10.1111/j.1365-3040.1993.tb00877.x

Author

Webb, Simon TJ ; Taylor, Jane E ; Coupe, Simon A et al. / Purification of β-1, 4-glucanase from ethylene-treated abscission zones of Sambucus nigra. In: Plant, Cell and Environment. 1993 ; Vol. 16, No. 3. pp. 329-333.

Bibtex

@article{360cc2800719419da04ec45a31cdf947,
title = "Purification of β-1, 4-glucanase from ethylene-treated abscission zones of Sambucus nigra",
abstract = "Leaflet abscission in Sambucus nigra is the result of cell wall breakdown at the site of separation. Associated with wall degradation is an increase in the activity of the enzyme β1,4 glucanase (E.C.3.1.2.4) in the cells that comprise the abscission zone. The enzyme has been extracted from abscission zone tissue and purified using a substrate affinity column. A qualitative enzyme assay procedure has been developed and this has facilitated the purification process. The β1,4 glucanase enzyme has a pH optimum of 7 and a molecular mass of 54kDa. Antibodies have been raised to the purified protein. The role of the enzyme in the abscission process is discussed.",
keywords = "Sambucus nigra, elder, abscission zone , cell separation , β1,4 glucanase , ethylene , antibody",
author = "Webb, {Simon TJ} and Taylor, {Jane E} and Coupe, {Simon A} and Tucker, {Gregory A} and L. Ferrarese and Roberts, {Jeremy A}",
year = "1993",
month = apr,
doi = "10.1111/j.1365-3040.1993.tb00877.x",
language = "English",
volume = "16",
pages = "329--333",
journal = "Plant, Cell and Environment",
issn = "0140-7791",
publisher = "Wiley",
number = "3",

}

RIS

TY - JOUR

T1 - Purification of β-1, 4-glucanase from ethylene-treated abscission zones of Sambucus nigra

AU - Webb, Simon TJ

AU - Taylor, Jane E

AU - Coupe, Simon A

AU - Tucker, Gregory A

AU - Ferrarese, L.

AU - Roberts, Jeremy A

PY - 1993/4

Y1 - 1993/4

N2 - Leaflet abscission in Sambucus nigra is the result of cell wall breakdown at the site of separation. Associated with wall degradation is an increase in the activity of the enzyme β1,4 glucanase (E.C.3.1.2.4) in the cells that comprise the abscission zone. The enzyme has been extracted from abscission zone tissue and purified using a substrate affinity column. A qualitative enzyme assay procedure has been developed and this has facilitated the purification process. The β1,4 glucanase enzyme has a pH optimum of 7 and a molecular mass of 54kDa. Antibodies have been raised to the purified protein. The role of the enzyme in the abscission process is discussed.

AB - Leaflet abscission in Sambucus nigra is the result of cell wall breakdown at the site of separation. Associated with wall degradation is an increase in the activity of the enzyme β1,4 glucanase (E.C.3.1.2.4) in the cells that comprise the abscission zone. The enzyme has been extracted from abscission zone tissue and purified using a substrate affinity column. A qualitative enzyme assay procedure has been developed and this has facilitated the purification process. The β1,4 glucanase enzyme has a pH optimum of 7 and a molecular mass of 54kDa. Antibodies have been raised to the purified protein. The role of the enzyme in the abscission process is discussed.

KW - Sambucus nigra

KW - elder

KW - abscission zone

KW - cell separation

KW - β1,4 glucanase

KW - ethylene

KW - antibody

U2 - 10.1111/j.1365-3040.1993.tb00877.x

DO - 10.1111/j.1365-3040.1993.tb00877.x

M3 - Journal article

VL - 16

SP - 329

EP - 333

JO - Plant, Cell and Environment

JF - Plant, Cell and Environment

SN - 0140-7791

IS - 3

ER -