Rac1 drives melanoblast organization during mouse development by orchestrating pseudopod- driven motility and cell-cycle progression

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https://doi.org/10.1016/j.devcel.2011.07.008
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Rac1 drives melanoblast organization during mouse development by orchestrating pseudopod- driven motility and cell-cycle progression. / Li, Ang; Ma, Yafeng; Yu, Xinzi et al.
In: Developmental Cell, Vol. 21, No. 4, 18.10.2011, p. 722-734.

Research output: Contribution to Journal/Magazine › Journal article › peer-review

Harvard

Li, A, Ma, Y, Yu, X, Mort, RL, Lindsay, CR, Stevenson, D, Strathdee, D, Insall, RH, Chernoff, J, Snapper, SB, Jackson, IJ, Larue, L, Sansom, OJ & Machesky, LM 2011, 'Rac1 drives melanoblast organization during mouse development by orchestrating pseudopod- driven motility and cell-cycle progression', Developmental Cell, vol. 21, no. 4, pp. 722-734. https://doi.org/10.1016/j.devcel.2011.07.008

APA

Li, A., Ma, Y., Yu, X., Mort, R. L., Lindsay, C. R., Stevenson, D., Strathdee, D., Insall, R. H., Chernoff, J., Snapper, S. B., Jackson, I. J., Larue, L., Sansom, O. J., & Machesky, L. M. (2011). Rac1 drives melanoblast organization during mouse development by orchestrating pseudopod- driven motility and cell-cycle progression. Developmental Cell, 21(4), 722-734. https://doi.org/10.1016/j.devcel.2011.07.008

Vancouver

Li A, Ma Y, Yu X, Mort RL, Lindsay CR, Stevenson D et al. Rac1 drives melanoblast organization during mouse development by orchestrating pseudopod- driven motility and cell-cycle progression. Developmental Cell. 2011 Oct 18;21(4):722-734. Epub 2011 Sept 15. doi: 10.1016/j.devcel.2011.07.008

Author

Li, Ang ; Ma, Yafeng ; Yu, Xinzi et al. / Rac1 drives melanoblast organization during mouse development by orchestrating pseudopod- driven motility and cell-cycle progression. In: Developmental Cell. 2011 ; Vol. 21, No. 4. pp. 722-734.

Bibtex

@article{249ab848630a426cac5a89fae08d5e7e,

title = "Rac1 drives melanoblast organization during mouse development by orchestrating pseudopod- driven motility and cell-cycle progression",

abstract = "During embryogenesis, melanoblasts proliferate and migrate ventrally through the developing dermis and epidermis as single cells. Targeted deletion of Rac1 in melanoblasts during embryogenesis causes defects in migration, cell-cycle progression, and cytokinesis. Rac1 null cells migrate markedly less efficiently, but surprisingly, global steering, crossing the dermal/epidermal junction, and homing to hair follicles occur normally. Melanoblasts navigate in the epidermis using two classes of protrusion: short stubs and long pseudopods. Short stubs are distinct from blebs and are driven by actin assembly but are independent of Rac1, Arp2/3 complex, myosin, or microtubules. Rac1 positively regulates the frequency of initiation of long pseudopods, which promote migration speed and directional plasticity. Scar/WAVE and Arp2/3 complex drive actin assembly for long pseudopod extension, which also depends on microtubule dynamics. Myosin contractility balances the extension of long pseudopods by effecting retraction and allowing force generation for movement through the complex 3D epidermal environment.",

keywords = "Actins, Animals, Cell Cycle, Cell Movement, Cell Proliferation, Cells, Cultured, Embryo, Mammalian, Epidermis, Female, Flow Cytometry, Gene Expression Regulation, Developmental, Hair Follicle, Integrases, Male, Melanocytes, Mice, Mice, Inbred C57BL, Mice, Transgenic, Microtubules, Myosins, Neuropeptides, Pseudopodia, Skin, rac GTP-Binding Proteins, rac1 GTP-Binding Protein, Journal Article, Research Support, N.I.H., Extramural, Research Support, Non-U.S. Gov't",

author = "Ang Li and Yafeng Ma and Xinzi Yu and Mort, {Richard L.} and Lindsay, {Colin R.} and David Stevenson and Douglas Strathdee and Insall, {Robert H.} and Jonathan Chernoff and Snapper, {Scott B.} and Jackson, {Ian J.} and Lionel Larue and Sansom, {Owen J.} and Machesky, {Laura M.}",

year = "2011",

month = oct,

day = "18",

doi = "10.1016/j.devcel.2011.07.008",

language = "English",

volume = "21",

pages = "722--734",

journal = "Developmental Cell",

issn = "1534-5807",

publisher = "Cell Press",

number = "4",

}

RIS

TY - JOUR

T1 - Rac1 drives melanoblast organization during mouse development by orchestrating pseudopod- driven motility and cell-cycle progression

AU - Li, Ang

AU - Ma, Yafeng

AU - Yu, Xinzi

AU - Mort, Richard L.

AU - Lindsay, Colin R.

AU - Stevenson, David

AU - Strathdee, Douglas

AU - Insall, Robert H.

AU - Chernoff, Jonathan

AU - Snapper, Scott B.

AU - Jackson, Ian J.

AU - Larue, Lionel

AU - Sansom, Owen J.

AU - Machesky, Laura M.

PY - 2011/10/18

Y1 - 2011/10/18

N2 - During embryogenesis, melanoblasts proliferate and migrate ventrally through the developing dermis and epidermis as single cells. Targeted deletion of Rac1 in melanoblasts during embryogenesis causes defects in migration, cell-cycle progression, and cytokinesis. Rac1 null cells migrate markedly less efficiently, but surprisingly, global steering, crossing the dermal/epidermal junction, and homing to hair follicles occur normally. Melanoblasts navigate in the epidermis using two classes of protrusion: short stubs and long pseudopods. Short stubs are distinct from blebs and are driven by actin assembly but are independent of Rac1, Arp2/3 complex, myosin, or microtubules. Rac1 positively regulates the frequency of initiation of long pseudopods, which promote migration speed and directional plasticity. Scar/WAVE and Arp2/3 complex drive actin assembly for long pseudopod extension, which also depends on microtubule dynamics. Myosin contractility balances the extension of long pseudopods by effecting retraction and allowing force generation for movement through the complex 3D epidermal environment.

AB - During embryogenesis, melanoblasts proliferate and migrate ventrally through the developing dermis and epidermis as single cells. Targeted deletion of Rac1 in melanoblasts during embryogenesis causes defects in migration, cell-cycle progression, and cytokinesis. Rac1 null cells migrate markedly less efficiently, but surprisingly, global steering, crossing the dermal/epidermal junction, and homing to hair follicles occur normally. Melanoblasts navigate in the epidermis using two classes of protrusion: short stubs and long pseudopods. Short stubs are distinct from blebs and are driven by actin assembly but are independent of Rac1, Arp2/3 complex, myosin, or microtubules. Rac1 positively regulates the frequency of initiation of long pseudopods, which promote migration speed and directional plasticity. Scar/WAVE and Arp2/3 complex drive actin assembly for long pseudopod extension, which also depends on microtubule dynamics. Myosin contractility balances the extension of long pseudopods by effecting retraction and allowing force generation for movement through the complex 3D epidermal environment.

KW - Actins

KW - Animals

KW - Cell Cycle

KW - Cell Movement

KW - Cell Proliferation

KW - Cells, Cultured

KW - Embryo, Mammalian

KW - Epidermis

KW - Female

KW - Flow Cytometry

KW - Gene Expression Regulation, Developmental

KW - Hair Follicle

KW - Integrases

KW - Male

KW - Melanocytes

KW - Mice

KW - Mice, Inbred C57BL

KW - Mice, Transgenic

KW - Microtubules

KW - Myosins

KW - Neuropeptides

KW - Pseudopodia

KW - Skin

KW - rac GTP-Binding Proteins

KW - rac1 GTP-Binding Protein

KW - Journal Article

KW - Research Support, N.I.H., Extramural

KW - Research Support, Non-U.S. Gov't

U2 - 10.1016/j.devcel.2011.07.008

DO - 10.1016/j.devcel.2011.07.008

M3 - Journal article

C2 - 21924960

VL - 21

SP - 722

EP - 734

JO - Developmental Cell

JF - Developmental Cell

SN - 1534-5807

IS - 4

ER -

Research

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