Home > Research > Publications & Outputs > RAD51 Is a Selective DNA Repair Target to Radio...


Text available via DOI:

View graph of relations

RAD51 Is a Selective DNA Repair Target to Radiosensitize Glioma Stem Cells

Research output: Contribution to journalJournal article

  • Harry O. King
  • Tim Brend
  • Helen L. Payne
  • Alexander Wright
  • Thomas A. Ward
  • Karan Patel
  • Teklu Egnuni
  • Lucy F. Stead
  • Anjana Patel
  • Heiko Wurdak
  • Susan C. Short
<mark>Journal publication date</mark>10/01/2017
<mark>Journal</mark>Stem Cell Reports
Issue number1
Number of pages15
Pages (from-to)125-139
Publication statusPublished
Original languageEnglish


Patients with glioblastoma die from local relapse despite surgery and high-dose radiotherapy. Resistance to radiotherapy is thought to be due to efficient DNA double-strand break (DSB) repair in stem-like cells able to survive DNA damage and repopulate the tumor. We used clinical samples and patient-derived glioblastoma stem cells (GSCs) to confirm that the DSB repair protein RAD51 is highly expressed in GSCs, which are reliant on RAD51-dependent DSB repair after radiation. RAD51 expression and RAD51 foci numbers fall when these cells move toward astrocytic differentiation. In GSCs, the small-molecule RAD51 inhibitors RI-1 and B02 prevent RAD51 focus formation, reduce DNA DSB repair, and cause significant radiosensitization. We further demonstrate that treatment with these agents combined with radiation promotes loss of stem cells defined by SOX2 expression. This indicates that RAD51-dependent repair represents an effective and specific target in GSCs.