Research output: Contribution to Journal/Magazine › Journal article › peer-review
Research output: Contribution to Journal/Magazine › Journal article › peer-review
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TY - JOUR
T1 - Regulation of Arabidopsis 14-3-3 gene expression by GABA.
AU - Lancien, Muriel
AU - Roberts, Michael R
N1 - The definitive version is available at www.blackwell-synergy.com (c) Blackwell 2006.
PY - 2006/7
Y1 - 2006/7
N2 - The function in plants of the non-protein amino acid, gamma-aminobutyric acid (GABA) is poorly understood. In this study, we show that GABA down-regulates the expression of a large sub-set of 14-3-3 gene family members in Arabidopsis thaliana seedlings in a calcium, ethylene and abscisic acid-dependent manner. Gene expression is not affected when seedlings are supplied with glutamate, a precursor of GABA. The repression of 14-3-3 gene expression by GABA is dependent on functional ethylene and abscisic acid signalling pathways, since the response is lost in the etr1-1, abi1-1 and abi2-1 mutants. Calcium measurements show that in contrast to glutamate, GABA does not elicit a cytoplasmic calcium elevation, suggesting that the GABA response is unlikely to be mediated by glutamate receptors, as has been suggested previously. We suggest that in addition to its role as a stress-related metabolite, GABA may regulate gene expression in Arabidopsis, including members of the 14-3-3 gene family.
AB - The function in plants of the non-protein amino acid, gamma-aminobutyric acid (GABA) is poorly understood. In this study, we show that GABA down-regulates the expression of a large sub-set of 14-3-3 gene family members in Arabidopsis thaliana seedlings in a calcium, ethylene and abscisic acid-dependent manner. Gene expression is not affected when seedlings are supplied with glutamate, a precursor of GABA. The repression of 14-3-3 gene expression by GABA is dependent on functional ethylene and abscisic acid signalling pathways, since the response is lost in the etr1-1, abi1-1 and abi2-1 mutants. Calcium measurements show that in contrast to glutamate, GABA does not elicit a cytoplasmic calcium elevation, suggesting that the GABA response is unlikely to be mediated by glutamate receptors, as has been suggested previously. We suggest that in addition to its role as a stress-related metabolite, GABA may regulate gene expression in Arabidopsis, including members of the 14-3-3 gene family.
KW - 14-3-3 protein GABA Arabidopsis thaliana plant stress
U2 - 10.1111/j.1365-3040.2006.01526.x
DO - 10.1111/j.1365-3040.2006.01526.x
M3 - Journal article
VL - 29
SP - 1430
EP - 1436
JO - Plant, Cell and Environment
JF - Plant, Cell and Environment
SN - 0140-7791
IS - 7
ER -